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A protocol for synthetic seed production in Rosmarinus officinalis | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 9، شماره 2 - شماره پیاپی 18، دی 2020، صفحه 126-133 اصل مقاله (484.09 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2022.16249.1304 | ||
| نویسندگان | ||
| Rasoul Azarmi1؛ Seyed Karim Tahami1، 2؛ Reza Farjaminezhad* 3؛ Younes Pourbeyrami Hir1 | ||
| 1Department of Horticulture, Faculty of Agriculture and Natural Recourses, University of Mohaghegh Ardabili, Ardabil, Iran. | ||
| 2Faculty member of Academic Center for Education, Culture & Research (ACECR), Ardabil, Iran. | ||
| 3Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), P. O. Box: 288, 34149-16818, Qazvin, Iran. | ||
| تاریخ دریافت: 11 مهر 1400، تاریخ بازنگری: 26 آذر 1400، تاریخ پذیرش: 12 بهمن 1400 | ||
| چکیده | ||
| The synthetic seeds strategy is an effective method for conservation, germplasm exchange, and distribution of Rosmarinus officinalis. R. officinalis is one of the plants of the Lamiaceae family that originates from the Mediterranean region. According to our knowledge, there is no report on the production of synthetic seeds from somatic embryos obtained from shoots of R. officinalis. In this study, a method for synthetic seed production and regeneration was investigated from shoot explants of R. officinalis. Shoots of R. officinalis were cultured on the MS medium containing various concentrations of NAA and 2,4-D. The best callus induction (41.66%) was obtained on the MS medium fortified with 1 mg/L 2,4-D. The friable embryogenic calli were transferred to a half-strength MS medium with various concentrations of BAP and 2,4-D. Somatic embryos were obtained after 45 days. The highest somatic embryogenesis was obtained on the MS medium containing 1 mg/L 2,4-D and 0.5 mg/L BAP. Somatic embryos at the torpedo stage were encapsulated in sodium alginate and synthetic seeds were obtained. The highest growth ability of synthetic seeds (80.55%) was obtained in the MS medium supplemented with 1.5 mg/L kinetin and 0.5 mg/L BAP. Moreover, the germination percentage of synthetic seeds decreased with increasing storage period and temperature. However, a higher reduction in the synthetic seed germination was recorded at 20±2 °C. The highest germination percentage of the synthetic seeds (85%) was obtained 30 days after storage at 4±1 °C. | ||
| کلیدواژه‌ها | ||
| Callus induction؛ Rosmarinus officinalis؛ Sodium alginate؛ Somatic embryo-genesis؛ Synthetic seeds | ||
| عنوان مقاله [English] | ||
| پروتکلی برای تولید بذر مصنوعی در Rosmarinus officinalis | ||
| نویسندگان [English] | ||
| رسول آذرمی1؛ سید کریم تهامی1، 2؛ رضا فرجامی نژاد3؛ یونس پوربیرامی هیر1 | ||
| 1گروه باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
| 2گروه باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.|مرکز آموزشی، فرهنگی و تحقیقاتی دانشگاه اردبیل، ایران. | ||
| 3گروه بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران، کدپستی: 16818-34149. | ||
| چکیده [English] | ||
| استراتژی بذرهای مصنوعی، روشی مؤثر برای حفاظت، تبادل ژرم‌پلاسم و توزیع Rosmarinus officinalis است. Rofficinalis، یکی از گیاهان تیره Laminaceae است که منشأ آن منطقه مدیترانه می‌باشد. بر اساس اطلاعات ما، گزارشی مبنی بر تولید بذر مصنوعی از جنین‌های سوماتیکی بدست آمده از ساقه R. officinalis وجود ندارد. در این مطالعه، یک فناوری استاندارد تولید بذر مصنوعی از ریزنمونه‌های ساقه R. officinalis و جوانه‌زنی آن مورد بررسی قرار گرفت. نوک ساقه R. officinalis روی محیط کشت MS حاوی غلظت‌های مختلف NAA و 2, 4-D کشت شدند. بهترین شرایط القای کالوس (66/41 درصد) در محیط کشت MS غنی شده با 1 میلی‌گرم در لیتر 2,4-D بدست آمد. کالوس‌های جنین‌زا به محیط کشت MS با نصف غلظت حاوی غلظت‌های مختلف BAP و 2, 4-D منتقل شدند و جنین‌های سوماتیکی، بعد از 45 روز بدست آمدند. بیشترین درصد جنین‌زایی سوماتیکی در محیط کشت MS حاوی 1 میلی‌گرم در لیتر 2.4-D و 5/0 میلی‌گرم در لیتر BAP بدست آمد. جنین‌های سوماتیکی در مرحلة اژدری‌شکل با آلژینات سدیم پوشش داده شده و بذرهای مصنوعی بدست آمد. بیشترین توانایی رشد بذرهای مصنوعی (55/80 درصد) در محیط کشت MS حاوی 5/1 میلی‌گرم در لیتر کینتین به‌همراه 5/0 میلی‌گرم در لیتر BAP بدست آمد. علاوه بر این، درصد جوانه‌زنی بذرهای مصنوعی با افزایش طول دوره ذخیره‌سازی و دما کاهش یافت. با این حال، بیشترین کاهش جوانه‌زنی بذر مصنوعی در دمای 2±20 درجه سانتیگراد مشاهده شد. بیشترین جوانه‌زنی بذر مصنوعی (85 درصد) 30 روز پس از قرارگیری در دمای 1±4 درجه سانتیگراد بدست آمد. | ||
| کلیدواژه‌ها [English] | ||
| آلژینات سدیم, القاء کالوس, بذور مصنوعی, جنین‌زایی سوماتیکی, Rosmarinus officinalis | ||
| مراجع | ||
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