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Effects of zeatin riboside, mannitol and heat stress on eggplantn (Solanum melongena L.) anther culture | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 3، دوره 6، شماره 1 - شماره پیاپی 11، تیر 2017، صفحه 16-26 اصل مقاله (653.33 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2017.1370 | ||
نویسندگان | ||
Masoud Emrani Dehkehan1؛ Ahmad Moieni* 1؛ Zahra Movahedi2 | ||
1Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, P. O. Box: 14115336, Tehran, Iran. | ||
2Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer, Iran. | ||
تاریخ دریافت: 21 خرداد 1397، تاریخ پذیرش: 21 خرداد 1397 | ||
چکیده | ||
In this research, the effects of zeatin riboside (0.6, 0.8, 1, 1.2 and 1.4 mg l-1), duration of heat stress of 35 °C (6, 8, 10 and 12 days) and mannitol (0, 10, 20, 30 and 40 mg l-1) on anther culture of four eggplant cultivars were investigated in independent experiments. These experiments were performed according to a factorial arrangement in a randomized complete blocks design layout. The results showed significant interaction effects between the cultivars and treatments. The highest percentage of embryo-derived plantlets (25%) was obtained in cultivar Chantal using 1 mg l-1 zeatin riboside and 8 days of the heat stress. The results also indicated that the use of 10 mg l-1 mannitol had the highest embryo-derived plantlets (66.6%) in cultivar Chantal. In cultivars Hadrian and Faselis, the ploidy levels of all regenerated plants from anther culture (10 and 21 plants, respectively) were determined through flow cytometry and it was found that 60% and 57.1% of the tested plants were haploid, respectively. In cultivars Chantale and Valentina, 30 regenerated plants were selected for determining their ploidy levels and the results showed that 40.2% and 51.5% of the plants were haploid, respectively. | ||
کلیدواژهها | ||
Androgenesis؛ Haploidy؛ Osmotic stress | ||
عنوان مقاله [English] | ||
اثر زئاتین ریبوزاید، مانیتول و تنش حرارتی در کشت بساک بادمجان (.Solanum melongena L) | ||
نویسندگان [English] | ||
مسعود عمرانی دهکهان1؛ احمد معینی1؛ زهرا موحدی2 | ||
1گروه اصلاح نباتات، دانشکدۀ کشاورزی، دانشگاه تربیت مدرس، تهران، ایران، کدپستی: 14115336. | ||
2گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی، دانشگاه ملایر، ملایر، ایران. | ||
چکیده [English] | ||
در این تحقیق، آثار غلظت زئاتین ریبوزاید (0.6, 0.8, 1, 1.2 and 1.4 mg l-1)، مدت زمان تنش دمای35 °C (6، 8، 10 و 12 روز) و غلظت مانیتول (0, 10, 20, 30 and 40 mg l-1) روی کشت بساک چهار رقم بادمجان بررسی شد. بر اساس نتایج تجزیة واریانس، اثر متقابل بین ارقام و تیمارها معنیدار بود و بیشترین درصد گیاهچه های باززایی شده حاصل از جنین (25%) با استفاده از1 mg l-1 زئاتین ریبوزاید و 8 روز تنش حرارتی در رقم شانتال به دست آمد. همچنین نتایج نشان داد که استفاده از10 mg l-1 مانیتول، منجر به تولید بیشترین درصد گیاهچههای باززایی شده حاصل از جنین (6/66 %) در رقم شانتال شده است. در ارقام هاردیان و فاسلیس، سطوح پلوییدی همه گیاهان حاصل از کشت بساک (به ترتیب 10 و 21 گیاه) از طریق فلوسایتومتری تعیین شدند و بر اساس نتایج، به ترتیب 60% و 1/57% گیاهان بررسی شده، هاپلویید بودند. در ارقام شانتال و والنتینا، تعداد 30 گیاه باززایی شده برای بررسی سطح پلوییدی انتخاب شدند و نتایج نشان داد که به ترتیب 2/40% و 5/51% آنها هاپلویید بودند. | ||
کلیدواژهها [English] | ||
آندروژنز, هاپلوئیدی, تنش اسمزی | ||
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