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The Biological role of glycosides in alfalfa (Medicago sativa L.) as a resistance factor against alfalfa weevil (Hypera postica Gyll.) | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 6، دوره 11، شماره 1 - شماره پیاپی 21، مرداد 2022، صفحه 13-20 اصل مقاله (698.62 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2023.15387.1311 | ||
| نویسندگان | ||
| Solhaddin Jamshidi-Golan1؛ Sayyed Saeed Moosavi1؛ Hamze Mazahery-Laghab2؛ Majid Kazzazi3؛ Hojatollah Mazahery-Laghab* 1 | ||
| 1Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran. | ||
| 2Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran. | ||
| 3Department of Plant Pathology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran. | ||
| تاریخ دریافت: 20 دی 1400، تاریخ بازنگری: 05 اردیبهشت 1402، تاریخ پذیرش: 11 اردیبهشت 1402 | ||
| چکیده | ||
| After the identification of resistant, semi-susceptible, and susceptible genotypes among 42 alfalfa (Medicago sativa L.) genotypes concerning farm traits, nine genotypes were selected. Saponins obtained as a result of homogenous suspension of alfalfa tissue under maximum alfalfa weevil (Hypera postica Gyll.) attack were extracted using methanol and were purified using water-saturated butanol. The resulting solution was immobilized using the active enzyme extracted from the midgut of the pest and the thermally deactivated enzyme. In addition to the isolation of secondary glycosidic metabolites extracted from alfalfa, the enzymatic hydrolysis activity of the pest in the glycosides of genotypes were monitored using thin layer chromatography. The results of the enzymatic digestion test using TLC indicated that other than glycosides1 epigenin, other compounds were digested in the solution. The flavonoidal band of epigenin glycoside1 in all five resistant genotypes, including Tak Buteh and Ranger, was high in density and nearly equal. While maintaining the stability of the molecular structure, this compound was isolated and revealed at Rf=0.45 on a TLC plate. Therefore, midgut enzymes of alfalfa weevil were not able to digest the above compound in alfalfa. In two susceptible genotypes of Poly Cross Shiraz and Mahali Neyshaburi, the saponin band of soysaponin1 at Rf=37 had the highest density. In natural field conditions and at the time of biological stress, the lowest concentrations of saponin, soysaponin 1, and the highest concentration of flavonoids, epigenin glycoside 1, had a significant biological role in resistance to pests, and reduced damage to the cultivars under study. | ||
| کلیدواژه‌ها | ||
| Alfalfa؛ Alfalfa weevil؛ Glycosides؛ Gut enzymes؛ Saponin hydrolysis | ||
| عنوان مقاله [English] | ||
| نقش زیستی ترکیبات گلیکوزیدی یونجه (Medicago sativa L.) به عنوان عامل مقاومت در برابر حمله آفت سرخرطومی برگ (Hypera postica Gyll.) | ||
| نویسندگان [English] | ||
| صلح الدین جمشیدی گولان1؛ سید سعید موسوی1؛ حمزه مظاهری لقب2؛ مجید کزازی3؛ حجت اله مظاهری لقب1 | ||
| 1گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران. | ||
| 2دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران. | ||
| 3گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران. | ||
| چکیده [English] | ||
| پس از شناسایی ژنوتیپ‌های مقاوم، نیمه حساس و حساس از بین 42 ژنوتیپ یونجه (Medicago sativa L.) بر اساس صفات مزرعه‌ای، تعداد 9 ژنوتیپ انتخات گردیدند. ساپونین ناشی از سوسپانسیون یکنواخت بافت یونجه در شرایط اوج هجوم مزرعه‌ای آفت سرخرطومی برگ یونجه (Hypera postica Gyll.)، با استفاده از متانول استخراج و بعد به وسیله بوتانول اشباع از آب خالص سازی شد. محلول حاصل به وسیله آنزیم فعال استخراج شده از روده میانی آفت و آنزیم غیر فعال شده توسط حرارت، خوابانیده شد. تفکیک مواد متابولیکی ثانویه گلیکوسایدی استخراج شده از گیاه یونجه و همچنین، بررسی فعالیت هیدرولیزی آنزیمی آفت بر روی گلیکوساید های ژنوتیپ‌ها، به وسیله کروماتوگرافی لایه نازک مورد مطالعه قرار گرفت. نتایج آزمایش هضم آنزیمی از طریق TLC مشخص نمود که به غیر از اپیژنین گلیکوساید1، سایر ترکیب‌های موجود در محلول هضم شدند. بنابراین، باند فلاونوئیدی اپیژنین گلیکوزاید1 در تمامی 5 ژنوتیپ مقاوم از جمله ژنوتیپ‌های تک بوته و رنجر دارای غلظت بالا و تقریبا مشابه بود و با حفظ پایداری ساختمان مولکولی، در نقطه 0.45 = Rf تفکیک و تجمع یافت. در دو ژنوتیپ حساس پلی کراس شیراز و محلی نیشابوری، ، باند ساپونینی سویا ساپونین 1 در نقطه 0.37 = Rf بالاترین تراکم را داشت. در شرایط طبیعی مزرعه و در زمان تنش بیولوژیکی، کمترین غلظت ساپونین، سویاساپونین 1 و بالاترین غلظت فلاونوئید، اپیژنین گلیکوساید 1، نقش بیولوژیکی قابل توجهی در جهت مقاومت به آفت و کاهش میزان خسارت ارقام مورد مطالعه داشت. | ||
| کلیدواژه‌ها [English] | ||
| یونجه, سرخرطومی برگ, ساپونین‌ها, فلاونوئیدها, هضم آنزیمی, کروماتوگرافی لایه نازک | ||
| مراجع | ||
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