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Molecular diversity and genetic structure of rainfed durum wheat genotypes using SCoT markers | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 9، شماره 2 - شماره پیاپی 18، دی 2020، صفحه 115-125 اصل مقاله (608.91 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2022.16475.1307 | ||
| نویسندگان | ||
| Zahra Morado Kheibari Moradi Kheibari1؛ Reza Azizinezhad* 1؛ Ali Mehras Mehrabi2؛ Mahmood Khosrowshahli1؛ Alireza Etminan2 | ||
| 1Department of Biotechnology and Plant Breeding, Science and Research Branch, Islamic Azad University, P. O. Box: 31749-55433, Tehran, Iran. | ||
| 2Department of Biotechnology and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran. | ||
| تاریخ دریافت: 17 آبان 1400، تاریخ بازنگری: 18 اسفند 1400، تاریخ پذیرش: 23 فروردین 1401 | ||
| چکیده | ||
| Selection- and conservation-based breeding programs require the study of genetic diversity. In this study, a collection of durum wheat consisting of 90 rainfed genotypes was subjected to the analysis of genetic diversity and population structure based on polymorphisms obtained from the Start Codon Targeted (SCoT) marker system. Out of 26 initial primers tested, 15 primers produced scorable polymorphism and were therefore, selected for further analyses. On average, 11.27 polymorphic fragments were observed for each primer per reaction. Polymorphism Information Content (PIC) ranged from 0.10 to 0.32 per locus with an average of 0.23 per primer. Resolving power (Rp) was varied from 0.98 to 5.80. The structure analysis classified the assessed population into 3 subpopulations. Besides, the Neighbor-Joining phylogenetic tree and Principal Coordinate Analysis separated genotypes into 3 and 5 distinct clusters. The Analysis of Molecular Variance (AMOVA) revealed high intra-population diversity. The gene flow index (Nm) indicated a relatively small probability of gene flow between the studied subsets. The Nei’s gene diversity (n), Shannon’s information index (I), and allele distribution statistics revealed that the individuals of subpopulation-2 had a significant capacity for genetic diversity. In conclusion, the studied SCoT primers had a high discriminating power and therefore, were efficient for evaluating genetic diversity in the durum wheat. The results of this study revealed the existence of a significant genetic diversity between the studied genotypes. Besides, the individuals of subpopulation-2 had a notable level of genetic diversity that can be used for various breeding purposes. | ||
| کلیدواژه‌ها | ||
| Gene flow؛ Inter-population differentiation؛ Neighbor-Joining algorithm؛ Principal Coordinate Analysis | ||
| عنوان مقاله [English] | ||
| تنوع مولکولی و ساختار ژنتیکی ژنوتیپ های گندم دوروم دیم با استفاده از نشانگرهای SCoT | ||
| نویسندگان [English] | ||
| زهرا مرادی خیبری1؛ رضا عزیزی نژاد1؛ علی مهراس مهرابی2؛ محمود خسروشاهلی1؛ علیرضا اطمینان2 | ||
| 1گروه بیوتکنولوژی و به‌نژادی گیاهی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران، کدپستی: 31749-55433. | ||
| 2گروه بیوتکنولوژی و به‌نژادی گیاهی، واحد کرمانشاه، دانشگاه آزاد اسلامی، کرمانشاه، ایران. | ||
| چکیده [English] | ||
| برنامه‌های اصلاحی مبتنی بر انتخاب و حفاظت از ژرم پلاسم، نیازمند مطالعه تنوع ژنتیکی هستند. در این تحقیق، تنوع ژنتیکی و ساختار جمعیت مجموعه‌ای متشکل از 90 ژنوتیپ گندم دوروم دیم بر اساس چندشکلی‌های بدست‌آمده از سیستم نشانگر هدفمند کدون آغاز (SCoT) مورد تجزیه و تحلیل قرار گرفت. از بین 26 آغازگر اولیه آزمایش شده، 15 آغازگر چندشکلی مقیاس‌پذیر ایجاد کردند و بنابراین انتخاب شدند. به‌طور متوسط در هر واکنش، 11.27 نوار چندشکل برای هر آغازگر مشاهده شد. محتوای اطلاعات چندشکلی از 0.10 تا 0.32 در هر مکان با میانگین 0.23 در هر آغازگر متغیر بود. قدرت تفکیک آغازگرها از 0.98 تا 5.80 ثبت شد. تجزیه و تحلیل ساختار، جمعیت مورد مطالعه را به 3 زیرجمعیت طبقه‌بندی کرد. علاوه بر این، تجزیه خوشه‌ای و تجزیه مختصات اصلی، ژنوتیپ‌ها را به 3 و 5 خوشه مجزا تقسیم کردند. تجزیه و تحلیل واریانس مولکولی، تنوع درون‌جمعیتی بالایی را نشان داد. شاخص جریان ژنی احتمال نسبتاً کمی از جریان ژنی بین زیر مجموعه‌های مورد مطالعه را نشان داد. تنوع ژنی Nei، شاخص اطلاعات شانون و توزیع آللها نشان داد که افراد زیرجمعیت-2 از تنوع ژنتیکی بالایی برخوردار بودند. پرایمرهای SCoT مورد مطالعه، قدرت تفکیک قابل قبولی نشان دادند؛ بنابراین برای ارزیابی تنوع ژنتیکی در گندم دوروم، کارآمد بودند. علاوه بر این، افراد زیرجمعیت-2، دارای سطح قابل توجهی از تنوّع ژنتیکی بودند که می‌توان از آن برای اهداف اصلاحی مختلف استفاده کرد. | ||
| کلیدواژه‌ها [English] | ||
| جریان ژنی, تمایز بین جمعیتی, الگوریتم ادغام همسایه ها, تحلیل مختصات اصلی | ||
| مراجع | ||
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