تعداد نشریات | 19 |
تعداد شمارهها | 379 |
تعداد مقالات | 3,110 |
تعداد مشاهده مقاله | 4,205,743 |
تعداد دریافت فایل اصل مقاله | 2,821,160 |
Application of nanoparticles (ZnO, TiO2 and CuO), a new opportunity for the stimulation of cell growth and azadirachtin production in cell suspension culture of Azadirachta indica | ||
Iranian Journal of Genetics and Plant Breeding | ||
دوره 9، شماره 1 - شماره پیاپی 17، تیر 2020، صفحه 104-113 اصل مقاله (562.87 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2021.13732.1276 | ||
نویسندگان | ||
Ghasemali Garoosi* ؛ Reza Farjaminezhad | ||
Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, P. O. Box: 34149-16818, Qazvin, Iran. | ||
تاریخ دریافت: 26 تیر 1399، تاریخ بازنگری: 17 فروردین 1400، تاریخ پذیرش: 13 بهمن 1399 | ||
چکیده | ||
Nanoparticles have unique physicochemical properties and provide great opportunities in plant science studies. In this study, we investigated the impact of ZnO, TiO2 and CuO nanoparticles (0, 20, 40, 60, and 80 mg/L) and sampling times (2, 4, and 6 days) on cell suspension growth and azadirachtin accumulation and production. Factorial experiments based on a completely randomized design with three replications were used. Results demonstrated that different nanoparticles had a different effect on the studied characters. When ZnO nanoparticles were used, the highest fresh (540.73 g/L), dry cell weight (15.93 g/L), azadirachtin accumulation (5.15 mg/g DW) and production (68.27 mg/L) were obtained at control condition, 80 and 40 mg/L ZnO nanoparticles, and control condition after 6 days, respectively. The highest amount of fresh (526.95 g/L) and dry (17.05 g/L) cell weight and azadirachtin production (82.21 mg/L) and accumulation (5.93 mg/g DW) were observed in 20 mg/L TiO2 nanoparticles, 40 mg/L of TiO2 nanoparticles after 2 days, 20 mg/L TiO2 nanoparticles in 4 days and 60 mg/L of TiO2 nanoparticles, respectively. With applying CuO nanoparticles, the highest fresh cell weight and azadirachtin accumulation were 422.59 g/L and 4.00 mg/L, achieved in control conditions respectively. Also, the highest amount of azadirachtin production was 68.27 mg/L, observed in control conditions on the 6th day of treatment. It seems that suitable cell growth, except in some cases, occurred in the absence of elicitors, but azadirachtin accumulation and production were stimulated by nanoparticles treatment. However, the results showed that the CuO nanoparticles caused a decrease in overall azadirachtin accumulation and production in the cells. | ||
کلیدواژهها | ||
Azadirachtin؛ Elicitor؛ High performance liquid chromatography؛ Nanoparticles؛ Secondary metabolites | ||
عنوان مقاله [English] | ||
کاربرد نانوذرات (ZnO، TiO2 و CuO)، یک فرصتی نو در القای رشد سلولی و تولید آزادیراختین در کشت سوسپانسیون سلولی Azadirachta indica | ||
نویسندگان [English] | ||
قاسمعلی گروسی؛ رضا فرجامی نژاد | ||
گروه بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه امام خمینی (ره)، قزوین، ایران، کد پستی: 34149-16818. | ||
چکیده [English] | ||
نانوذرات دارای ویژگیهای منحصر بفردی هستند و ظرفیتهایی فراوان در علوم گیاهی فراهم میکنند. در این جستار، تأثیر غلظتهایی از نانوذرات ZnO، TiO2 و CuO (0، 20، 40 و 80 میلیگرم در لیتر) و زمانهای نمونهبرداری (2، 4 و 6 روز) بر رشد سوسپانسیون سلولی و تولید و تجمع آزادیراختین بررسی شد. آزمایش فاکتوریل بر پایة طرح کاملا تصادفی با سه تکرار مورد استفاده قرار گرفت و نتایج نشان داد که نانوذرات مختلف دارای آثاری متفاوت روی صفات مورد مطالعه هستند. با کاربرد نانو ذره اکسید روی، بیشترین وزن تر (73/540 گرم در لیتر)، وزن خشک (93/15 گرم در لیتر) سلول، تجمع آزادیراختین ( 15/5 میلیگرم در گرم وزن خشک) و تولید آزادیراختین (27/68 میلیگرم در لیتر) به ترتیب در شاهد، 80 میلیگرم در لیتر نانوذره اکسید روی و 40 میلیگرم در لیتر نانوذره اکسید روی و شاهد بعد از 6 روز نمونهبرداری حاصل شد. با استفاده از نانوذره دیاکسید تیتانیوم، بیشترین میزان وزن تر (95/526 گرم در لیتر)، وزن خشک (05/17 گرم در لیتر) سلول، تولید آزادیراختین (21/82 میلیگرم