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Characterization of pollen tube growth and induced molecular changes in Berasicca napus L. by UV-B treatment | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 10، شماره 1 - شماره پیاپی 19، تیر 2021، صفحه 1-9 اصل مقاله (735.28 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2021.15126.1290 | ||
| نویسندگان | ||
| Saeid Navabpour* ؛ Dariush Ebadi Almas؛ Maryam Pakdel | ||
| Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| تاریخ دریافت: 21 اسفند 1399، تاریخ بازنگری: 08 خرداد 1401، تاریخ پذیرش: 02 آذر 1400 | ||
| چکیده | ||
| During the past few decades, the problems caused by the stratospheric ozone reduction has stimulated remarkable research on higher plant responses to UV-B radiation. Depletion of the stratospheric ozone layer is leading to an increase in ultraviolet-B (UV-B: 280–320 nm) radiation reaching the earth’s surface. This has raised interest in the possible consequences of increased UV-B levels on plant growth and development and the mechanisms underlying these responses. Although the effects of UV-B are now well characterized at the physiological level, little is known about the involved cellular and molecular mechanisms. Recent studies have shown that UV-B affects a number of important physiological processes, such as photosynthesis, through effects on gene expression. However, little research has addressed the reproductive biology of plants. The purpose of this study was to investigate the effects of UV-B radiation on reactive oxygen species (ROS) accumulation and antioxidant defense system in relation to germination and pollen tube growth of canola (Brassica napus L.). Our results illustrated that increased UV-B radiation decreased the pollen germination rate and tube length in vitro. Production of superoxide anion radical (O2•−) and hydrogen peroxide (H2O2) increased by UV-B radiation treatment, and their accumulation resulted in lipid peroxidation. The activities and gene expression of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were decreased by enhanced UV-B radiation. The increased ROS and lipid peroxidation, as well as decreased antioxidant activities may be attributed to the effects of UV-B radiation on pollen germination and tube growth. | ||
| کلیدواژه‌ها | ||
| Brassica napus L؛ Pollen germination؛ Reactive oxygen species؛ UV-B radiation | ||
| عنوان مقاله [English] | ||
| نقش گونه های اکسیژن واکنشی در جوانه زنی گرده و رشد لوله کلزا (Brassica napus L.) مهار شده توسط UV-B | ||
| نویسندگان [English] | ||
| سعید نواب پور؛ داریوش عبادی الماس؛ مریم پاکدل | ||
| گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران. | ||
| چکیده [English] | ||
| در چند دهه اخیر معضل آسیب لایه اوزون منجر به انجام مطالعات در واکنش گیاهان عالی به پرتو فرابنفش شده است. خلائ لایه اوزون درناحیه استراتوسفیک جو زمین منجر به افزایش نفوذ پرتو فرابنفش در محدوده طول موج 280 تا 320 نانومتر می‌گردد. این امر ضرورت و اهمیت مطالعه اثر پرتو فرابنفش بر گیاهان و سازوکار واکنش گیاه را افزون می نماید. اگر چه تاثیر پرتو فرابنفش بر گیاهان از نظر فیزیولوژیک بخوبی شناخته شده است، با این حال اطلاعات محدودی در سطح سلولی و مولکولی در این خصوص وجود دارد. تحقیقات اخیر از اثر پرتو فرابنفش بر فرایندهای مهم فیزولوژیک نظیر فتوسنتز و بیان برخی ژن ها گزارش می نماید. به هر حال تعداد محدودی ازاین تحقیقات در زمینه واکنش اندام زایشی به پرتو فرابنفش بوده است. هدف از این پژوهش ارزیابی اثر پرتو فرابنفش بر تولید رادیکال های فعال اکسیژن و تاثیر آن بر سیستم واکنش دفاعی در زمان جوانه زنی لوله گرده در گیاه کلزا است. نتایج نشان داد که با افزایش شدت پرتو فرابنفش سرعت جوانه زنی لوله گرده کاهش یافت. در این راستا غلظت یون سوپراکسید و پراکسید هیدروژن افزایش یافت، که منجر به بالا رفتن میزان پراکسیداسیون چربی ها شد. همچنین میزان فعالیت آنزیم های سوپراکسید دیسموتاز، کاتالاز و پراکسیداز با افزایش شدت پرتوتابی، کاهش یافت. افزایش غلظت رادیکال های فعال اکسیژن و پراکسیداسیون چربی ها همراه با کاهش فعالیت آنزیم های دفاعی می‌تواند دلیل موجهی بر روند کاهش سرعت جوانه زنی لوله گرده باشد. - - - - - - - - - - | ||
| کلیدواژه‌ها [English] | ||
| کلزا, جوانه‌زنی لوله گرده, رادیکال‌های فعال اکسیژن, پرتو‌فرا‌بنفش | ||
| مراجع | ||
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