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Phytosynthesis of magnetite (Fe3O4) nanoparticles using Nepeta bornmuelleri hairy roots | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 10، شماره 1 - شماره پیاپی 19، تیر 2021، صفحه 47-58 اصل مقاله (1.85 M) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2022.16510.1308 | ||
| نویسندگان | ||
| Shahla Atabaki1؛ Shahram Pourseyedi* 1؛ Sara Alsadat Rahpeyma1؛ Iraj Tavassolian2 | ||
| 1Department of Biotechnology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. | ||
| 2Department of Nature Engineering, Shirvan Faculty of Agriculture, University of Bojnord, Bojnord, Iran. | ||
| تاریخ دریافت: 26 آبان 1400، تاریخ بازنگری: 29 تیر 1401، تاریخ پذیرش: 30 تیر 1401 | ||
| چکیده | ||
| Magnetic iron oxide nanoparticles (Fe3O4-NPs) offer numerous applications in agriculture, pharmaceutical, and food industries. In this study, Fe3O4-NPs were effectively phytosynthesized by environmentally friendly hairy root extracts. The A4 strain of Agrobacterium rhizogenesis was used to induce the hairy roots of Nepeta bornmuelleri. Leaves in N. bornmuelleri contain important ingredients such as 1, 8 cineol that is one of the most well-known secondary metabolites. The phytosynthesis of Fe3O4-NPs was verified and described by Ultraviolet-Visible spectroscopy, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), and thermogravimetric analysis (TGA). The NPs were clustered with a size of less than 20 nm in FESEM analysis and 20-100 nm in TEM and HRTEM analysis. XRD pattern showed a peak of Fe3O4-NPs at 35.28° (220 nm). In the FTIR experiment, amines, alkanes, carbonyls, fluorides, and alcohols were detected as organic molecules involved in the synthesis of NPs. The average size of NPs in their liquid medium was about 53.39 nm, according to DLS analysis. According to TGA analysis, NPs maintained 82% of their weight at a temperature above 700 °C. According to the results of TGA study, Nepeta’s fast-growing hairy roots generated highly pure NPs. TGA analysis revealed that the weight loss of synthesized NPs in hairy roots was smaller than those identical samples produced in the extracts obtained from aerial parts of the plants. The 1, 8 cineol in the hairy root extracts measured by gas chromatography was found to be around 0.001%. | ||
| کلیدواژه‌ها | ||
| Fe3O4 nanoparticles؛ Hairy root extract؛ Nano-Phyto-synthesis؛ Nepeta bornmuelleri | ||
| عنوان مقاله [English] | ||
| بیوسنتز نانوذرات مغناطیسی آهن (Fe3O4) با استفاده از ریشه های مویین گیاه Nepeta bornmuelleri | ||
| نویسندگان [English] | ||
| شهلا اتابکی1؛ شهرام پورسیدی1؛ سارا السادات راه پیما1؛ ایرج توسلیان2 | ||
| 1گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران. | ||
| 2گروه مهندسی طبیعت، دانشکده کشاورزی شیروان، دانشگاه بجنورد، بجنورد، ایران. | ||
| چکیده [English] | ||
| نانوذرات اکسید آهن مغناطیسی (Fe3O4-NPs) کاربردهای وسیعی را در بخش‌های کشاورزی، دارویی و غذایی ارائه می‌کنند. در این کار Fe3O4-NPs با استفاده از یک رویکرد دوستدار محیط زیستی تولید در ریشه مویین، فیتوسنتز شدند. Agrobacterium rhizogenesis، سویه A4 برای القای ریشه‌های مویین گیاه Nepeta bornmuelleri، در ریزنمونه‌های برگ استفاده شد. علاوه بر این، فیتوسنتز Fe3O4-NPs با استفاده از طیف‌سنجی مرئی- فرابنفش، میکروسکوپ الکترونی روبشی نشر میدانی (FESEM)، میکروسکوپ الکترونی عبوری (TEM)، میکروسکوپ الکترونی عبوری با وضوح بالا (HRTEM)، طیف‌سنجی مادون قرمز تبدیل فوریه (FTIR)، پراش اشعه ایکس (XRD)، پراکندگی نور دینامیکی (DLS)، و تجزیه و تحلیل گرما وزنی (TGA) تأیید و توصیف شدند. نانوذرات با اندازه کمتر از 20 نانومتر در تجزیه و تحلیل FESEM خوشه بندی شدند. نانوذرات با اندازه 20-100 نانومتر در آنالیز TEM و HRTEM خوشه بندی شدند. الگوی XRD پیک در 35.28 درجه نشان می‌دهد. در آزمایش FTIR، آمین ها، آلکان ها، کربونیل ها، فلوریدها و الکل ها به عنوان مولکول های آلی درگیر در سنتز نانوذرات شناسایی شدند. بر اساس تجزیه و تحلیل DLS، اندازه متوسط نانوذرات در محیط مایع حدود 53.39 نانومتر بود. بر اساس تجزیه و تحلیل TGA، نانوذرات 82 درصد وزن خود را در دمای بالای 700 درجه سانتیگراد حفظ کردند. تجزیه و تحلیل TGA نشان داد که کاهش وزن نانوذرات سنتز شده در ریشه‌های مویین کمتر از نمونه‌های مشابه تولید شده در نواحی هوایی عصاره‌های گیاهی بود. در عصاره ریشه مویین، میانگین 1.8 سینئول با کروماتوگرافی گازی اندازه گیری شد، که حدود 0.001 درصد بود. | ||
| کلیدواژه‌ها [English] | ||
| نانو بیوسنتز, نانوذرات Fe3O4, عصاره ریشه مویین, Nepeta bornmuelleri | ||
| مراجع | ||
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