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Towards conservation and breeding of Ajowan (Trachyspermum ammi) by assessing ISSR, morphological traits and germination variability | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 2، دوره 5، شماره 1 - شماره پیاپی 9، تیر 2016، صفحه 8-21 اصل مقاله (1.1 M) | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2016.1039 | ||
نویسندگان | ||
Sina Sadati1؛ Seyed Ahmad Sadat-Noori2؛ Hossein Ramshini* 3؛ Elias Soltani3؛ Behroz Foghi4 | ||
1Graduate Student of Plant Breeding, Department of Agronomy and Plant Breeding Sciences, College of Abouraihan, University of Tehran, Iran. | ||
2Professor, Department of Agronomy and Plant Breeding Sciences, College of Abouraihan, University of Tehran, Iran. | ||
3Asistant Professor, Department of Agronomy and Plant Breeding Sciences, College of Abouraihan, University of Tehran, P. O. Box: 33916-53755. | ||
4M.Sc., Department of Agronomy and Plant Breeding Sciences, College of Abouraihan, University of Tehran, Iran. | ||
تاریخ دریافت: 06 اردیبهشت 1396، تاریخ پذیرش: 06 اردیبهشت 1396 | ||
چکیده | ||
Ajowan is one of the most important members of medicinal plants of the Apiaceae, consumed by humans. The aim of this study was to explore genetic variability of 20 ajowan populations by ISSR fingerprinting and evaluation of traits at germination. Out of the 30 ISSR primers, ten generated reproducible and polymorphic bands. A total of 93 bands were detected out of which 65 were polymorphic. Highest values for resolving power (Rp) were detected for UBC818 and ISSR5 primers (7.2 and 4.6, respectively) which their sequences had (AC) n or (CA) n motifs. All genotypes were separated in dendrogram drawn by the ISSR data. For all traits measured at the germination stage, there were highly significant differences among genotypes (P≤0.01). A low germination percentage (42.1%) was observed in average. Qom was the best population considering germination properties such as germination percentage (95%) and shoot length (3.8 cm). The highest number of branches and number of umbels were observed for Sabzevar (with 83 and 91, respectively). High broad sense heritability values were obtained for germination and morphological traits (ranging from 0.68 to 0.99). In conclusion, two populations of Sabzevar and Qom populations were the best for several traits and are suggested for further analysis. | ||
کلیدواژهها | ||
Cluster analysis؛ Domestication؛ Genetic diversity؛ Heritability؛ Molecular markers | ||
عنوان مقاله [English] | ||
ارزیابی تنوع ISSR، مورفولوژی و جوانه زنی زنیان (Trachyspermum ammi) به منظور کمک به اصلاح و حفاظت گیاه | ||
نویسندگان [English] | ||
سینا ساداتی1؛ سید احمد سادات نوری2؛ حسین رامشینی3؛ الیاس سلطانی3؛ بهروز فوقی4 | ||
1دانش آموخته کارشناسی ارشد رشته اصلاح نباتات، گروه علوم زراعی و اصلاح نباتات، پردیس ابوریحان، دانشگاه تهران، ایران. | ||
2استاد گروه علوم زراعی و اصلاح نباتات، پردیس ابوریحان، دانشگاه تهران، ایران. | ||
3استادیار گروه علوم زراعی و اصلاح نباتات، پردیس ابوریحان، دانشگاه تهران، ایران، کدپستی: 53755-33916. | ||
4مربی گروه علوم زراعی و اصلاح نباتات، پردیس ابوریحان، دانشگاه تهران، ایران. | ||
چکیده [English] | ||
زنیان یکی از مهمترین گیاهان دارویی خانواده چتریان است که توسط انسان به مصرف میرسد. هدف این مطالعه بررسی تنوع ژنتیکی 20 جمعیت زنیان با کمک انگشت نگاری ISSR و ارزیابی صفات در مرحله جوانهزنی و گیاه بالغ بود. از 30 آغازگر ISSR، 10 عدد تولید نوارهای چند شکل تکرار پذیر کردند. در مجموع 93 نوار شناسایی شد که 65 نوار چند شکل بودند. بیشترین مقدار قدرت تفکیک برای آغازگرهای UBC818 و ISSR5 ( با Rp به ترتیب برابر 2/7 و 6/4) به دست آمد که توالی آنها دارای تکرارهای AC)n) و CA)n) بودند. همه ژنوتیپها در نمودار درختی رسم شده با دادههای ISSR از یکدیگر تفکیک شدند. برای همه صفات اندازه گیری شده در مرحله جوانهزنی تفاوتهای بسیار معنی دار (p≤0.01) بین ژنوتیپها وجود داشت. میانگین درصد جوانهزنی (1/42%) در بین ژنوتیپها پایین بود. جمعیت قم بهترین جمعیت از نظر ویژگیهای جوانهزنی مانند درصد جوانهزنی (95%) و طول ساقه چه (8/3 سانتی متر) بود. بیشترین تعداد شاخه و تعداد چتر در جمعیت سبزوار (به ترتیب 83 و 91) به دست آمد. توارث پذیری عمومی بالا برای صفات جوانهزنی و مورفولوژیک ملاحظه شد (بین 68/0 تا 99/0). در مجموع دو جمعیت سبزوار و قم از نظر چندین صفت بهترین جمعیتها بودند و برای مطالعات بیشتر پیشنهاد میشوند. | ||
کلیدواژهها [English] | ||
تجزیه کلاستر, اهلی سازی, تنوع ژنتیکی, توارث پذیری, نشانگرهای مولکولی | ||
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