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بررسی اثر ضداسپاسم عصاره و اسانس آویشن دنائی روی انقباضات رحم رات | ||
| پژوهش های پیشرفته در گیاهان دارویی | ||
| دوره 2، شماره 3 - شماره پیاپی 4، آذر 1404، صفحه 33-43 اصل مقاله (1.01 M) | ||
| نوع مقاله: مقاله علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30479/armp.2025.21772.1043 | ||
| نویسندگان | ||
| حسن صدرائی* 1؛ مائده قاسمی2؛ غزل دهقان دهکردی3؛ افسانه یگدانه1 | ||
| 1دانشیار، گروه فارماکولوژی و سمشناسی، دانشکده داروسازی و علوم دارویی، دانشگاه علوم پزشکی اصفهان، اصفهان | ||
| 2استادیار، گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان | ||
| 3دکتری، گروه فارماکولوژی و سمشناسی، دانشکده داروسازی و علوم دارویی، دانشگاه علوم پزشکی اصفهان، اصفهان | ||
| تاریخ دریافت: 25 اسفند 1403، تاریخ بازنگری: 21 تیر 1404، تاریخ پذیرش: 05 مهر 1404 | ||
| چکیده | ||
| آویشندنائی (T. daenensis) یکی از گونههای بومی آویشن ایران است که کاربرد داروئی دارد. هدف از انجام این تحقیق، ارزیابی اثر عصاره و اسانس آویشن دنائی روی انقباضات عضلات صاف رحم بود. به این منظور، بافت رحم جداشده از رات در حمام بافت قرار داده شد و انقباضات توسط اسپاسموژنهای KCl، استیلکولین و اکسیتوسین ایجاد گردید. اثرات عصاره هیدروالکلی، پارتیشن هگزانی، اسانس و نیفدیپین بر روی انقباضات رحمی ناشی از محرکهای فوق، مورد بررسی قرارگرفت. نیفدیپین انقباضات رحمی ناشی از KCl، استیلکولین و اکسیتوسین را مهار کرد. عصاره هیدروالکلی، پارتیشن هگزانی و اسانس آویشن دنائی از غلظت µg/ml 20 تا حداکثر mg/ml 28/1 اثر مهاری وابسته به غلظت، روی انقباضات ناشی از KCl، استیلکولین و اکسیتوسین بر بافت رحم جداشده رات، نشان دادند. آویشن دنائی اثر ضداسپاسم روی انقباضات رحم دارد. مقایسه عصاره هیدروالکلی با پارتیشن هگزانی گیاه، نشان داد که پارتیشن هگزانی اثر مهاری قویتری نسبت به عصاره هیدروالکلی دارد. همچنین، اثر آنتی اسپاسم اسانس، بسیار قویتر از پارتیشنهای هیدروالکلی و هگزانی بود. با توجه به نتایج، به نظر میرسد که ترکیبات لیپوفیلیک موجود در اسانس و پارتیشن هگزانی عامل اصلی اثرات ضداسپاسم گیاه آویشن دنائی باشند. | ||
| کلیدواژهها | ||
| آنتی اسپاسم؛ آویشن دنائی؛ رحم؛ عضلات صاف | ||
| عنوان مقاله [English] | ||
| valuation of antispasmodic effect of exracts and essential oil of Thymus daenensis L. on rat uterus contractions | ||
| نویسندگان [English] | ||
| Hassan Sadraei1؛ Maedeh Ghasemi2؛ Ghazal Dehgan Dehkordi3؛ , Afsaneh Yegdaneh1 | ||
| 11Associate Professor, Dept. of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 2Assistant Professor, Dept. of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 3Ph.D, Dept. of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده [English] | ||
| Thymus daenensis L. is a local species of Lamiaceae family found in Iran. T. daenensis traditionally has been used by local people. The aim of this research was to assess spasmolytic action of T. daenensis hydroalcoholic extract, hexane partition and the essential oil on uterus contraction. For pharmacological studies, isolated rat uterus suspended in an organ bath filled by Tyrode’s solution, was contracted by KCl, acetylcholine or oxytocin. Inhibitory action of T. daenensis extracts and essential oil was examined on above spasmogens in rat uterus and recorded on oscillograph apparatus. Nifedipine was used as standard drugs. Nifedipine in a concentration manner inhibited rat uterus contractions induced by KCl, acetylcholine and oxytocin. The hydroalcoholic extract (20µg/ml-1.28mg/ml), hexane partition and essential oil of T. daenensis also attenuated uterine contractions induced by above spasmogens in the organ bath. Comparing the inhibitory effect of hydroalcoholic extract with hexane partition indicate that the hexane partition contains more potent components. The essential oil of T. daenensis was even more potent than the hexane partition. Therefore, it looks like that the lipophilic constituents in the hexane partition and the essential oil are mainly responsible for inhibitory action of T. daenensis. | ||
| کلیدواژهها [English] | ||
| Antispasm, Smooth muscle, Thymus daenensis, Uterus | ||
| مراجع | ||
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