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Genetic diversity among tall fescue ecotypes using agro-morphological traits, nutritional values and AFLP molecular markers | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 6، دوره 7، شماره 2 - شماره پیاپی 14، دی 2018، صفحه 54-64 اصل مقاله (530.92 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2019.10480.1236 | ||
| نویسندگان | ||
| Fatemeh Amini* ؛ Peyman Masoomi؛ Hossein Ramshini؛ Mohamad Ali Norouzian | ||
| Department of Agronomy and Plant Breeding Sciences, College of Abouraihan, University of Tehran, P. O. Box: 33916-53755, Tehran, Iran. | ||
| تاریخ دریافت: 07 اردیبهشت 1398، تاریخ بازنگری: 08 مرداد 1398، تاریخ پذیرش: 27 شهریور 1398 | ||
| چکیده | ||
| This study was carried out to compare agro-morphological traits and nutritive value of 25 tall fescue (Festuca arundinacea) ecotypes grown in Tehran, Iran. The experiments were carried out in a split plot design during 2016-2018 growing seasons. The results showed that there was a significant genetic variation in existing germplasm. The highest heritability was related to dry forage yield and collar diameter (88%). The low heritability levels of forage quality related traits indicated that environmental effects play a greater role in controlling this trait. The mean comparison showed that Isfahan ecotype 11 had the highest number of fertile shoots, dry forage yield and diameter of collar while ecotypes of Shahrood ecotype 9 had the highest percentage of dry mater and Dauphine genotype with 16.05% had the highest protein percentage. The number of fertile shoots (NFS) had a positive and significant correlation with dry forage yield (DFY) and plant height (PH). DFY was positively correlated with NDF (0.71) and negatively correlated with CP (-0.62). In AFLP analysis out of the 463 scored bands, 339 (68%) were polymorphic. PIC values ranged from 0.34 (EcoACA-MseCTA) to 0.10 (EcoAGC-MseCAC). The results showed that genetic distances between ecotypes based on agro-morphologic characters and nutritional values were correlated based on AFLP (r=0.41, P=0.05) results. Based on cluster analysis all genotypes were classified into 4 genotypic groups. Considerable genetic variation and high heritability estimates indicate that direct selection for increasing forage yield could be promising. However, breeding nutritional values of forage quality might be more difficult due to environmental effects. | ||
| کلیدواژه‌ها | ||
| Cluster analysis؛ Forage yield؛ Forage quality؛ Heritability | ||
| عنوان مقاله [English] | ||
| بررسی تنوع ژنتیکی فسکیوی بلند ایرانی با استفاده از صفات مورفولوژی و زراعی، ارزش غذایی و نشانگر مولکولی AFLP | ||
| نویسندگان [English] | ||
| فاطمه امینی؛ پیمان معصومی؛ حسین رامشینی؛ محمدعلی نوروزیان | ||
| گروه علوم زراعی و اصلاح نباتات، پردیس ابوریحان، دانشگاه تهران، ایران، کدپستی: 53755-33916. | ||
| چکیده [English] | ||
| پژوهش پیشِ‌رو برای مطالعة صفات مورفولوژی زراعی و ارزش تغذیه‌ای 25 اکوتیپ فسکیوی بلند(Festuca arundinacea) در قالب طرح اسپیلیت پلات در زمان، طی سال‌های زراعی 97-95 ایران اجرا شد. نتایج نشان داد تنوع ژنتیکی معنی‌داری در ژرم پلاسم مورد مطالعه وجود داشت. بیشترین وراثت‌پذیری مربوط به صفت عملکرد علوفه خشک و قطر یقه (88%) بود. کمترین مقادیر وراثت‌پذیری در صفات مرتبط با کیفیت علوفه نشان‌دهنده نقش آثار محیطی در کنترل این صفات بود. مقایسه میانگین صفات نشان داد، اکوتیپ شمارة 11 از اصفهان دارای بیشترین تعداد شاخة بارور و عملکرد علوفه خشک و قطر یقه بود؛ در حالی‌که اکوتیپ شماره 9 از شاهرود بیشترین درصد مادّه خشک و ژنوتیپ دافین با 16 درصد پروتئین، دارای بیشترین میزان این صفات بودند. همبستگی صفات تعداد شاخه بارور با عملکرد علوفه خشک و ارتفاع گیاه، مثبت و معنی‌دار بود. همچنین همبستگی عملکرد علوفه خشک با صفت مقدار فیبر غیرمحلول (NDF)، مثبت و معنی‌دار (77/0) و با مقدار پروتئین منفی و معنی‌دار (62/0-) بود. در الگوی نواری نشانگرهای مولکولی AFLP، 339 باند از مجموع 463 باند(68%) چند شکلی نشان دادند. محتوای اطلاعات چند شکلی نیزدارای مقادیر 34/0 برای ترکیب آغازگری (EcoACA-MseCTA) تا 1/0 برای ترکیب آغازگری ((EcoAGC-MseCAC بودند. نتایج نشان داد فاصله ژنتیکی بین اکوتیپ‌ها بر اساس صفات مورفولوژیک، زراعی و ارزش غذایی با فاصله ژنتیکی بین اکوتیپ‌ها بر اساس داده‌های AFLP مثبت و معنی‌دار (r=0.41, P = 0.05) بود. تجزیه خوشه‌ای به روش وارد بر اساس ماتریس مربع فاصله اقلیدسی نمونه‌ها را به چهار گروه طبقه بندی کرد. تنوع ژنتیکی و وراثت پذیری زیاد نشان داد، انتخاب مستقیم برای بهبود صفت عملکرد علوفه سودمند خواهد بود. با این حال اصلاح صفات مرتبط با ارزش غذایی به علت آثار محیطی دشوارتر خواهد بود. | ||
| کلیدواژه‌ها [English] | ||
| تجزیه خوشه‌ای, عملکرد علوفه, کیفیت علوفه, وراثت پذیری | ||
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