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Expression analysis of lipid transfer protein gene in wheat (Triticum aestivum) under drought stress | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 9، شماره 1 - شماره پیاپی 17، تیر 2020، صفحه 96-103 اصل مقاله (531.53 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2021.13622.1274 | ||
| نویسندگان | ||
| Sedigheh Nikaeen1؛ Mohammad Mehdi Yaghoobi1؛ Ali Niazi* 2؛ Ali Moghadam2؛ Mohammad Sadegh Taghizadeh2 | ||
| 1Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. | ||
| 2Institute of Biotechnology, Shiraz University, P. O. Box: 65186-71441, Shiraz, Iran. | ||
| تاریخ دریافت: 31 تیر 1399، تاریخ بازنگری: 20 بهمن 1399، تاریخ پذیرش: 28 بهمن 1399 | ||
| چکیده | ||
| Drought stress causes changes in morphology, physiology and gene expression profile in the plants. One of the ways to respond to this stress is to change the synthesis of specific polypeptides such as LTP (Lipid transfer proteins). For this purpose, LTp expression level was investigated under three osmotic potentials of -2, -4 and -6 bar in combination with different time courses of 0, 3, 6, 10, 24, 48 and 72 h after applying stress using RT-qPCR in DN-11 and Marvdasht genotypes. Also, the soluble sugar content in both genotypes was measured by the phenol-sulfuric acid method after each stress level. Furthermore, promoter analysis of LTp was studied using bioinformatics tools. The results showed that the highest expression level of LTp in both genotypes occurred at -6 bar osmotic potential level and 48 h after stress in DN-11 and 72 h after stress in Marvdasht. There was no significant difference between 48 h and 72 h after stress in the DN-11 genotype and between 72 h and 3 h after stress in Marvdasht genotype at P-value of ≤ 0.01, but there was a significant difference among other time courses at P-value of ≤ 0.01. Besides, the soluble sugar content increased with increasing stress levels in both genotypes, so that its amount was higher than control at -6 bar stress level. The promoter analysis showed that several domains and motifs in the LTp promoter region are activated in response to drought stress and increase its expression. Therefore, it can be concluded that LTp gene can be used as a drought resistance gene in gene transformation and genetic engineering programs. | ||
| کلیدواژه‌ها | ||
| Drought stress؛ Gene expression analysis؛ LTP؛ RT-qPCR؛ Triticum aestivum | ||
| عنوان مقاله [English] | ||
| بررسی بیان ژن LTP در گندم (Triticum aestivum) تحت تنش خشکی | ||
| نویسندگان [English] | ||
| صدیقه نیک آیین1؛ محمد مهدی یعقوبی1؛ علی نیازی2؛ علی مقدم2؛ محمد صادق تقی زاده2 | ||
| 1گروه بیوتکنولوژی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه صنعتی تحصیلات تکمیلی، کرمان، ایران. | ||
| 2پژوهشکده بیوتکنولوژی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران، کد پستی: 65186-71441. | ||
| چکیده [English] | ||
| تنش خشکی سبب ایجاد تغییرات مورفولوژی، فیزیولوژی و پروفایل بیان ژن در گیاهان می‌شود. یکی از راه‌های پاسخ به این تنش، تغییر سنتز پلی پپتیدهای اختصاصی به نام « LTP» یعنی پروتئین‌های انتقال دهندة لیپید است. در این مطالعه، سطح بیان LTP تحت سه پتانسیل اسمزی 2- ، 4- و 6- بار در ترکیب با زمان‌های مختلف 0، 3، 6، 10، 24، 48 و 72 ساعت پس از تنش با استفاده از RT-qPCR در ژنوتیپ‌های DN-11 و Marvdasht بررسی گردید. همچنین، مقدار قند محلول در هر دو ژنوتیپ از طریق روش فنل- سولفوریک اسید پس از هر سطح تنش اندازه‌گیری شد. علاوه بر این، آنالیز پروموتور LTP با استفاده از ابزارهای بیوانفورماتیکی مطالعه شد. نتایج نشان داد که بیشترین سطح بیان LTP در هر دو ژنوتیپ در سطح پتانسیل اسمزی 6- بار و در 48 ساعت پس از تنش در ژنوتیپ DN-11 و 72 ساعت پس از تنش در ژنوتیپ Marvdasht بود. تفاوتی معنادار بین 48 و 72 ساعت پس از تنش در ژنوتیپ DN-11 و 72 و 3 ساعت پس از تنش در ژنوتیپ Marvdasht در سطح 1 درصد مشاهده نشد؛ اما در بین زمان‌های دیگر با اعمال تنش، در حد یک درصد تفاوتی معنادار مشاهده شد. علاوه بر این، مقدار قند محلول با افزایش سطح تنش در هر دو ژنوتیپ افزایش یافت، به طوری که مقدار آن در سطح تنش 6- بار بیشتر از شاهد بود. آنالیز پروموتور نشان داد که تعدادی از دمین‌ها و موتیف‌ها در ناحیة پروموتوری LTP وجود دارند که در پاسخ به تنش خشکی فعال می‌شوند و بیان آن را افزایش می‌دهند. بنابراین، می‌توان از ژن LTP به عنوان ژنی مقاوم به خشکی در برنامه‌های انتقال ژن و مهندسی ژنتیک استفاده کرد. | ||
| کلیدواژه‌ها [English] | ||
| Triticum aestivum, تنش خشکی, آنالیز بیان ژن, LTP, RT-qPCR | ||
| مراجع | ||
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