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Differences in antioxidant, morphological and biochemical responses to drought stress in different cultivars of common bean (Phaseolus vulgaris L.) | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 4، دوره 6، شماره 2 - شماره پیاپی 12، دی 2017، صفحه 36-47 اصل مقاله (951.55 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2017.1489 | ||
| نویسندگان | ||
| Kobra Saeidi1؛ Nasser Zare* 1؛ Amin Baghizadeh2؛ Rasool Asghari-Zakaria1 | ||
| 1Agronomy and Plant Breeding Department, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, P. O. Box: 56199-11367, Ardabil, Iran. | ||
| 2Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. | ||
| تاریخ دریافت: 21 آبان 1397، تاریخ پذیرش: 21 آبان 1397 | ||
| چکیده | ||
| Drought stress is the most common environmental stress that can significantly influence crop productivity. In this study, morphological and biochemical properties of 17 genotypes of common bean were evaluated under different levels of drought stress and the most sensitive and tolerant genotypes were identified using multivariate analysis. The results indicated that morphological and biochemical characteristics of common bean were significantly influenced by drought stress, genotype, and genotypes ×drought stress interaction. Principal component analysis summarized the 14 indices to four components which explained 85.71%, 84.52%, 85.86% and 84.94% of the total variation at control, moderate, severe and combined data, respectively. The correlation coefficients among most of the quantitative traits were statistically significant at all drought stress levels. A significant and positive correlation between chlorophyll and carotenoids with ascorbate peroxidase activity was observed at both moderate and severe drought stress conditions. These associations suggest that this enzyme plays an important role in ROS scavenging under drought stress. Clustering analysis grouped the genotypes into four divergent groups. The genotype-by-trait biplot analysis indicated that genotypes 1 and 2 were the most drought-tolerant and genotypes 12 and 16 were the most drought-sensitive genotypes under both moderate and severe drought stress conditions. | ||
| کلیدواژه‌ها | ||
| Anti-oxidant enzymes؛ Biplot analysis؛ Cluster analysis؛ Drought stress | ||
| عنوان مقاله [English] | ||
| پاسخ های متفاوت آنتی اکسیدانتی، مورفولوژیکی و بیوشیمیایی به تنش کمبود آب در ارقام مختلف لوبیای معمولی (Phaseolus vulgaris L.) | ||
| نویسندگان [English] | ||
| کبری سعیدی1؛ ناصر زارع1؛ امین باقی زاده2؛ رسول اصغری زکریا1 | ||
| 1گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران، کدپستی: 11367-56199. | ||
| 2گروه بیوتکنولوژی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران. | ||
| چکیده [English] | ||
| تنش کمبود آب شایعترین تنش زیست محیطی است که ممکن است بر تولید محصولات تأثیری قابل توجه برجای گذارد. در این جستار، خصوصیات مورفولوژیک و بیوشیمیایی 17 ژنوتیپ لوبیا در سطوح مختلف تنش خشکی بررسی و ارزیابی شد و ژنوتیپهای حساس و مقاوم با استفاده از تحلیل چند متغیره شناسایی شدند. نتایج پژوهش نشان میدهد که خصوصیات مورفولوژیک و بیوشیمیایی لوبیای معمولی بطور معنی داری از تنش خشکی، ژنوتیپ و تعامل ژنوتیپ و تنش خشکی متأثر شده است. بر اساس تجزیه و تحلیل اجزای اصلی، 14 شاخص در چهار جزء به ترتیب با 71/85%، 52/84%، 86/85% و 94/84% سهم، از واریانس کل در دادههای کنترل، متوسط، شدید و ترکیبی قرار گرفتند. ضرایب همبستگی بین بیشترین صفات کمّی در تمامی سطوح تنش خشکی مشاهده شد؛ چنانکه ازدیگر سو، همبستگی مثبت کلروفیل و کاروتنوئیدها با فعالیت پراکسیداز آسکورباتی در شرایط تنش خشکی متوسط و شدید نیز مشهود است. این ارتباطها نشان میدهد که این آنزیم نقشی برجسته در حذف گونههای اکسیژن فعال تحت تنش خشکی داشته است. تجزیه و تحلیل خوشهای ژنوتیپها را به چهار گروه تقسیم کرد. نتایج نمودارهای دوبعدی نشان داد که ژنوتیپهای 1 و 2 بیشترین مقاومت و ژنوتیپهای 12 و 16 کمترین مقاومت را در شرایط کمبود متوسط و شدید آب داشتند. | ||
| کلیدواژه‌ها [English] | ||
| آنزیم های آنتی اکسیدانت, تنش خشکی, خوشه بندی, نمودار دو بعدی | ||
| مراجع | ||
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