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Effect of salicylic acid on stevioside and rebaudioside A production and transcription of biosynthetic genes in in vitro culture of Stevia rebaudiana | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 1، دوره 6، شماره 2 - شماره پیاپی 12، دی 2017، صفحه 1-8 اصل مقاله (419.66 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2017.1486 | ||
| نویسندگان | ||
| Sajad Tahmasi1؛ Ghasemali Garoosi* 1؛ Jafar Ahmadi2؛ Reza Farjaminezhad1 | ||
| 1Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), P. O. Box: 34149-16818, Qazvin, Iran. | ||
| 2Department of Production an Plant Breeding, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), Qazvin, Iran. | ||
| تاریخ دریافت: 21 آبان 1397، تاریخ پذیرش: 21 آبان 1397 | ||
| چکیده | ||
| S. rebaudiana produces steviol glycosides including stevioside and rebaudioside A that are valuable as low calorie sweeteners. The objective of this study was to investigate the effects of salicylic acid elicitation and sampling times on the improvment of stevioside and rebaudioside A production and KA13H, UGT74G1 and UGT76G1 genes expression. The results showed that the addition of different concentrations of salicylic acid had different effects on stevioside and rebaudioside A production in a dosage-dependent manner. Among different concentrations of salicylic acid the highest amount of stevioside was 38.33 mg/g DW and the highest amount of rebaudioside A was 2.93 mg/g DW, observed 96 h after elicitation with 90 mg/L and 48 h after elicitation with 60 mg/L salicylic acid, respectively. Elicitation with salicylic acid increased KA13H and UGT74G1 genes expressions and decreased UTG76G1 gene expression. The correlation analysis indicated that, KA13H gene expression had a positive correlation with stevioside production. The UGT74G1 gene expression had a positive correlation with stevioside production and a negative correlation with rebaudioside A production. The UGT76G1 gene expression had a positive correlation with rebaudioside A and negative correlation with stevioside production. The qRT-PCR analysis indicated that, by increasing stevioside production under salicylic acid elicitation, the KA13H and UGT74G1 genes expression increased and UGT76G1 gene expression decreased. | ||
| کلیدواژه‌ها | ||
| Elicitation؛ Gene expression؛ Rebaudioside A؛ Stevia rebausiana؛ Stevioside | ||
| عنوان مقاله [English] | ||
| تغییرات تولید استویوزید و ربادیوزید A و بیان ژن تحت تیمار اسید سالیسالیک در کشت درون شیشه استویا | ||
| نویسندگان [English] | ||
| سجاد طهماسی1؛ قاسمعلی گروسی1؛ جعفر احمدی2؛ رضا فرجامی نژاد1 | ||
| 1گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران، کدپستی: 16818-34149. | ||
| 2گروه مهندسی ژنتیک و به‌نژادی گیاهی، دانشکده کشاورزی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران. | ||
| چکیده [English] | ||
| گیاه استویا (S. rebaudiana)، استویول گلیکوزیدهای استویوزید و ربادیوزید A را تولید میکند که به عنوان شیرینکنندههای کمکالری ارزش زیادی دارند. هدف از این مطالعه، بررسی آثار تحریک توسط اسید سالیسیلیک و زمانهای نمونهبرداری در بهبود تولید استویوزید و ربادیوزید A و بیان ژنهای KA13H، UGT74G1 و UGT76G1 است. نتایج نشان میدهد که افزودن غلظتهای مختلف اسید سالیسیلیک، بسته به مقدار استفاده از آن، آثاری متفاوت بر تولید استویوزید و ربادیوزید A دارد. بین غلظتهای مختلف اسید سالیسیلیک، بیشترین مقدار استویوزید 33/38 میلیگرم در گرم وزن خشک و ربادیوزید A 93/2 میلیگرم در گرم وزن خشک بود که به ترتیب 96 ساعت پس از تحریک با 90 میلیگرم در لیتر و 48 ساعت پس از تحریک با 60 میلیگرم در لیتر اسید سالیسیلیک به دست آمد. تحریک با اسید سالیسیلیک باعث افزایش بیان ژنهای KA13H و UGT74G1 و کاهش بیان ژن UGT76G1 شد. آنالیز همبستگی نشان داد که بیان ژن KA13H همبستگی مثبت با تولید استویوزید دارد. بیان ژن UGT74G1 همبستگی مثبت با تولید استیوزید و همبستگی منفی با تولید ربادیوزید A داشت. بیان ژن UGT76G1 نیز با تولید ربادیوزید A همبستگی مثبت و با تولید استویوزید همبستگی منفی داشته است. آنالیز qRT-PCR نشان داد که با افزایش تولید استویوزید ناشی از تحریک اسید سالیسیلیک، بیان ژنهای KA13H و UGT74G1 افزایش و بیان ژن UGT76G1 کاهش مییابد. | ||
| کلیدواژه‌ها [English] | ||
| استویوزید, الیسیتیشن, بیان ژن, ربادیوزید Stevia rebaudiana, A | ||
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