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تاثیر برخی از جدایه‌های قارچ Trichoderma harzianum بر رشد گیاه دارویی مریم گلی (Salvia officinalis L.) و القای مقاومت علیه Rhizoctonia solani | ||
| پژوهش های پیشرفته در گیاهان دارویی | ||
| دوره 2، شماره 1 - شماره پیاپی 2، خرداد 1402، صفحه 27-36 اصل مقاله (970.28 K) | ||
| نوع مقاله: مقاله علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30479/armp.2023.18666.1013 | ||
| نویسندگان | ||
| پدرام علی موسی زاده1؛ سیدجواد صانعی* 2؛ سیداسماعیل رضوی3 | ||
| 1دانشجوی کارشناسی، گروه گیاه‌پزشکی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان | ||
| 2استادیار، گروه گیاه‌پزشکی دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان | ||
| 3دانشیار، گروه گیاه‌پزشکی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان | ||
| تاریخ دریافت: 03 اردیبهشت 1402، تاریخ بازنگری: 14 خرداد 1402، تاریخ پذیرش: 21 خرداد 1402 | ||
| چکیده | ||
| در این مطالعه تأثیر شش جدایه از قارچ Trichoderma harzianum بر رشد گیاه دارویی مریم‌گلی (.Salvia officinalis L) و پاسخ‌های دفاعی گیاه علیه Rhizoctonia solani، عامل بیماری مرگ گیاهچه، بررسی شد. بذرهای مریم‌گلی پس از تیمار با جدایه‌های مختلف T. harzianum در خاک آلوده به R. solani کشت، و پس از 40 روز، وزن خشک گیاه و درصد بیماری مرگ گیاهچه بررسی شد. اندازه‌گیری مقدار کل فنول و فعالیت آنتیاکسیدانی به روش رنگ‌سنجی انجام شد. نتایج آزمایش، اثر مثبت چهار جدایه از قارچ مورد بررسی را بر افزایش وزن خشک بوته با میانگین 88/22 درصد و کاهش بیماری مرگ گیاهچه را از 50/67 به 00/41-20/14 درصد نشان داد. ارتباط بین وزن خشک کل و درصد بیماری به صورت معادله درجة 2 به دست آمد. مقدار فنول کل و فعالیت آنتیاکسیدانی در تیمار با T. harzianum افزایش داشت و تغییرات درصد بیماری و مقدار فنول کل به صورت معادلة درجه 2 محاسبه شد. جدایه‌های مختلف T. harzianum با توجه به اطلاعات مربوط به صفات مورد مطالعه در دو گروه معنی‌دار با دو زیرگروه در خوشة دوم گروه‌بندی شدند؛ به این ترتیب، پوشش بذر توسط برخی از جدایه‌های T. harzianum به‌طور قابل‌توجهی رشد می‌کند و پاسخ‌های دفاعی مریم‌گلی را افزایش می‌دهد و می‌تواند به‌عنوان رهیافتی زیست‌فناور برای بهبود رشد و مقاومت به تنش ناشی از R. solani پیشنهاد شود. | ||
| کلیدواژه‌ها | ||
| فنولِ کل؛ مرگ گیاهچه؛ مریم‌گلی؛ مهار زیستی | ||
| عنوان مقاله [English] | ||
| Effect of Trichoderma harzianum isolates on growth responses of sage (Salvia officinalis L.) and defence eliciting capacity against Rhizoctonia solani | ||
| نویسندگان [English] | ||
| Pedram Alimosazadeh1؛ Seyed Javad Sanei2؛ Seyed Esmael Razavi3 | ||
| 1B.Sc. Student, Dept. of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 2Assistant Professor, Dept. of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 3Associate Professor, Dept. of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده [English] | ||
| This experiment was designed to investigate the effect of six isolates of Trichoderma harzianum on growth and defence eliciting capacity against Rhizoctonia solani in sage (Salvia officinalis L.). The sage seeds treated with different T. harzianum isolates were planted in infested soil with R. solani and total plant dry matter and damping off severity measured 40 days after planting. The total phenolic contents and antioxidant activity were assessed by colorimetric methods. The results showed that seed treated with four T. harzianum isolates significantly increased total dry weight with average 22.88% and reduced the percent of damping off from 67.50% in infected plants to 41.00-14.20%. A quaratic model was fitted between total dry weight and disease percent. Total phenolic compounds and antioxidant activity increased with T. harzianum treatments and the changes in disease percent as a function of total phenol fitted a quadratic model. On the basis of all studied traits, different T. harzianum isolates devided in 2 groups with subgroups in second cluster. Findings indicate that the growth and defense responses of sage significantly enhanced by seed treatment with several isolates of T. harzianum and as a biotechnologic approach can be suggested to improve plant growth and R. solani resistance. | ||
