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Application of nanoparticles (ZnO, TiO2 and CuO), a new opportunity for the stimulation of cell growth and azadirachtin production in cell suspension culture of Azadirachta indica | ||
Iranian Journal of Genetics and Plant Breeding | ||
دوره 9، شماره 1 - شماره پیاپی 17، تیر 2020، صفحه 104-113 اصل مقاله (562.87 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2021.13732.1276 | ||
نویسندگان | ||
Ghasemali Garoosi* ؛ Reza Farjaminezhad | ||
Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, P. O. Box: 34149-16818, Qazvin, Iran. | ||
تاریخ دریافت: 26 تیر 1399، تاریخ بازنگری: 17 فروردین 1400، تاریخ پذیرش: 13 بهمن 1399 | ||
چکیده | ||
Nanoparticles have unique physicochemical properties and provide great opportunities in plant science studies. In this study, we investigated the impact of ZnO, TiO2 and CuO nanoparticles (0, 20, 40, 60, and 80 mg/L) and sampling times (2, 4, and 6 days) on cell suspension growth and azadirachtin accumulation and production. Factorial experiments based on a completely randomized design with three replications were used. Results demonstrated that different nanoparticles had a different effect on the studied characters. When ZnO nanoparticles were used, the highest fresh (540.73 g/L), dry cell weight (15.93 g/L), azadirachtin accumulation (5.15 mg/g DW) and production (68.27 mg/L) were obtained at control condition, 80 and 40 mg/L ZnO nanoparticles, and control condition after 6 days, respectively. The highest amount of fresh (526.95 g/L) and dry (17.05 g/L) cell weight and azadirachtin production (82.21 mg/L) and accumulation (5.93 mg/g DW) were observed in 20 mg/L TiO2 nanoparticles, 40 mg/L of TiO2 nanoparticles after 2 days, 20 mg/L TiO2 nanoparticles in 4 days and 60 mg/L of TiO2 nanoparticles, respectively. With applying CuO nanoparticles, the highest fresh cell weight and azadirachtin accumulation were 422.59 g/L and 4.00 mg/L, achieved in control conditions respectively. Also, the highest amount of azadirachtin production was 68.27 mg/L, observed in control conditions on the 6th day of treatment. It seems that suitable cell growth, except in some cases, occurred in the absence of elicitors, but azadirachtin accumulation and production were stimulated by nanoparticles treatment. However, the results showed that the CuO nanoparticles caused a decrease in overall azadirachtin accumulation and production in the cells. | ||
کلیدواژهها | ||
Azadirachtin؛ Elicitor؛ High performance liquid chromatography؛ Nanoparticles؛ Secondary metabolites | ||
عنوان مقاله [English] | ||
کاربرد نانوذرات (ZnO، TiO2 و CuO)، یک فرصتی نو در القای رشد سلولی و تولید آزادیراختین در کشت سوسپانسیون سلولی Azadirachta indica | ||
نویسندگان [English] | ||
قاسمعلی گروسی؛ رضا فرجامی نژاد | ||
گروه بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه امام خمینی (ره)، قزوین، ایران، کد پستی: 34149-16818. | ||
چکیده [English] | ||
نانوذرات دارای ویژگیهای منحصر بفردی هستند و ظرفیتهایی فراوان در علوم گیاهی فراهم میکنند. در این جستار، تأثیر غلظتهایی از نانوذرات ZnO، TiO2 و CuO (0، 20، 40 و 80 میلیگرم در لیتر) و زمانهای نمونهبرداری (2، 4 و 6 روز) بر رشد سوسپانسیون سلولی و تولید و تجمع آزادیراختین بررسی شد. آزمایش فاکتوریل بر پایة طرح کاملا تصادفی با سه تکرار مورد استفاده قرار گرفت و نتایج نشان داد که نانوذرات مختلف دارای آثاری متفاوت روی صفات مورد مطالعه هستند. با کاربرد نانو ذره اکسید روی، بیشترین وزن تر (73/540 گرم در لیتر)، وزن خشک (93/15 گرم در لیتر) سلول، تجمع آزادیراختین ( 15/5 میلیگرم در گرم وزن خشک) و تولید آزادیراختین (27/68 میلیگرم در لیتر) به ترتیب در شاهد، 80 میلیگرم در لیتر نانوذره اکسید روی و 40 میلیگرم در لیتر نانوذره اکسید روی و شاهد بعد از 6 روز نمونهبرداری حاصل شد. با استفاده از نانوذره دیاکسید تیتانیوم، بیشترین میزان وزن تر (95/526 گرم در لیتر)، وزن خشک (05/17 گرم در لیتر) سلول، تولید آزادیراختین (21/82 میلیگرم در لیتر) و تجمع آزادیراختین (93/5 میلیگرم در گرم وزن خشک) در 20 میلیگرم در لیتر نانوذره دیاکسید تیتانیوم، 2 روز پس از افزودن 40 میلیگرم در لیتر دیاکسید تیتانیوم، 20 میلیگرم در لیتر نانوذره دی اکسید تیتانیوم در چهارمین روز پس از تیمار و 60 میلیگرم در لیتر نانوذره دی اکسید تیتانیوم مشاهده شد. با کاربرد تیمار نانوذره اکسید مس، بیشترین وزن تر سلول و تجمع آزادیراختین به ترتیب با مقدار 59/422 گرم در لیتر و 4 میلیگرم در لیتر در شاهد و بیشترین مقدار تولید آزادیراختین بهمیزان 27/68 میلیگرم در لیتر در شاهد در ششمین روز نمونهبرداری مشاهده شد. به نظر میرسد که رشد مناسب سلول به استثنای بعضی موارد در غیاب الیسیتورها رخ میدهد؛ ولی تجمع و تولید آزادیراختین با درجاتی از تیمار نانوذرات مختلف تحریک میشود. با این حال، نتایج نشان داد که در مجموع، نانوذره اکسید مس تجمع و تولید آزادیراختین را کاهش میدهد. | ||
کلیدواژهها [English] | ||
آزادیراختین, الیسیتور, کارماتوگرافی مایع با کارآیی بیشتر, نانوذرات, متابولیت های ثانویه | ||
مراجع | ||
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