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ISSR Analysis for Determination of genetic diversity in 29 olive (Olea europaea L.) cultivars | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 4، دوره 7، شماره 1 - شماره پیاپی 13، تیر 2018، صفحه 31-40 اصل مقاله (1.04 M) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2019.9207.1206 | ||
نویسندگان | ||
Alireza Ghanbari* ؛ Asghar Estaji | ||
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, P. O. Box: 56199-11367, Ardebil, Iran | ||
تاریخ دریافت: 30 مرداد 1397، تاریخ بازنگری: 23 فروردین 1398، تاریخ پذیرش: 13 بهمن 1397 | ||
چکیده | ||
Olive tree is one of the most important oleaginous crops in the world, and known for having large genetic variability. Application of molecular markers is a suitable tool to investigate the genetic pool in crops. Therefore, in the current study 29 olive cultivars were tested by ten primers constituted by inter simple sequence repeats (ISSR). Plant materials were obtained from the collection of Tarom Agricultural Research Center in 2015. Genomic DNA was extracted from fresh leaves of 29 cultivars by a modified CTAB method. Ten ISSR primers were used and obtained data were scored. An analysis of primer informativeness showed that all primers were productive and effective in separating olive cultivars. Nevertheless, some of them were more effective such as UBC 855 and UBC 825 primers. According to cluster analysis and genetic distance, the olive cultivars were separated into three major clusters. Majority of the cultivars from southern and central Mediterranean such as; Italian, Spanish and Grecian olive cultivars clustered in one group. Results of the current study suggest that Mediterranean olive germplasm were structured into two main gene pools, which strongly matched two distinct geographic areas, i.e. western and central as well as eastern Mediterranean regions. | ||
کلیدواژهها | ||
Cluster analysis؛ Genetic distance؛ Inter simple sequence repeats؛ Olive | ||
عنوان مقاله [English] | ||
آنالیز ریزماهواره برای تعیین تنوع ژنتیکی 29 رقم زیتون (Olea europaea L.) | ||
نویسندگان [English] | ||
علیرضا قنبری؛ اصغر استاجی | ||
گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران، کدپستی: 11367-56199 | ||
چکیده [English] | ||
درخت زیتون (Olea europaea L.) یکی از مهمترین منابع تهیة محصولات روغنی در جهان است که دارای تنوع ژنتیکی فراوانی است. کاربرد نشانگرهای مولکولی ابزاری مفید برای پژوهش خزانة ژنتیکی محصولات هستند. به همین سبب، در این مطالعه، 29 رقم زیتون با 10 نشانگر ریزماهواره بررسی شد. نمونههای گیاهی از مرکز تحقیقات طارم در سال 1394 جمعآوری شدند. DNA ژنومی از برگهای تازة 29 قسم زیتون تغییر یافته به روش CTAB استخراج شد. در این پژوهش، به دادههای 10 آغازگر استفاده شده امتیازدهی شد. آنالیز نتایج نشانگرها نشان داد که همة آنها در جداسازی ارقام زیتون مؤثر بودهاند؛ اما برخی از آنها مانند نشانگر UBC 855، UBC 855 و UBC 825 پربازدهتر بودند. براساس نتایج تجزیة خوشهای و فاصلة ژنتیکی، ارقام زیتون در سه گروه اصلی قرار گرفتند. بیشتر ارقام مربوط به جنوب و مرکز مدیترانه؛ مانند ایتالیا، اسپانیا و یونان در یک گروه قرار گرفتند. همچنین ارقام زیتون مدیترانه براساس منطقة جغرافیایی آنها در دو گروه اصلی قرار میگیرند که شامل غرب و مرکز و همچنین شرق مدیترانه هستند. | ||
کلیدواژهها [English] | ||
زیتون, توالی های تکراری ساده, تجزیة خوشه ای, فاصلة ژنتیکی | ||
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