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Molecular study of mollusca in Bandar Lengeh using 18S rRNA gene | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 11، دوره 8، شماره 2 - شماره پیاپی 16، مرداد 2019، صفحه 57-65 اصل مقاله (638.21 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2020.12880.1267 | ||
| نویسندگان | ||
| Raheem Haddad* 1؛ Esmat Alemzadeh2، 3؛ Alireza Ahmadi4؛ Mohammad Sediq Mortazavi5؛ Maryam Moezzi5؛ Effat Alemzadeh6؛ Saeid Tamadoni Jahromi5 | ||
| 1Biotechnology Department, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, P. O. Box. 34149-16818, Qazvin, Iran | ||
| 2Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran | ||
| 3Department of Medical Biotechnology, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran | ||
| 4Department of Biomedical Sciences, Alzahra University, Tehran, Iran | ||
| 5Persian Gulf & Oman Sea Ecological Research Institute, Bandar Abbas, Iran | ||
| 6Postdoc Position in Parasitology, Birjand University of Medical Sciences, Birjand, Iran | ||
| تاریخ دریافت: 21 اسفند 1398، تاریخ بازنگری: 31 اردیبهشت 1399، تاریخ پذیرش: 07 اردیبهشت 1399 | ||
| چکیده | ||
| Mollusca are one of the most diverse animal phyla whose phylogeny is considered as a controversial subject. Although some groups were traditionally classified as mollusca, they need to be identified again. High diversity of mollusca has created considerable taxonomic problems and despite their importance in marine biota, deep phylogenetic relationships of mollusca have scarcely been investigated. The aim of this study was to determine genetic diversity and differences between mollusca species in waters of Bandar Lengeh (Persian Gulf) of Iran. A clone library of the ribosomal small subunit RNA gene (18S rDNA) in the nuclear genome was constructed by PCR, and then, after examining the clones, selected clones were sequenced. The determined clone sequences were analyzed by a similarity search of the NCBI GenBank database using BLAST. Also, the fixation index (FST) factor was used in order to measure and understand the genetic differences between studied populations. In this study, seventeen sequences were identified belonging to two classes of Bivalvia and Gastropoda and they were used for phylogenetic analysis. According to the allele frequencies at each locus, FST value was significant in samples of Bandar Lengeh meaning that migration is in the lowest rate in the studied region. The present research project exhibited that clone library of 18S rDNA might be accounted as a beneficial tool to identify marine specimens and estimate the actual species diversity in marine environments. Moreover, these findings confirmed the effective role of molecular studies for the identification and taxonomy of speciesfrom the natural resources to obtain reliable data. | ||
| کلیدواژه‌ها | ||
| 18S rDNA؛ Clone library؛ Genetic diversity؛ Mollusca؛ Persian Gulf | ||
| عنوان مقاله [English] | ||
| مطالعه ی مولکولی نرم تنان بندر لنگه با استفاده از ژن 18S rRNA | ||
| نویسندگان [English] | ||
| رحیم حداد1؛ عصمت عالم زاده2، 3؛ علیرضا احمدی4؛ محمد صدیق مرتضوی5؛ مریم معزی5؛ عفت عالم زاده6؛ سعید تمدنی جهرمی5 | ||
| 1گروه بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران، کدپستی: 34149-16818. | ||
| 2مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی بیرجند، بیرجند، ایران. | گروه بیوتکنولوژی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی بیرجند، بیرجند، ایران. | ||
| 3مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی بیرجند، بیرجند، ایران. | گروه بیوتکنولوژی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی بیرجند، بیرجند، ایران. | ||
| 4گروه بیومدیکال، دانشگاه الزهرا، تهران، ایران. | ||
| 5پژوهشکده اکولوژی خلیج فارس و دریای عمان، بندرعباس، ایران. | ||
| 6پسادکتری در رشته انگل شناسی، دانشگاه علوم پزشکی بیرجند، بیرجند، ایران. | ||
| چکیده [English] | ||
| تنوع بالای نرم تنان باعث مشکلات عدیده ی تاکسونومی میگردد و جدای از اهمیت آن‌ها در زیست بوم دریایی، روابط عمیق فیلوژنتیک آن‌ها بندرت مورد بررسی قرار گرفته است. هدف از این مطالعه تعیین تنوع ژنتیکی و تفاوت های گونه های نرم تنان خلیج فارس در ایران می باشد. کتابخانه ی کلونی ژن RNA زیر واحد کوچک ریبوزومی (18S rDNA) در ژنوم هسته ای با استفاده از PCR ساخته شد و پس از بررسی و آزمایش، کلون های منتخب توالی یابی شدند. توالی های کلون تعیین شده بوسیله ی جستجوی مشابهت در پایگاه داده ی NCBI GenBank با استفاده از BLAST مورد آنالیز قرار گرفتند. همچنین از فاکتور FST برای اندازه گیری و درک اختلافات ژنتیکی مابین جمعیت های مورد مطالعه استفاده شد. در این مطالعه هفده توالی شناسایی گردید که به دو کلاس Bivalvia و Gastropoda تعلق داشتند و برای آنالیز فیلوژنتیکی مورد استفاده قرار گرفتند. براساس فراوانی های آللی در هر لوکوس، مقدار FST در نمونه های خلیج فارس در مقایسه با نمونه های NCBI معنی دار بود. این بدین معنی است که نرخ مهاجرت در دو منطقه ی مورد مطالعه در پایین ترین حد خود بود. براساس تست های ANOVA مقدار فاصله ی ژنتیکی بین دو ناحیه 60/0 بود که مقدار قابل ملاحظه ای است. این یافته ها نقش مهمی در مطالعات روابط بین جمعیت ها و ذخایر آبی داشته و بیانگر کاربرد مطالعات مولکولی بعنوان ابزاری موثر در شناسایی و تاکسونومی گونه های موجود در منابع طبیعی جهت دستیابی به داده های قابل اعتماد می باشد. | ||
| کلیدواژه‌ها [English] | ||
| 18S rDNA, کتابخانه ی کلونی, تنوع ژنتیکی, نرم تنان, خلیج فارس | ||
| مراجع | ||
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