| کلیدواژه‌ها [English] | ||
| Biological control, Damping off, Sage, Total phenol | ||
| مراجع | ||
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Ahmad P, Ozturk M, Sharma S, Gucel S. 2014. Effect of sodium carbonate-induced salinity-alkalinity on some key smoprotectants, protein profile, antioxidant enzymes, and lipid peroxidation in two mulberry (Morus alba L.) cultivars. Journal of Plant Interaction 9: 460-467. Al-Sohaibani SA, Mahmoud MA, Al-Othman MR, Ragab MM, Saber MM, Abd El- Aziz ARM. 2011. Influence of some biotic and abiotic inducers on root rot disease incidence of sweet basil. African Journal of Microbiology Research 5: 3628-3639. Amini Y, Mohammadi A, Zafari D. 2014. Trichoderma species associated with medicinal plants. International Journal of Advanced Biological and Biomedical Research 2: 2566-2568. Behiry S, Soliman SA, Massoud MA, Abdelbary M, Kordy AM, Abdelkhalek A, Heflish A. 2023. Trichoderma pubescens elicit induced systemic resistance in tomato challenged by Rhizoctonia solani. Journal of Fungi 9: 167. Bienkowski D, Stewart A, Falloon RE, Braithwaite M, Loguerci LL. 2010. A disease assay for Rhizoctonia solani on potato (Solanum tuberosum). New Zealand Plant Protection 63: 133-137. Brewer MT, Larkin RP. 2005. Efficacy of several potential biocontrol organisms against Rhizoctonia solani on potato. Crop Protection 24: 939-950. Cakir E, Bağdat RB, Katircioğlu YZ, Maden S. 2017. Occurrence of root rot caused by Phytophthora cryptogea on common sage (Salvia officinalis) in Turkey. Journal of Agricultural Science and Technology 7: 401-406. Cardile V, Russo A, Formisano C, Rigano D, Senatore F, Arnold NA. 2009. Essential oils of Salvia bracteata and Salvia rubifolia from Lebanon: chemical composition, antimicrobial activity and inhibitory effect on human melanoma cells. Journal of Ethnopharmacology 126: 265-72. Demirci B, Hüsnü K, Baser C, Tümen G. 2005. Composition of the essential oil of Salvia aramiensis Rech. growing in Turkey. Flavour and Fragrance Journal 17: 23-25. Drew BT, González-Gallegos JG, Xiang CL, Kriebel R, Drummond CP, Walker JB, Sytsma KJ. 2017. Salvia united: The greatest good for the greatest number. Taxon 66: 133-145. Gaderer R, Lamdan NL, Seidl-Seiboth V. 2015. Sm2, a paralog of the Trichoderma cerato-platanin elicitor Sm1, is also highly important for plant protection conferred by the fungal-root interaction of Trichoderma with maize. BMC Microbiology 15: 2-9. Harmosa R, Viterbo A, Chet I, Monte E. 2012. Plant beneficial effects of Trichoderma and its genes. Microbiology 158: 17-25. Habibi Z, Yousefi M, Aghaie HR, Salehi P, Masoudi S, Rustaiyan A. 2008. Chemical composition of essential oil of Salvia persepolitana boiss. and Salvia rhytidea Benth. from Iran. Journal of Essential Oil Research 20: 1-3. Ilham B, Noureddine C, Philippe G, Mohammed EG, Brahim E, Sophie A, Martine N, Muriel M. 2019. Induced Systemic Resistance (ISR) in Arabidopsis thaliana by Bacillus amyloliquefaciens and Trichoderma harzianum used as seed treatments. Agriculture 9: 166. Khedher MRB, Khedher SB, Chaieb I, Tounsi S, Hammami M. 2017. Chemical composition and biological activities of Salvia officinalis essential oil from Tunisia. EXCLI Journal 6: 160-173. Koochaki A, Nassiri Mahalat M, Najafi F. 2004. The agrobiodiversity of medicinal and aromatic plants in Iran. Iranian Journal of Field Crops Research 2: 208-216. (In Farsi) Kumar A, Aggarwal A, Kaushish S. 2009. Influence of arbuscular mycorrhizal fungi and Trichoderma viride on growth performance of Salvia officinalis Linn. Journal of Applied and Natural Science 1(1): 13-17. Malencic D, Popovic M, Miladinovic J. 2007. Phenolic content and antioxidant properties of soybean (Glycine max (L.) Merr.) seeds. Molecules 12: 576-581. Mastouri F, Bjorkman T, Harman GE. 2010. Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings. Phytopathology 100: 1213-1221. Mehrabi-Koushki M, Rouhani H, Mahdikhani-Moghaddam E. 2012. Differential display of abundantly expressed genes of Trichoderma harzianum during colonization of tomato-germinating seeds and roots. Current Microbiology 65: 524-533. Milat AM, Boban M, Teissedre PL, Šešelja-Perišin A, Jurić D, Skroza D, Generalić-Mekinić I, Ljubenkov I, Volarević J, Rasines-Perea Z. 2019. Effects of oxidation and browning of macerated white wine on its antioxidant and direct vasodilatory activity. Journal of Functional Foods 59: 138-147. Nadjafi F, Mahdavi Damghani M, Tabrizi L, Nejad Ebrahimi S. 2014. Effect of biofertilizers on growth, yield and essential oil content of thyme (Thymus vulgaris L.) and sage (Salvia officinalis L.). Journal of Essential Oil Bearing Plants 17 (2): 237-250. Özcan B, Birgul EM, Coleri A, Yolcu H, Caliskan M. 2009. In vitro antimicrobial and antioxidant activities of various extracts of Salvia microstegia (Boiss.) et. Bal. from Antakya, Turkey. Fresenius Environ Bulletin 18: 658-662. Pant P, Pandey S, Dall’Acqua S. 2021. The influence of environmental conditions on secondary metabolites in medicinal plants: A literature review. Chemistry and Biodiversity 18 (11): e2100345. Reuveni R, Raviv M, Krasnovsky A, Freiman L, Medina S, Bar A, Orion D. 2002. Compost induces protection against Fusarium oxysporum in sweet basil. Crop Protection 21: 583-587. Safari Motlagh MR, Abolghasemi M. 2021. The effect of Trichoderma spp. isolates on some morphological traits of canola inoculated with Sclerotinia sclerotiorum and evaluation of their efficacy in biological control of pathogen. Journal of the Saudi Society of Agricultural Sciences 21 (4): 217-231. Sanei SJ, Razavi SE. 2018. Growth responses of basil (Ocimum basilicum L.) to Trichoderma spp. and its influence in control and eliciting plant defense responses against Rhizoctonia solani. Biological Control 8 (2): 27-38. Sareena S, Poovannan K, Kumar KK. 2006. Biochemical responses in transgenic rice plants expressing a defence gene deployed against the sheath blight pathogen, Rhizoctonia solani. Current Science 91: 1529-1532. Shabana YM, Abdalla ME, Hilal AA, AbdelAziz HM. 2016. Performance of three silicon sources in suppressing Rhizoctonia solani diseases on sage (Salvia officinalis L.) and in improving yield of plant herb and essential oil. Journal of Plant protection and Pathology 7: 555-563. Taheri AH, Sanei SJ, Razavi SE. 2021. Salicylic acid is associated with improved growth and resistance of olives (Olea europaea L.) to Verticillium wilt. Journal of Plant Physiology and Breeding 11 (1): 75-85. Taheri P, Tarighi S. 2011. A survey on basal resistance and riboflavin-induced defense responses of sugar beet against Rhizoctonia solani. Journal of Plant Physiology 168: 1114-1122. Walker JB, Sytsma KJ, Treutlein J, Wink M. 2004. Salvia (Lamiaceae) is not monophyletic: implications for the systematics, radiation, and ecological specializations of Salvia and tribe Mentheae. American Journal of Botany 91: 1115-1125. Woo SL, Scala F, Ruocco M, Lorito M. 2006. The molecular biology of the interactions between Trichoderma spp., phytopathogenic fungi, and plants. Phytopathology 96: 181-185. Wu J, Ming Q, Zhai X, Wang S, Zhu B, Zhang Q, Xu Y, Shi S, Wang S, Zhang Q. 2019. Structure of a polysaccharide from Trichoderma atroviride and its promotion on tanshinones production in Salvia miltiorrhiza hairy roots. Carbohydrate Polymers 223: 115-125. Zimowska B, Bielecka M, Abramczyk B, Nicoletti R. 2020. Bioactive Products from Endophytic Fungi of Sages (Salvia spp.). Agriculture 10: 543. | ||
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