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Analysis efficiency of Iranian Ajowan ecotypes on hairy root production mediated by different Agrobacterium rhizogenesis strains | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 10، شماره 1 - شماره پیاپی 19، تیر 2021، صفحه 117-127 اصل مقاله (913.2 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2022.17488.1325 | ||
| نویسندگان | ||
| Narges Moradi1؛ Seyed Ahmad Sadat Noori* 1؛ Ali Fadavi2؛ Seyed Mohammad Mahdi Mortazavian1؛ Ali Pakdin Parizi3 | ||
| 1Department of Agronomy and Plant Breeding Sciences, University of Tehran, Pakdasht, Tehran, Iran. | ||
| 2Department of Food Science Technology, University of Tehran, Pakdasht, Tehran, Iran. | ||
| 3Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. | ||
| تاریخ دریافت: 11 شهریور 1401، تاریخ بازنگری: 08 آذر 1401، تاریخ پذیرش: 13 آذر 1401 | ||
| چکیده | ||
| Ajowan (Trachyspermum Ammi L.) is one of the most important medicinal plants native to the Middle East and Iran. The antibiotic properties of its essential oils are related to the relatively high content of thymol. In this study, the possibility of hairy root production was investigated in a pre-experimental study considering many factors affecting transgenic efficiency, including explant type, immersion time, and the type of culture medium. Optimal conditions for the pre-experiment were set up based on the completely randomized design with seven Agrobacterium rhizogenes strains (A3, A6, A7, 4404, AATCC15834, R1000, A4) and six selected Ajowan ecotypes (Ardebil, Shiraz, Arak, Sarbishe, Qom, and Rafsanjan). The root-related morphological characteristics were measured to study the effect of main factors on the hairy root production and the bacterial strains in terms of hairy root induction. Based on the results, the highest percentage of hairy roots in Ardebil ecotype was induced by ATCC15834 strain (50%) and the lowest percentage was related to Rafsanjan ecotype induced by A6 (10%). The highest positive and significant correlation was observed in dry and wet weights (r=0.95), root frequency percentage and root length (r=0.80). The highest amount of phenolic compounds in hairy roots (240 mg/g dry weight matter) was associated with the Ardebil ecotype induced by strain ATCC15834. In this study, Shiraz and Ardebil ecotypes were identified as the best ecotypes, A4 and ATCC15834 strains as the most suitable strains, and suggested for future studies. | ||
| کلیدواژه‌ها | ||
| Agrobacterium rhizogenes؛ Correlation؛ Phenol؛ Trachyspermum Ammi L | ||
| عنوان مقاله [English] | ||
| بررسی تولید ریشه مویین اکوتیپ‌های بومی زنیان با استفاده از سویه‌های مختلف اگروباکتریوم رایزوژنز | ||
| نویسندگان [English] | ||
| نرگس مرادی1؛ سید احمد سادات نوری1؛ علی فدوی2؛ سید محمد مهدی مرتضویان1؛ علی پاکدین پاریزی3 | ||
| 1گروه زراعت و علوم اصلاح نباتات، دانشگاه تهران، پاکدشت، تهران، ایران. | ||
| 2گروه صنایع غذایی، دانشگاه تهران، پاکدشت، تهران، ایران. | ||
| 3پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران. | ||
| چکیده [English] | ||
| زنیان( (Trachyspermum Ammi L) یکی از گیاهان دارویی مهم و بومی خاورمیانه و ایران می‌باشد که خاصیت آنتی‌بیوتیکی اسانس آن مربوط به درصد نسبتا بالای تیمول است. در این مطالعه امکان القای ریشه مویین در پیش‌آزمایشی با بررسی عوامل موثر بر بازده تراریختی شامل نوع ریز‌نمونه ، مدت زمان غوطه‌وری ،نوع محیط کشت انجام گرفت و سپس شرایط بهینه برایند پیش‌آزمایش، در آزمایش فاکتوریل در قالب بلوک های کامل تصادفی، توسط هفت سویه اگروباکتریوم رایزوژنز(A3, A6, A7, 4404, AATCC15834, R1000, A4) در شش اکوتیپ منتخب زنیان(اردبیل، شیراز، اراک، سربیشه، قم و رفسنجان) با اندازه‌گیری صفات مورفولوژی مرتبط با ریشه مورد بررسی قرار گرفت. نتایج نشان داد تفاوت معنی‌داری بین اکوتیپ‌های مختلف از لحاظ میزان تحریک‌پذیری تولید ریشه مویین و همچنین بین سویه‌های باکتری از لحاظ القاء ریشه مویین وجود داشت، به طوری که بیشترین درصد ریشه مویین در اکوتیپ اردبیل القاء شده با سویه ATCC15834 (50 %) و کمترین درصد مربوط به اکوتیپ رفسنجان القا شده با سویه A6(10 %)می باشد. از بین صفات اندازه‌گیری شده بیشترین همبستگی مثبت و معنی‌دار بین صفات وزن خشک و وزن‌تر ریشه(r=0.95) ، درصد فراوانی ریشه و طول ریشه (r=0.80) مشاهده شد. بیشترین مقدار ترکیبات فنولی موجود در ریشه‌های مویین (240 میلی گرم بر گرم ماده خشک)، مربوط به اکوتیپ اردبیل تراریخت شده با سویه ATCC15834 بود. در این تحقیق اکوتیپ‌های شیراز و اردبیل به عنوان بهترین اکوتیپ‌ها و سویه‌های A4و ATCC15834به عنوان مناسب‌ترین سویه‌ها شناسایی شدند و برای مطالعات آینده پیشنهاد می‌گردد. | ||
| کلیدواژه‌ها [English] | ||
| اگروباکتریوم رایزوژنز, فنول, همبستگی, Trachyspermum Ammi L | ||
| مراجع | ||
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