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Is it possible to restore the reduced coenzyme Q10 production of a varied strain of Gluconobacter? | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 10، شماره 1 - شماره پیاپی 19، تیر 2021، صفحه 31-37 اصل مقاله (711.32 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2022.16642.1310 | ||
| نویسندگان | ||
| Fouzieh Moghaddami1؛ Ramin Hosseini* 2 | ||
| 1Department of Biology, Payame Noor University, Tehran, Iran. | ||
| 2Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran. | ||
| تاریخ دریافت: 05 دی 1400، تاریخ بازنگری: 23 خرداد 1401، تاریخ پذیرش: 23 خرداد 1401 | ||
| چکیده | ||
| Different strains of a bacterial culture can undergo phenotypic variation upon continuous passages. These changes often cause a reduction or loss of bacterial metabolic potential and ultimately lead to a decrease in production yield. The aim of this study was to address this question; is it possible to restore the reduced coenzyme Q10 production of a varied strain of Gluconobacter to a higher level? The main strain studied in this study was Gluconobacter japonicus FM10, from which the FM20 strain was obtained. FM20 strain was a varied strain whose ability on coenzyme Q10 production was reduced during some continuous passages. FM30 strain was obtained from FM20 strain by restricting the available oxygen. The amount of coenzyme Q10 produced by all three strains was measured. The activities of glycerol dehydrogenase and sorbitol dehydrogenase were also measured. The results showed that coenzyme Q10 production in FM30 strain that had been exposed to restricted oxygen was several times higher (6.3 mg/L) than the varied FM20 strain (0.8 mg/L), and even the original FM10 strain (2.7 mg/L). The investigation of the enzymes activities showed that glycerol dehydrogenase and sorbitol dehydrogenase activities of FM30 strain were higher than those of the others (0.66 and 0.52 U mg-1, respectively). It can be concluded that by using some strategies, the metabolic potential of some varied strains can be restored. For strictly aerobic bacteria, Gluconobacter, the oxygen restriction may be an effective strategy for the restoration of coenzyme Q10 production ability. | ||
| کلیدواژه‌ها | ||
| Gluconobacter؛ Coenzyme Q10؛ variation؛ Dehydrogenase؛ thermotolerant | ||
| عنوان مقاله [English] | ||
| آیا امکان بازیابی توانایی تولید کوآنزیم Q10 در سویه تغییر یافته ای از Gluconobacter وجود دارد؟ | ||
| نویسندگان [English] | ||
| فوزیه مقدمی1؛ رامین حسینی2 | ||
| 1گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران. | ||
| 2گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران. | ||
| چکیده [English] | ||
| سویه های مختلف یک کشت باکتریایی می توانند بر اثر کشت‌های مکرر دچار تغییرات فنوتیپی شوند. این تغییرات اکثرا سبب کاهش یا از دست رفتن قدرت متابولیکی باکتری و نهایتا منجر به کاهش راندمان تولید می‌گردد. هدف از این مطالعه پرداختن به این سوال بود که آیا می توان در یک سویه تغییر یافته از Gluconobacter که تولید کوآنزیم Q10 در آن کاهش یافته است را به سطح بالاتر بازگرداند؟ سویه اصلی مورد بررسی در این پژوهش Gluconobacter japonicus FM10 نام داشت که سویه FM20 از آن به دست آمد. سویه FM20 سویه‌ای تغییر یافته ای بود که توانایی آن در تولید کوآنزیم Q10 طی چندین پاساژ کاهش یافته بود. سویه FM30 نیز از طریق محدود کردن اکسیژن موجود در محیط کشت از سویه FM20 بدست آمد. مقدار کوآنزیم Q10 تولید شده توسط هر سه سویه و نیز فعالیت گلیسرول دهیدروژناز و سوربیتول دهیدروژناز اندازه‌گیری شد. تولید کوآنزیم Q10 در سویه FM30 که در شرایط کمبود اکسیژن قرار گرفته بود چند برابر (3/6 میلی‌گرم در لیتر) سویه تغییر یافته یا FM20 (8/0 میلی‌گرم در لیتر) و حتی سویه اصلی یا FM10 (7/2 میلی‌گرم در لیتر) بود. همچنین، فعالیت گلیسرول دهیدروژناز و سوربیتول دهیدروژناز در سویه FM30 بیشتر از سایرین بود (به ترتیب 66/0 و 52/0 میلی گرم بر واحد). می‌توان نتیجه گرفت که با استفاده از برخی راهکارها می توان پتانسیل متابولیک سویه های تغییر یافته را بازیابی کرد. برای باکتری کاملاً هوازیGluconobacter ، محدود کردن اکسیژن ممکن است راهکار مناسبی جهت بازیابی توانایی تولید کوآنزیم Q10 باشد. | ||
| کلیدواژه‌ها [English] | ||
| Gluconobacter, کوآنزیم Q10, تغییر یا variation, دهیدروژناز, مقاوم به حرارت | ||
| مراجع | ||
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