در لیتر) و تجمع آزادیراختین (93/5 میلیگرم در گرم وزن خشک) در 20 میلیگرم در لیتر نانوذره دیاکسید تیتانیوم، 2 روز پس از افزودن 40 میلیگرم در لیتر دیاکسید تیتانیوم، 20 میلیگرم در لیتر نانوذره دی اکسید تیتانیوم در چهارمین روز پس از تیمار و 60 میلیگرم در لیتر نانوذره دی اکسید تیتانیوم مشاهده شد. با کاربرد تیمار نانوذره اکسید مس، بیشترین وزن تر سلول و تجمع آزادیراختین به ترتیب با مقدار 59/422 گرم در لیتر و 4 میلیگرم در لیتر در شاهد و بیشترین مقدار تولید آزادیراختین بهمیزان 27/68 میلیگرم در لیتر در شاهد در ششمین روز نمونهبرداری مشاهده شد. به نظر میرسد که رشد مناسب سلول به استثنای بعضی موارد در غیاب الیسیتورها رخ میدهد؛ ولی تجمع و تولید آزادیراختین با درجاتی از تیمار نانوذرات مختلف تحریک میشود. با این حال، نتایج نشان داد که در مجموع، نانوذره اکسید مس تجمع و تولید آزادیراختین را کاهش میدهد. | ||
کلیدواژهها [English] | ||
آزادیراختین, الیسیتور, کارماتوگرافی مایع با کارآیی بیشتر, نانوذرات, متابولیت های ثانویه | ||
مراجع | ||
Adhikari T., Kundu S., Biswas A. K., Tarafdar J. C., and Rao A. S. (2012). Effect of copper oxide nano particle on seed germination of selected crops. Journal of Agricultural Science and Technology, 2: 815. Bellani L. M., Muccifora S., and Giorgetti L. (2014). Response to copper bromide exposure in Vicia sativa L. seeds: analysis of genotoxicity, nucleolar activity and mineral profile. Ecotoxicology and Environmental Safety, 107: 245–250. Blum F. C., Singh J., and Merrell D. S. (2019). In vitro activity of neem (Azadirachta indica) oil extract against Helicobacter pylori. Journal of Ethnopharmacology, 232: 236–243. Castiglione M. R., Giorgetti L., Geri C., and Cremonini R. (2011). The effects of nano-TiO2 on seed germination, development and mitosis of root tip cells of Vicia narbonensis L. and Zea mays L. Journal of Nanoparticle Research, 13: 2443–2449. Chen H., Seiber J. N., and Hotze M. (2014). ACS select on nanotechnology in food and agriculture: a perspective on implications and applications Journal of Agricultural and Food Chemistry, 62: 1209–1212. Clément L., Hurel C., and Marmier N. (2013). Toxicity of TiO2 nanoparticles to cladocerans, algae, rotifers and plants–effects of size and crystalline structure. Chemosphere, 90: 1083–1090. Connolly M., Fernández M., Conde E., Torrent F., Navas J. M., and Fernández-Cruz M. L. (2016). Tissue distribution of zinc and subtle oxidative stress effects after dietary administration of ZnO nanoparticles to rainbow trout. Science of the Total Environment, 551: 334–343. Cox A., Venkatachalam P., Sahi S., and Sharma N. (2016). Silver and titanium dioxide nanoparticle toxicity in plants: a review of current research. Plant Physiology and Biochemistry, 107: 147–163. Da Costa M., and Sharma P. (2016). Effect of copper oxide nanoparticles on growth, morphology, photosynthesis, and antioxidant response in Oryza sativa. Photosynthe-tica, 54: 110–119. Deng F., Wang S., and Xin H. (2016). Toxicity of CuO nanoparticles to structure and metabolic activity of Allium cepa root tips. Bulletin of Environmental Contamination and Toxicology, 97: 702–708. Dimkpa C. O., McLean J. E., Latta D. E., Manangón E., Britt D. W., Johnson W. P., Boyanov M. I., and Anderson A. J. (2012). CuO and ZnO nanoparticles: phytotoxicity, metal speciation, and induction of oxidative stress in sand-grown wheat. Journal of Nanoparticle Research, 14: 1125. Farjaminezhad R., and Garoosi G.-a. (2019). New biological trends on cell and callus growth and azadirachtin production in Azadirachta indica. 3 Biotech, 9: 309. Ghosh M., Jana A., Sinha S., Jothiramajayam M., Nag A., Chakraborty A., Mukherjee A., and Mukherjee A. (2016). Effects of ZnO nanoparticles in plants: cytotoxicity, genotoxicity, deregulation of antioxidant defenses, and cell-cycle arrest. