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Study of new NBS-LRR genes analogues in cucurbits native types in Iran | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 2، دوره 6، شماره 1 - شماره پیاپی 11، تیر 2017، صفحه 8-15 اصل مقاله (485.94 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2017.1369 | ||
| نویسندگان | ||
| Fatemeh Gharaei1، 2؛ Maryam Ghayeb Zamharir* 1 | ||
| 1Department of Plant Diseases, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), P. O. Box: 19395-1454, Tehran, Iran. | ||
| 2Science and Research Branch of Islamic Azad University, Tehran, Iran. | ||
| تاریخ دریافت: 21 خرداد 1397، تاریخ پذیرش: 21 خرداد 1397 | ||
| چکیده | ||
| Nucleotide binding site leucine-rich repeats (NBS-LRR) accounting for the main disease resistance proteins play an important role in plant defense against pathogen attack. The current study aimed to identify new NBS-LRR gene members in native types of cucurbit species in Iran. Accordingly, DNAs of melon, cucumber and cantaloupe native types to Iran were identified using three primer pairs. PCR products of the expected size were generated and the obtained DNA was cloned using the pGEM-T. BLASTN algorithms were used to compare the insert sequences with sequences available at the Entrez nucleotide and protein. Phylogenetic analyses were conducted using the MEGA software. The results analysis suggests that cucurbit species native types’ genome contain new NBS_LRR resistance gene analogues against cucurbit pathogens. These resistance gene analogues are composed of genes related to both coiled-coil (CC) and toll-interleukin-receptor homology (TIR) domain containing NBS-LRR R-genes. Phylogenetic analysis of these genes shows that they include NBS-LRRs derived from a recent common ancestor. This study provides an insight into the evolution of NBS-LRR genes in the Cucurbit native type species genomes that are resistance analogues against cucurbit bacterial blight pathogen and tomato mosaic virus. | ||
| کلیدواژه‌ها | ||
| Cucurbits species؛ Iran؛ Native types؛ NBS-LRR | ||
| عنوان مقاله [English] | ||
| مطالعه آنالوگهای جدید مقاومت متعلق به NBS-LRR در کدوئیان بومی ایران | ||
| نویسندگان [English] | ||
| فاطمه قرائی1، 2؛ مریم غایب زمهریر1 | ||
| 1آزمایشگاه پروکاریوت شناسی، بخش تحقیقات بیماری‌های گیاهی، موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران، کدپستی: 1454-19395. | دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران. | ||
| 2آزمایشگاه پروکاریوت شناسی، بخش تحقیقات بیماری‌های گیاهی، موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران، کدپستی: 1454-19395. | دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران. | ||
| چکیده [English] | ||
| NBS_LRR از اصلی‌ترین پروتئین‌های مقاوم به بیماری در گیاهان هستند که نقشی مهم در دفاع گیاهان بر ضد پاتوژن‌ها برعهده دارند. هدف از این پژوهش، تشخیص اعضای جدید ژن‌های خانوادة NBS-LRR در گونه‌های کدوئیان بومی ایران است. برای این منظور، DNAs از برگ ارقام بومی خربزه، طالبی و خیار ایران استخراج و با استفاده از سه ترکیب پرایمری تکثیر شدند و فرآورده‌های حاصل از واکنش در وکتور pGEM-T همانندسازی شدند. برای مقایسة توالی‌های حاصل از این واکنش با توالی‌ها و پروتئین‌های موجود در بانک ژن، از نرم افزار BLASTN استفاده شد و آنالیزهای فیلوژنتیکی با استفاده از نرم افزار MEGA انجام شد. نتایج آنالیزها نشان می‌دهد که ژنوم گونه‌های کدوئیان بومی، حاوی آنالوگهای جدید مقاوم به بیماری از خانواده NBS_LRR هستند. این آنالوگهای ژن مقاوم به بیماری در هر دو زیرخانواده coiled-coil (CC) وtoll-interleukin-receptor homology (TIR) شناسایی شدند. بررسی فیلوژنتیک این ژن‌ها نشان داد که آنالوگهای مقاومت از ریشه یا تباری مشترک نشأت گرفته‌اند. این جستار، اطلاعات جدید برای تکمیل مطالعة ژن‌های مقاوم به بیماری را در خانوادة BS-LRR فراهم نموده است که می‌تواند در مقابل بیماری بلایت باکتریایی کدوئیان و ویروس موزائیک گوجه فرنگی مقاومت ایجاد کند. | ||
| کلیدواژه‌ها [English] | ||
| ایران, بومی, گونه های کدوئیان, NBS-LRR | ||
| مراجع | ||
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