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Assessment of SCoT and DAMD molecular markers in genetic diversity and species delimitation of three moss species grown in Iran | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 4، دوره 7، شماره 2 - شماره پیاپی 14، دی 2018، صفحه 33-41 اصل مقاله (2.32 M) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2019.10413.1234 | ||
| نویسندگان | ||
| Somayeh Ghasemzadeh Baraki* 1؛ Sedigheh Nikzat Siahkolaee2 | ||
| 1Young Researchers and Elite Club, North Branch, Islamic Azad University, P. O. Box: 16511-53311, Tehran, Iran. | ||
| 2Young Researchers and Elite Club, North Branch, Islamic Azad University, P. O. Box: 16511-53311, Tehran, Iran | ||
| تاریخ دریافت: 24 فروردین 1398، تاریخ بازنگری: 24 تیر 1398، تاریخ پذیرش: 22 تیر 1398 | ||
| چکیده | ||
| This study was conducted to assess the efficacy of SCoT and DAMD molecular markers in genetic diversity of three moss species and compare them with ISSR marker. Another objective was to evaluate the suitability of these DNA markers in species identification (delimitation) in three moss species, including Neckera complanata, Homalothecium sericeum and Neckera crispa. To prevent ecological impact on results, all samples were collected from one locality. PIC and MI in three markers showed closely ranged values. Our results revealed that the average values of Rp and the number of species-specific bands in SCoT primers were more than those of DAMD and ISSR. It showed considerable capability of SCoT marker in discriminating individuals. The highest value of genetic parameters Na (1.08), He (0.15) and I (0.23) were obtained with SCoT marker showing the power of this marker in genetic diversity analysis. Moreover, dendrograms produced from SCoT and DAMD data indicated similar results in the placement of closely related species. SCoT markers were shown to be more efficient than DAMD and ISSR markers based on the multiplex ratio (EMR), Rp, genetic diversity parameters (Na, He, I) and the number of species-specific bands. The findings demonstrated that the SCoT and DAMD markers could be applied for the estimation of genetic relationships and separation of two closely related genera. This is the first report of its type on the genetic diversity of mosses by application of SCoT and DAMD. | ||
| کلیدواژه‌ها | ||
| DAMD؛ Efficiency؛ Genetic diversity؛ Moss؛ SCoT | ||
| عنوان مقاله [English] | ||
| ارزیابی کارایی نشانگرهای مولکولی DAMD وSCoT در مطالعات تنوع ژنتیکی و تعیین حدود گونه ها در برخی خزه ها در ایران | ||
| نویسندگان [English] | ||
| سمیه قاسم زاده برکی1؛ صدیقه نیک ذات سیاهکلایی2 | ||
| 1گروه ژنتیک، باشگاه پژوهشگران جوان، دانشگاه آزاد اسلامی، واحد تهران شمال، ایران، کدپستی: 16511-53311. | ||
| 2گروه زیست شناسی، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران. | ||
| چکیده [English] | ||
| پژوهش حاضر برای بررسی و مقایسه کارایی نشانگرهای مولکولی SCoT و DAMD در سنجش میزان تنوع ژنتیکی و همچنین مقایسة نتایج حاصل از آنها با نشانگر ISSR انجام گرفته است. یکی از اهداف این مطالعه تعیین کارایی این روش‌ها در میزان پلی مورفیسم DNA و دیگر پارامترهای تنوع ژنتیکی بود. هدف دیگر از این مطالعه ارزیابی کارایی این سه روش در تعیین حد و مرز گونه‌های Neckera complanata, Homalothecium sericeum ،Neckera crispa بود. برای جلوگیری از تأثیر عوامل اکولوژیکی نمونه‌هایی از یک منطقه جمع آوری شد. میزانPIC و MI در نشانگرهای مورد مطالعه نشان دهنده مقادیر مشابهی است. نتایج نشان داد که متوسط میزان Rp و تعداد باندهای مختص گونه‌‌ای پرایمرهای SCoT نسبت به DAMD و ISSR کارایی بهتری در جدا کردن گونه‌ها دارند. بیشترین میزان پارامترهای تنوع ژنتیکی برای Na ، 08/1، He، 15/0 و I 23/0 بود که از نشانگر SCoT به دست آمد که توانایی زیاد نشانگر SCoT را در مطالعة تنوع ژنتیکی نشان می‌دهد. همچنین درختچه حاصله از دو نشانگر SCoT و DAMD مشابهت بسیاری در جایگیری گونه‌های نزدیک داشته است. بر اساس مقادیر پارامترهای EMR، Rp، پارامترهای تنوع ژنتیکی (Na، He و I) و تعداد باندهای اختصاصی، نشانگر SCoT کارآمدتر از نشانگرهای DAMD و ISSR عمل کرده است. یافته‌ها نشان داد که نشانگرهای DAMD و SCoT در تخمین روابط ژنتیکی به خوبی استفاده شده و جدایی دو جنس نزدیک به هم را نشان داده است. به کارگیری دو نشانگر مولکولی SCoT و DAMD برای بررسی تنوع ژنتیکی در خزه‌ها در این تحقیق برای اولین بار در دنیا صورت گرفته است. | ||
| کلیدواژه‌ها [English] | ||
| تنوع ژنتیکی, خزه, کارایی, نشانگرDAMD, نشانگر SCoT | ||
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