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Classification of high and low molecular weight glutenin subunits and related genes in tetra-hexaploid wheat landraces | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 9، شماره 1 - شماره پیاپی 17، تیر 2020، صفحه 51-60 اصل مقاله (607.58 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2020.13805.1279 | ||
| نویسندگان | ||
| Abolfazl Bozorgmehr1؛ Jafar Ahmadi* 1؛ Fahimeh Shahinnia2؛ Khadijeh Razavi3؛ Goodarz Najafian4؛ Tahmineh Lohrasebi3 | ||
| 1Department of Genetics and Plant Breeding, Imam Khomeini International University, P. O. Box: 34148-96818, Qazvin, Iran. | ||
| 2Leibniz-Institute for Plant Genetics and Crop Plant Research, Germany. | ||
| 3National Institute for Genetic Engineering and Biotechnology, Tehran, Iran. | ||
| 4Seed and Plant Improvement Institute, Karaj, Iran. | ||
| تاریخ دریافت: 01 مرداد 1399، تاریخ بازنگری: 05 مهر 1399، تاریخ پذیرش: 12 مهر 1399 | ||
| چکیده | ||
| Wheat is the first and the most important grain of the world and its bakery property depends on gluten quality. Gluten is a part of endosperm hard proteins that causes increased stickiness quality. Wheat glutenin is divided into two groups according to their molecular weight, glutenin subunits with high molecular weight (HMW-GS) and glutenin subunits with low molecular weight (LMW-GS). In the present study, 97 Iranian wheat landraces were analyzed for diversity in high and low molecular weight glutenin subunits. In hexaploids, 11 different high molecular weight subunits were identified, three of which were related to Glu-A1, five for Glu-B1 and three were related to Glu-D1. Interestingly, the subunit 2.1+10* was observed in three landraces.In tetraploids, nine subunits were identified, of which two were related to Glu-A1 and seven were related to Glu-B1. Low molecular weight glutenin subunit encoding genes were investigated using seven DNA primer pairs for Glu-A3, Glu-B3 and Glu-D3 loci. Five alleles were identified for Glu3-A.2 with frequencies ranging from 3.092 to 96.907.Two alleles were identified by Glu3-A3 with relative frequencies of 0.412 and 0.608%.For Glu3-B.1, 10 alleles were identified, with frequencies ranging from 1.030 to 92.783. However, three alleles a, b and c were identified by Glu3-B.2 with frequencies of 0.01, 0.78 and 0.20, respectively. Three specific primers were used for Glu-D3 locus and because durum wheat lacks DD genome, it was not estimated in this genetic block. Using PCR amplification, nine alleles were identified for Glu3-D1 with frequencies ranging from 5.34 to 86.67. Also two and three alleles were identified using Glu3-D.3 and Glu3-D.4, respectively. | ||
| کلیدواژه‌ها | ||
| Classification؛ Glutenin؛ HMW-GS؛ LMW-GS | ||
| عنوان مقاله [English] | ||
| طبقه بندی زیرواحدها و ژن های گلوتنین با وزن مولکولی بالا (HMW-GS) و پایین (LMW-GS) در ژنوم گندم تترا-هگزاپلوئید | ||
| نویسندگان [English] | ||
| ابوالفضل بزرگمهر1؛ جعفر احمدی1؛ فهیمه شاهین نیا2؛ خدیجه رضوی3؛ گودرز نجفیان4؛ تهمینه لهراسبی3 | ||
| 1گروه ژنتیک و به‌نژادی گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران، کدپستی: 34148-96818. | ||
| 2موسسه لایب نیتس برای تحقیقات ژنتیکی گیاهی و تحقیقات گیاهان زراعی ، آلمان. | ||
| 3پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران. | ||
| 4موسسه بهبود بذر و نهال، کرج، ایران. | ||
| چکیده [English] | ||
| گندم اولین و مهمترین محصول غله‌ای در جهان است و خاصیت نانوایی آن به کیفیت گلوتن بستگی دارد. گلوتن بخشی از پروتئین های آندوسپرم است که باعث افزایش کیفیت چسبندگی می‌شود. گلوتنین گندم با توجه به وزن مولکولی آنها، به دو گروه زیر واحد گلوتنین با وزن مولکولی زیاد (HMW-GS) و زیر واحد گلوتنین با وزن مولکولی کم (LMW-GS) تقسیم می‌شوند. در مطالعة حاضر، 97 توده بومی گندم ایران از نظر تنوع در زیر واحدهای گلوتنین با وزن مولکولی زیاد و اندک تجزیه و تحلیل شد. در هگزاپلوئیدها، 11 زیر واحد مولکولی مختلف شناسایی شد که سه مورد مربوط به Glu-A1، پنج مورد مربوط به Glu-B1 و سه مورد مربوط به Glu-D1 بود. نکتة مهم مشاهدة زیر واحد *10 + 1/2 در سه توده بومی بود. در تتراپلوئیدها، نه زیر واحد شناسایی شد که دو زیرواحد مربوط به Glu-A1 و هفت زیرواحد مربوط به Glu-B1 بود. ژن‌های کد کننده زیر واحد گلوتنین با وزن مولکولی کم با استفاده از هفت جفت آغازگر DNA برای مکان‌های Glu-A3 ، Glu-B3 و Glu-D3 مورد بررسی قرار گرفتند. پنج آلل توسط Glu3-A.2 شناسایی شد که دارای فراوانی‌های 092/3 و 907/96 بودند. دو آلل توسط Glu3-A3 با فراوانی‌های نسبی 412/0 و 608/0 شناسایی شدند. Glu3-B.1 نیز 10 آلل را شناسایی کرد که فراوانی آنها از 03/1 تا 78/92 بود. با این حال، سه آلل a ، b و c توسط Glu3-B.2 به ترتیب با فراوانی‌های 01/0 ، 78/0 و 20/0 شناسایی شدند. از سه آغازگر اختصاصی برای مکان Glu-D3 استفاده شد و از آنجا که گندم دوروم فاقد ژنوم DD است، در این بلوک ژنتیکی تخمین آللی زده نشد. با استفاده از واکنشPCR ، نه آلل برای Glu3-D1 با فراوانی‌های 34/5 تا 67/86 شناسایی شد. همچنین دو و سه آلل به ترتیب با استفاده از Glu3-D.3 و Glu3-D.4 شناسایی شدند. | ||
| کلیدواژه‌ها [English] | ||
| طبقه بندی, گلوتنین, HMW-GS, LMW-GS | ||
| مراجع | ||
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