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Morphological and molecular responses of sunflower (Helianthus annuus L.) lines to drought stress | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 5، دوره 5، شماره 1 - شماره پیاپی 9، تیر 2016، صفحه 40-56 اصل مقاله (1022.97 K) | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2016.1042 | ||
نویسندگان | ||
Mozhgan Sarvari1؛ Reza Darvishzadeh* 2؛ Roghayeh Najafzadeh3 | ||
1Department of Biology, Urmia University, Urmia, Iran. | ||
2Department of Plant Breeding and Biotechnology, Urmia University, P. O. Box: 165, Urmia, Iran. | ||
3Department of Medicinal Plants, Shahid Bakeri Higher Education Center, Urmia University, Miyandoab, Iran. | ||
تاریخ دریافت: 06 اردیبهشت 1396، تاریخ پذیرش: 06 اردیبهشت 1396 | ||
چکیده | ||
Drought stress is a serious adverse factor limiting growth and productivity of sunflower. Evaluation of defense systems is important for producing resistant cultivars. In this study, some agro-morphological and molecular characteristics of six sunflower lines were evaluated under normal and irrigation at 40 and 60% of field capacity in randomized complete block design (RCBD). The results showed that drought stress affected the measured traits compared to the control. However, the lines showed different responses to the stress. According to the results, C100, C104 and LR55 lines had better growth, yield and drought tolerance indices in water stress conditions. In addition, drought stress increased total protein content of the lines especially resistant ones (C100, C104 and LR55). The protein SDS-PAGE electrophoresis pattern also showed that drought stress caused the accumulation of low molecular weight proteins specially the bands 69.3 and 70.5 kDa were produced in the resistant lines C100, C104 and LR55 under drought stress conditions. Moreover, more protein bands were observed in the resistant lines, C104 and LR55, under normal compared to the stress conditions. It seems that the protein bands 69.3 and 70.5 kDa are resistance-related proteins that were expressed in the tolerant lines. The findings of this study can be useful in sunflower breeding programs for producing resistant cultivars to drought stress. | ||
کلیدواژهها | ||
Abiotic stress؛ Drought tolerance indices؛ Oil crops؛ SDS-PAGE؛ Seed yield | ||
عنوان مقاله [English] | ||
پاسخ های مورفولوژیکی و مولکولی لاین های آفتابگردان (.Helianthus annuus L) به تنش خشکی | ||
نویسندگان [English] | ||
مژگان سروری1؛ رضا درویش زاده2؛ رقیه نجف زاده3 | ||
1گروه زیست شناسی، دانشکده علوم پایه، دانشگاه ارومیه، ارومیه، ایران. | ||
2گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه ، ایران، کدپستی: 165. | ||
3گروه گیاهان دارویی، مرکز آموزش عالی شهید باکری، دانشگاه ارومیه، میاندوآب، ایران. | ||
چکیده [English] | ||
تنش خشکی یک عامل مخرب جدّی میباشد که باعث کاهش رشد و عملکرد آفتابگردان میشود. از این رو بررسی سیستم دفاعی این گیاه جهت تولید ارقام مقاوم حائز اهمیت میباشد. در پژوهش حاضر برخی خصوصیات مورفولوژیکی و مولکولی 6 لاین آفتابگردان تحت شرایط نرمال و آبیاری در 40 و 60 درصد ظرفیت زراعی در قالب طرح بلوکهای کامل تصادفی ارزیابی شد. نتایج نشان داد که تنش خشکی بر خصوصیات مورفولوژیکی و مولکولی گیاهان در مقایسه با تیمار شاهد اثر گذاشت. همچنین ژنوتیپها نیز پاسخهای متفاوتی در برابر تنش از خود نشان دادند. بر اساس این نتایج، ژنوتیپهای C100، C104 و LR55 خصوصیات رشدی، عملکردی و شاخصهای تحمل به خشکی بهتری در شرایط تنش خشکی داشتند. علاوه بر این، تنش خشکی میزان پروتئین را بخصوص در ژنوتیپهای مقاوم (C100، C104 و LR55) افزایش داد. الگوی الکتروفورزی SDS-PAGE پروتئین نشان داد که تنش خشکی باعث تجمع پروتئینهایی با وزن مولکولی کم شد. بخصوص باعث تولید باندهای پروتئینی 3/69 و 5/70 کیلودالتون در ژنوتیپهای مقاوم (C100، C104 و LR55) در شرایط تنش خشکی شد. تعداد باندهای پروتئینی در ژنوتیپهای مقاوم C104 و LR55 نیز تحت شرایط نرمال در مقایسه با تنش خشکی بیشتر بود. به نظر میرسد که باندهای پروتئینی 3/69 و 5/70 کیلودالتون پروتئینهای مقاومت هستند که در ژنوتیپهای مقاوم بیان شده اند. نتایج حاصل از این پژوهش میتواند در برنامههای به نژادی آفتابگردان جهت تولید ارقام مقاوم به خشکی مفید باشد. | ||
کلیدواژهها [English] | ||
تنش غیر زیستی, شاخص های تحمل به خشکی, گیاهان روغنی, SDS-PAGE, عملکرد دانه | ||
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