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Evaluation and characterization of ICARDAelite germplasm of lentil | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 2، دوره 7، شماره 1 - شماره پیاپی 13، تیر 2018، صفحه 9-23 اصل مقاله (634.78 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2019.9000.1199 | ||
| نویسندگان | ||
| Masoumeh Pouresmael* ؛ Mehdi Zahravi؛ Ali Akbar Ghanbari | ||
| Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), P. O. Box: 31359-33151, Karaj, Iran | ||
| تاریخ دریافت: 10 تیر 1397، تاریخ بازنگری: 20 اسفند 1397، تاریخ پذیرش: 17 بهمن 1397 | ||
| چکیده | ||
| Food legumes are a central part of the diet for many communities around the world and lentil could be an excellent choice to provide more nutritious foods. Lentil breeding program aims to develop adapted, high yielding, biotic and abiotic tolerant varieties. The success of any breeding program is dependent on the availability of genetic materials with sufficient diversity. In order to investigate the genetic diversity in elite germplasm of lentil, a total of 138 genotypes received from ICARDA were evaluated in research field of Seed and Plant Improvement Institute during 2015-2016 cropping season. These materials were studied in an observatory design by evaluating 28 quantitative and qualitative traits according to the Bioversity international descriptor. Results indicated that the highest Shannon index belonged to ground color of testa (1.33), testa pattern (0.85) and color of testa pattern (0.8). The traits seed weight per plant (CV=111.23%), grain yield (CV=81.58%) and pod weight per plant (CV=72.55%) had the highest coefficient of variation. The highest 100-seed weight and grain yield were recorded for genotypes 118 and 69, respectively. Genetic relatedness of genotypes was investigated by their pedigrees and values of genetic distance. Analysis of proximity values based on quantitative traits showed that genotypes 6 and 114 had the highest similarity and genotypes 42 and 130, had the highest genetic distance. The results of discriminant analysis of principal components indicated a successful classification based on quantitative traits in differentiating groups of genotypes. Totally, results indicate the presence of a valuable genetic diversity which could be used in advanced breeding programs. | ||
| کلیدواژه‌ها | ||
| Gene Bank؛ Genetic diversity؛ Gene pool؛ Genetic resources | ||
| عنوان مقاله [English] | ||
| ارزیابی و مشخصه‌یابی ژرمپلاسم الیت عدس | ||
| نویسندگان [English] | ||
| معصومه پوراسماعیل؛ مهدی زهراوی؛ علی اکبر قنبری | ||
| مؤسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران، کدپستی: 33151-31359 | ||
| چکیده [English] | ||
| توفیق در هر برنامة اصلاحی به سطح دسترسی مواد ژنتیکی با تنوع کافی بستگی دارد. برای بررسی تنوع ژنتیکی در ژرمپلاسم الیت عدس، 138 ژنوتیپ دریافتی از ایکاردا در مزرعة تحقیقاتی مؤسسة اصلاح و تهیه نهال و بذر در سال زارعی 1396-1397 بررسی شد. ژرمپلاسم مذکور در قالب طرح مشاهده‌ای با اندازه‌گیری 28 صفت کمّی و هشت صفت کیفی مطابق با توصیف‌نامه مرکز تحقیقات بین‌المللی بیو ورسیتی ارزیابی شد. نتایج نشان داد که بزرگترین مقدار شاخص شانون به صفات رنگ زمینة پوشش بذر (33/1)، الگوی پوشش بذر (85/0) و رنگ الگوی پوشش بذر (80/0) اختصاص داشت. صفات وزن دانه در بوته (23/111 درصد)، عملکرد دانه (58/81 درصد) و وزن غلاف در بوته (55/72 درصد) دارای بزرگترین ضریب تنوع بودند. بیشترین مقدار وزن صد دانه و عملکرد دانه به ترتیب به ژنوتیپ‌های 118 و 69 اختصاص داشت. ارتباط ژنتیکی ژنوتیپ‌ها براساس شجره و فاصله ژنتیکی آنها بررسی شد. ژنوتیپ‌های 6 و 114 دارای بیشترین شباهت و ژنوتیپ‌های 42 و 130 دارای بیشترین فاصلة ژنتیکی بودند. نتایج تجزیة تشخیصی مبتنی بر مؤلفه‌های اصلی حاکی از موفق بودن تفکیک ژنوتیپ‌ها به گروه‌های مختلف براساس صفات کمّی بود. نتایج کلی توصیف و طبقهبندی ژرمپلاسم، وجود تنوع ژنتیکی ارزشمندی را آشکار ساخت که در برنامه‌های اصلاحی پیشرفته‌تر قابل استفاده هست. | ||
| کلیدواژه‌ها [English] | ||
| خزانة ژنی, ذخایر ژنتیکی, تنوع ژنتیکی, بانک ژن | ||
| مراجع | ||
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