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Phylogenetic analysis of two Iranian grapevine virus A isolates using coat protein gene sequence | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 6، دوره 6، شماره 1 - شماره پیاپی 11، تیر 2017، صفحه 48-57 اصل مقاله (1.25 M) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2017.1373 | ||
نویسندگان | ||
Roghayeh Moradi1؛ Davoud Koolivand* 1؛ Omid Eini1؛ Mohammad Hajizadeh2 | ||
1Department of Plant Protection, Faculty of Agriculture, University of Zanjan, P. O. Box: 45371-38111, Zanjan, Iran. | ||
2Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran. | ||
تاریخ دریافت: 21 خرداد 1397، تاریخ پذیرش: 21 خرداد 1397 | ||
چکیده | ||
Symptomatic grapevine samples were collected from vineyards in Zanjan province to detect Grapevine virus A. Total RNA was extracted from symptomatic leaf samples and subjected to cDNA synthesis using random hexamer primers. Then, a DNA fragment around 800 bp including the complete coat protein (CP) gene was amplified from nine out of 57 samples by polymerase chain reaction (PCR) using specific primers. The infection rate of GAV in vineyards was around 4%, 6%, 2%, and 6% in Zanjan, Abhar, Tarom, and Khoramdareh, respectively. Two DNA fragments corresponding to samples Abhar (p25) and Zanjan (p26), were purified and sequenced. The CP-nucleotide sequence identity between two Iranian isolates was 97.3%. However, sequence identity with previously reported isolates were 76 to 95% and 82 to 98% at the nt and amino acid levels, respectively. CP-based phylogenetic trees showed three main groups (I, II, III) in which p25 (MG977013) and p26 (MG977013) isolates were placed in the group I together with isolates from different geographical regions including Palestine (Israel), Italy, Czech Republic, Jordan, USA and South Africa. To our knowledge, this is the first report of detection and phylogenetic analysis of GVA isolates from Iranian vineyards based on the complete CP gene. Positive selection value was observed on codon 25 indicating the role of this position probably in virus survival and flexibility against evolutionary forces. | ||
کلیدواژهها | ||
Coat protein؛ Phylogenetic analysis؛ RT-PCR؛ Sequencing؛ Vitivirus | ||
عنوان مقاله [English] | ||
آنالیز فیلوژنتیکی دو جدایه ویروس ای انگور با استفاده توالی ژن پروتئین پوششی | ||
نویسندگان [English] | ||
رقیه مرادی1؛ داود کولیوند1؛ امید عینی1؛ محمد حاجی زاده2 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران، کدپستی: 38111-45371. | ||
2گروه گیاهپزشکی. دانشکده کشاورزی. دانشگاه کردستان، سنندج، ایران. | ||
چکیده [English] | ||
هدف این مقاله، شناسایی ویروس ای انگور، آنالیز تنوع ژنتیکی و ارتباط فیلوژنتیکی جدایههای GVA از تاکستانهای استان زنجان است. برای این منظور، نمونهای از تاکستانهای استان زنجان بر اساس علایم مشخص جمعآوری شد. آران-ای کل از نمونه های برگ استخراج و برای ساخت cDNA آن ها از آغازگر شش تایی تصادفی استفاده شد. سپس یک قطعه دی ان ای شامل ژن کامل پروتئین پوششی ویروس در 9 نمونه از 57 نمونه بررسی شده به کمک پی سی آر، با استفاده از آغازگرهای اختصاصی تکثیر شد. دو قطعه دی ان ای تخلیص، سپس تعیین توالی شد. شباهت توالی پروتئین پوششی دو جدایه این تحقیق 97.3% در سطح نوکلئوتیدی و با سایر جدایهها -که قبلا گزارش شده بودند- 76 تا 95% در سطح اسید نوکلوئیک و 82 تا 98% در سطح اسید آمینه بود. درخت فیلوژنتیکی بر اساس ژن پروتئین پوششی، از تشکیل سه گروه (I, II, III) حکایت دارد که جدایههای ایرانی در گروه یک با جدایههایی از مناطق جغرافیایی مختلف شامل اسرائیل، ایتالیا، چک، اردن، آمریکا و آفریقای جنوبی همراه بود. بر اساس اطلاعات موجود، این اولین گزارش از ردیابی و آنالیز فیلوژنتیکی جدایههای GVA از تاکستانها براساس ژن کامل پروتئین پوششی در یکی از مناطق عمدة کشت انگور در ایران است. | ||
کلیدواژهها [English] | ||
پروتئنی پوششی, ویتی ویروس, آنالیز فیلوژنتیکی, آرتی پی سی آر, توالی یابی | ||
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