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Effective in vitro seed germination and direct regeneration from cotyledonary leaf explants of Nitraria schoberi | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 2، دوره 9، شماره 1 - شماره پیاپی 17، تیر 2020، صفحه 10-16 اصل مقاله (506.12 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2020.11945.1255 | ||
| نویسندگان | ||
| Samaneh Kheirabadi1؛ Nasim Zarinpanjeh* 2؛ Mohammad Ali Ebrahimi1؛ Golamreza Bakhshi Khaniki1؛ Hamid Reza Naseri3 | ||
| 1Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran. | ||
| 2Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, P. O. Box: 31375-1369, Karaj, Iran. | ||
| 3Department of Desert Management, International Desert Research Center (IDRC), University of Tehran, Tehran, Iran. | ||
| تاریخ دریافت: 06 آذر 1398، تاریخ بازنگری: 31 خرداد 1399، تاریخ پذیرش: 28 تیر 1399 | ||
| چکیده | ||
| Nitraria schoberi (Ghar-e-Daghin in Persian) from Zygophyllaceae (Nitrariaceae) family is considered as a wonderful exquisite plant in terms of its economical, medicinal, animal feed, significant values and also its unique characteristics of being tolerant to the soil salinity, drought and erosion. On the other hand, this plant is challenging with severe problems such as seed dormancy, plant growth inhibitors and also hard seed coat which lead to retard germination and subsequently to reduce the chance of life and reproduction. The present study was conducted for the first time to cope with its seed germination obstacles and to induce plantlet regeneration in N. schoberi through tissue culture method. Effect of cotyledonary leaf and hypocotyl explants and various concentrations of plant growth regulators were studied on shoot and root induction and further development. Based on the obtained results, culturing seeds after removing its seed coat effectively improved germination rate (80%) within a week. Whereas, germination of seeds with seed coats required a longer time (4 weeks) and showed a sharp decline in percentage (10%). At the next step, culturing cotyledonary leaf explants on shoot induction media including MS medium supplemented with BAP (2.0 mgL-1) and IBA (0.5 mgL-1) gave the highest shoot regeneration rate (93.33%), with average shoot number per explant (3.67) and shoot length (4.6 cm) after 60 days. Hypocotyl explants showed no response to shoot induction treatments even after 60 days. Regenerated shoots were rooted on the MS medium supplemented with IAA (1.0 mgL-1) alone or in combination with IBA (2.0 mgL-1). Finally, all regenerated plantlets were acclimatized and transferred to the green house successfully with 70% survival rate. | ||
| کلیدواژه‌ها | ||
| Nitraria schoberi؛ In vitro culture؛ Seed germination؛ Direct regeneration؛ Plant growth regulators | ||
| عنوان مقاله [English] | ||
| معرفی روشی کار آمد در جوانه زنی درون شیشه ای و ریزادیادی مستقیم از ریزنمونه های برگ های کوتیلدونی گیاه قره داغ ( Nitraria schoberi) | ||
| نویسندگان [English] | ||
| سمانه خیرآبادی1؛ نسیم زرین پنجه2؛ محمدعلی ابراهیمی1؛ غلامرضا بخشی خانیکی1؛ حمیدرضا ناصری3 | ||
| 1گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران. | ||
| 2مرکز تحقیقات گیاهان دارویی، کرج، ایران، کد پستی: 31375-1369. | ||
| 3گروه مدیریت بیابان، مرکز تحقیقات بین المللی بیابان (IDRC)، دانشگاه تهران ، تهران ، ایران. | ||
| چکیده [English] | ||
| گیاه قره داغ با نام علمی Nitraria schoberi از خانواده Zygophyllaceae Nitrariaceae است. این گیاه به دلیل دارا بودن ارزشهای اقتصادی، دارویی، خوراک دام و همچنین ویژگیهای منحصر به فرد آن برای تحمل شوری خاک، خشکسالی و فرسایش بسیار مهم و ارزشمند است. به عبارت دیگر، این گیاه در زمینه هایی مانند خواب بذر، بازدارنده های رشد گیاهی و پوسته سخت بذر، مشکلاتی دارد. این چالشها منجر به تأخیر در جوانه زنی و به دنبال آن کاهش شانس زنده ماندن و تولید مثل در این گیاه میگردد. تحقیق حاضر برای اولین بار برای مقابله با موانع جوانه زنی بذر و القا باززایی گیاه قره داغ از طریق روش کشت بافت انجام گرفت. برگهای کوتیلدونی و هیپوکوتیل به عنوان ریزنمونه و همینطور غلظتهای مختلفی از تنظیم کننده های رشد گیاهی برای القا و رشد ساقه و ریشه بررسی شدند. بر اساس نتایج به دست آمده، کشت بذرها بعد از حذف پوستة آن، جوانه زنی مؤثری (80 درصدی) را طی یک هفته از خود نشان داد. درحالیکه بذرهای کشت شده همراه با پوسته بذر پس از گذشت چهار هفته تنها 10 درصد جوانه زنی نشان دادند. در مرحله بعد، کشت ریزنمونه برگ کوتیلدونی در محیط کشت القا ساقه شامل محیط کشت MS حاویBAP (0/2 میلیگرم درلیتر) و IBA (0/5 میلیگرم در لیتر) به عنوان تنظیم کننده های رشد گیاهی، بیشترین فراوانی باززایی (33/99%)، تعداد نوساقه باززاشده در هر ریزنونه (67/3) و طول نوساقه (6/4 سانتی متر) را پس از گذشت 60 روز نشان داد. درحالی که ریزنمونه های هیپوکوتیل حتی پس از گذشت 60 روز نیز هیچ واکنشی در محیطهای کشت القا نوساقه از خود نشان ندادند. نوساقه های باززا شده در محیط کشت القای ریشه شامل محیط کشت MS حاوی IAA (0/1 میلی گرم درلیتر) به تنهایی و یا درترکیب باIBA (0/2 میلی گرم درلیتر ) به خوبی ریشهدار شدند. گیاهچه های باززایی شده درمرحله بعد به خاک منتقل و درگلخانه نگهداری شدند. بدین ترتیب، 70 درصد گیاهچه ها پس از سازگاری زنده ماندند | ||
| کلیدواژه‌ها [English] | ||
| گیاه قره داغ, کشت درون شیشه ای, جوانه بذر, باززایی مستقیم, تنظیم کننده های رشد گیاهی | ||
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