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 807: 25–32. Giorgetti L. (2019). Effects of nanoparticles in plants: phytotoxicity and genotoxicity assessment in: Nanomaterials in plants, algae and microorganisms. Elsevier, 65–87. Gupta S. C., Prasad S., Tyagi A. K., Kunnumakkara A. B., and Aggarwal B. B. (2017). Neem (Azadirachta indica): an indian traditional panacea with modern molecular basis. Phytomedicine, 34: 14–20. Jaberzadeh A., Moaveni P., Moghadam H. R. T., and Zahedi H. (2013). Influence of bulk and nanoparticles titanium foliar application on some agronomic traits, seed gluten and starch contents of wheat subjected to water deficit stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41: 201–207. Khan I., Saeed K., and Khan I. (2017). Nanoparticles: properties, applications and toxicities. Arabian Journal of Chemistry, In press, 18: 30–38. Kumari M., Khan S. S., Pakrashi S., Mukherjee A., and Chandrasekaran N. (2011). Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa. Journal of Hazardous Materials, 190: 613–621. Kumari M., Mukherjee A., and Chandrasekaran N. (2009). Genotoxicity of silver nanoparticles in Allium cepa. Science of the Total Environment, 407: 5243–5246. Lee S., Chung H., Kim S., and Lee I. (2013). The genotoxic effect of ZnO and CuO nanoparticles on early growth of buckwheat, Fagopyrum esculentum. Water, Air, & Soil Pollution, 224: 1668. Li M., Ahammed G. J., Li C., Bao X., Yu J., Huang C., Yin H., and Zhou J. (2016). Brassinosteroid ameliorates zinc oxide nanoparticles-induced oxidative stress by improving antioxidant potential and redox homeostasis in tomato seedling. Frontiers in Plant Science, 7: 615. Mahajan P., Dhoke S., and Khanna A. (2011). Effect of nano-ZnO particle suspension on growth of mung (Vigna radiata) and gram (Cicer arietinum) seedlings using plant agar method. Journal of Nanotechnology, 2011: 1–7. DOI: 10.1155/2011/696535. Marslin G., Sheeba C. J., and Franklin G. (2017). Nanoparticles alter secondary metabolism in plants via ROS burst. Frontiers in Plant Science, 8: 832. Mirzajani F., Askari H., Hamzelou S., Schober Y., Römpp A., Ghassempour A., and Spengler B. (2014). Proteomics study of silver nanoparticles toxicity on Oryza sativa L. Ecotoxicology and Environmental Safety, 108: 335–339. Mishra V., Mishra R. K., Dikshit A., and Pandey A. C. (2014). Chapter 8 - Interactions of nanoparticles with plants: An emerging prospective in the agriculture industry. In: Ahmad P., Rasool S. (Eds) Emerging technologies and management of crop stress tolerance. Academic Press, San Diego, 159–180. Moon Y.-S., Park E.-S., Kim T.-O., Lee H.-S., and Lee S.-E. (2014). SELDI-TOF MS-based discovery of a biomarker in Cucumis sativus seeds exposed to CuO nanoparticles. Environmental Toxicology and Pharmacology, 38: 922–931. Nair P. M. G., and Chung I. M. (2014). Impact of copper oxide nanoparticles exposure on Arabidopsis thaliana growth, root system development, root lignificaion, and molecular level changes. Environmental Science and Pollution Research, 21: 12709–12722. Nalci O. B., Nadaroglu H., Pour A. H., Gungor A. A., and Haliloglu K. (2019). Effects of ZnO, CuO and γ-Fe3O4 nanoparticles on mature embryo culture of wheat (Triticum aestivum L.). Plant Cell, Tissue and Organ Culture (PCTOC), 136: 269–277. Narendhran S., Rajiv P., and Sivaraj R. (2016). Influence of zinc oxide nanoparticles on growth of Sesamum indicum L. in zinc deficient soil. International Journal of Pharmacy and Pharmaceutical Sciences, 8: 365–371. Nazir S., Zaka M., Adil M., Abbasi B. H., and Hano C. (2018). Synthesis, characterisation and bactericidal effect of ZnO nanoparticles via chemical and bio-assisted (Silybum marianum in vitro plantlets and callus extract) methods: a comparative study. IET Nanobiotechnology, 12: 604–608. Peng C., Duan D., Xu C., Chen Y., Sun L., Zhang H., Yuan X., Zheng L., Yang Y., Yang J., Zhen X., Chen Y., and Shi J. (2015). Translocation and biotransformation of CuO nanoparticles in rice (Oryza sativa L.) plants. Environmental Pollution, 197: 99–107. Pulit-Prociak J., and Banach M. (2016). Silver nanoparticles–a material