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Effect of total nitrogen content and NH4+/NO3- ratio on biomass accumulation and secondary metabolite production in cell culture of Salvia nemorosa | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 3، دوره 9، شماره 1 - شماره پیاپی 17، تیر 2020، صفحه 17-27 اصل مقاله (623.81 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2020.12321.1258 | ||
نویسندگان | ||
Hamid Reza Heydari؛ Esmaeil Chamani* ؛ Behrooz Esmaeilpour | ||
Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, P. O. Box: 56199-11367, Ardabil, Iran. | ||
تاریخ دریافت: 07 دی 1398، تاریخ بازنگری: 31 فروردین 1399، تاریخ پذیرش: 17 اردیبهشت 1399 | ||
چکیده | ||
Woodland sage (Salvia nemorosa) is an important native medicinal plant in Iran and is considered as a rich source of phenolic and flavonoids compounds. The present research was conducted with respect to the optimization of medium for biomass accumulation and secondary metabolite production in cell suspension culture of S. nemorosa. In this research the effects of total nitrogen content (0, 15, 30, 60, 90, and 120 mM) and NH4+/NO3- ratio (0:60, 10:50, 20:40, 30:30, 40:20, 50:10, and 60:0) were studied in the Murashige and Skoog Medium on biomass growth, total phenolic, flavonoid and rosmarinic acid contents. The maximum accumulation of fresh biomass (294.80 g/l) and total phenolic content (76.61 mg GAE/g DW) was obtained in the medium supplemented with 90 mM nitrogen. The highest rosmarinic acid content (16.41 and 16.16 mg/g DW) was recorded in the medium containing 30 and 60 mM nitrogen. Increasing total nitrogen above 30 mM resulted in a decline in rosmarinic acid production. The ammonium to nitrate ratio also affected the biomass growth and secondary products accumulation. The highest fresh biomass accumulation (296.52 g/l), total phenolic content (87.30 mg GAE/g DW) and Rosmarinic acid content (18.43 mg/ DW) were recorded in 10:50 ratio of NH4+/NO3-. Increasing the NH4+ level or complete elimination of it from culture medium reduced the rosmarinic acid and total phenolic content of S. nemorosa. Our findings revealed that Woodland sage cell suspension needs both nitrogen forms, but ammonium is required at low concentration and nitrate at high levels. The results of the current study are beneficial for medium optimization for the establishment of large scale and bioreactor assisted cell culture of woodland sage for the production of phenolic acids. | ||
کلیدواژهها | ||
Ammonium؛ Nitrate؛ Rosmarinic acid؛ Suspension culture؛ Woodland sage | ||
عنوان مقاله [English] | ||
تاثیر نیتروژن کل و نسبت آمونیوم به نیترات بر رشد زیست توده و تولید متابولیت های ثانویه در کشت سلولی مریم گلی مزرعه ای | ||
نویسندگان [English] | ||
حمیدرضا حیدری؛ اسماعیل چمنی؛ بهروز اسماعیل پور | ||
گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران، کد پستی: 56199-11367. | ||
چکیده [English] | ||
مریم گلی مزرعه ای یکی از مهم ترین گیاهان بومی در ایران می باشد که به عنوان یک منبع غنی ترکیبات فنولی و فلاوونوئیدی شناخته می شود. پژوهش حاضر به منظور بهینه سازی محیط کشت برای رشد زیست توده و تولید متابولیت های ثانویه در کشت سوسپانسیون سلولی مریم گلی مزرعه ای انجام گرفت. در این پژوهش اثر غلظت نیتروژن کل (0، 15، 30، 60، 90 و 120 میلی مولار) نیتروژن کل و نسبت های مختلف آمونیوم به نیترات ( 0:60، 10:50، 20:40، 30:30، 40:20، 50:10 و 60:0 میلی مولار) در محیط کشت MS بر شاخص های رشد زیست توده، فنول کل، فلاونوئید کل و محتوای رزمارینیک اسید بررسی شد. بیشترین میزان وزن تر (80/294 گرم بر لیتر) و فنول کل (GAE/g DW 60/76) در محیط حاوی 90 میلی مولار نیتروژن به دست آمد. بالاترین میزان رزمارینیک اسید (41/16 و 16/16 میلی گرم بر گرم وزن خشک) در محیط های حاوی 30 و 60 میلی مولار نیتروژن ثبت شد. افزایش نیتروژن کل به میزان بالاتر از 30 میلی مولار، منجر به کاهش تولید رزمارینیک اسید شد. همچنین نسبت آمونیوم به نیترات رشد زیست توده و تجمع متابولیت های ثانویه را تحت تاثیر قرار داد. بیشترین میزان وزن تر (74/267 گرم بر لیتر)، فنول کل (GAE/g DW 30/87) و محتوای رزمارینیک اسید (43/18 میلی گرم بر لیتر) در محیط حاوی نسبت 50:10 آمونیوم به نیترات مشاهده گردید. افزایش غلظت آمونیوم یا حذف کامل آن از محیط کشت سبب کاهش میزان فنول کل و رزمارینیک اسید در کشت سلولی مریم گلی مزرعه ای می شود. | ||
کلیدواژهها [English] | ||
آمونیوم, رزمارینیک اسید, کشت سوسپانسیون, مریم گلی مزرعه ای, نیترات | ||
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