of the future…?. Open Chemistry, 14: 76–91. Rajput V., Minkina T., Suskova S., Mandzhieva S., Tsitsuashvili V., Chapligin V., and Fedorenko A. (2018a). Effects of copper nanoparticles (CuO NPs) on crop plants: a mini review. BioNanoScience, 8: 36–42. Rajput V. D., Minkina T. M., Behal A., Sushkova S. N., Mandzhieva S., Singh R., Gorovtsov A., Tsitsuashvili V. S., Purvis W. O., and Ghazaryan K. A. (2018b). Effects of zinc-oxide nanoparticles on soil, plants, animals and soil organisms: a review. Environmental Nanotechnology, Monitoring and Management, 9: 76–84. Raskar S., and Laware S. (2014). Effect of zinc oxide nanoparticles on cytology and seed germination in onion. International Journal of Current Microbiology and Applied Sciences, 3: 467–473. Ren G., Hu D., Cheng E. W., Vargas-Reus M. A., Reip P., and Allaker R. P. (2009). Characterisation of copper oxide nanoparticles for antimicrobial applications. International Journal of Antimicrobial Agents, 33: 587–590. Rico C. M., Majumdar S., Duarte-Gardea M., Peralta-Videa J. R., and Gardea-Torresdey J. L. (2011). Interaction of nanoparticles with edible plants and their possible implications in the food chain. Journal of Agricultural and Food Chemistry, 59: 3485–3498. Rizwan M., Ali S., Qayyum M. F., Ok Y. S., Adrees M., Ibrahim M., Zia-ur-Rehman M., Farid M., and Abbas F. (2017). Effect of metal and metal oxide nanoparticles on growth and physiology of globally important food crops: a critical review. Journal of Hazardous Materials, 322: 2–16. Sang L., Zhao Y., and Burda C. (2014). TiO2 nanoparticles as functional building blocks. Chemical Reviews, 114: 9283–9318. Siddiqi K. S., and Husen A. (2016). Engineered gold nanoparticles and plant adaptation potential. Nanoscale Research Letters, 11: 400. DOI: 10.1186/s11671-016-1607-2. Siddiqi K. S., and Husen A. (2017). Recent advances in plant-mediated engineered gold nanoparticles and their application in biological system. Journal of Trace Elements in Medicine and Biology, 40: 10–23. Silva S., Craveiro S. C., Oliveira H., Calado A. J., Pinto R. J. B., Silva A. M. S., and Santos C. (2017). Wheat chronic exposure to TiO2-nanoparticles: cyto- and genotoxic approach. Plant Physiology and Biochemistry, 121: 89–98. Singh A., Singh N. B., Hussain I., and Singh H. (2017). Effect of biologically synthesized copper oxide nanoparticles on metabolism and antioxidant activity to the crop plants Solanum lycopersicum and Brassica oleracea var. botrytis. Journal of Biotechnology, 262: 11–27. Sosan A., Svistunenko D., Straltsova D., Tsiurkina K., Smolich I., Lawson T., Subramaniam S., Golovko V., Anderson D., and Sokolik A. (2016). Engineered silver nanoparticles are sensed at the plasma membrane and dramatically modify the physiology of Arabidopsis thaliana plants. The Plant Journal, 85: 245–257. Srivastava S., and Srivastava A. K. (2012). Statistical medium optimization for enhanced azadirachtin production in hairy root culture of Azadirachta indica. In Vitro Cellular & Developmental Biology - Plant, 48: 73–84. Yang J., Cao W., and Rui Y. (2017). Interactions between nanoparticles and plants: phytotoxicity and defense mechanisms. Journal of Plant Interactions, 12: 158–169. Zafar H., Ali A., and Zia M. (2017). CuO nanoparticles inhibited root growth from Brassica nigra seedlings but induced root from stem and leaf explants. Applied Biochemistry and Biotechnology, 181: 365–378. Zaka M., Abbasi B. H., Rahman L.-u., Shah A., and Zia M. (2016). Synthesis and characterisation of metal nanoparticles and their effects on seed germination and seedling growth in commercially important Eruca sativa. IET Nanobiotechnology, 10: 134–140. Zhang B., Zheng L. P., Yi Li W., and Wen Wang J. (2013). Stimulation of artemisinin production in Artemisia annua hairy roots by Ag-SiO2 core-shell nanoparticles. Current Nanoscience, 9: 363–370. | ||
آمار تعداد مشاهده مقاله: 364 تعداد دریافت فایل اصل مقاله: 147 |