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تاثیر برخی از جدایه‌های قارچ Trichoderma harzianum بر رشد گیاه دارویی مریم گلی (Salvia officinalis L.) و القای مقاومت علیه Rhizoctonia solani | ||
| پژوهش های پیشرفته در گیاهان دارویی | ||
| دوره 2، شماره 1 - شماره پیاپی 2، خرداد 1402، صفحه 27-36 اصل مقاله (970.28 K) | ||
| نوع مقاله: مقاله علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30479/armp.2023.18666.1013 | ||
| نویسندگان | ||
| پدرام علی موسی زاده1؛ سیدجواد صانعی* 2؛ سیداسماعیل رضوی3 | ||
| 1دانشجوی کارشناسی، گروه گیاه‌پزشکی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان | ||
| 2استادیار، گروه گیاه‌پزشکی دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان | ||
| 3دانشیار، گروه گیاه‌پزشکی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان | ||
| تاریخ دریافت: 03 اردیبهشت 1402، تاریخ بازنگری: 14 خرداد 1402، تاریخ پذیرش: 21 خرداد 1402 | ||
| چکیده | ||
| در این مطالعه تأثیر شش جدایه از قارچ Trichoderma harzianum بر رشد گیاه دارویی مریم‌گلی (.Salvia officinalis L) و پاسخ‌های دفاعی گیاه علیه Rhizoctonia solani، عامل بیماری مرگ گیاهچه، بررسی شد. بذرهای مریم‌گلی پس از تیمار با جدایه‌های مختلف T. harzianum در خاک آلوده به R. solani کشت، و پس از 40 روز، وزن خشک گیاه و درصد بیماری مرگ گیاهچه بررسی شد. اندازه‌گیری مقدار کل فنول و فعالیت آنتیاکسیدانی به روش رنگ‌سنجی انجام شد. نتایج آزمایش، اثر مثبت چهار جدایه از قارچ مورد بررسی را بر افزایش وزن خشک بوته با میانگین 88/22 درصد و کاهش بیماری مرگ گیاهچه را از 50/67 به 00/41-20/14 درصد نشان داد. ارتباط بین وزن خشک کل و درصد بیماری به صورت معادله درجة 2 به دست آمد. مقدار فنول کل و فعالیت آنتیاکسیدانی در تیمار با T. harzianum افزایش داشت و تغییرات درصد بیماری و مقدار فنول کل به صورت معادلة درجه 2 محاسبه شد. جدایه‌های مختلف T. harzianum با توجه به اطلاعات مربوط به صفات مورد مطالعه در دو گروه معنی‌دار با دو زیرگروه در خوشة دوم گروه‌بندی شدند؛ به این ترتیب، پوشش بذر توسط برخی از جدایه‌های T. harzianum به‌طور قابل‌توجهی رشد می‌کند و پاسخ‌های دفاعی مریم‌گلی را افزایش می‌دهد و می‌تواند به‌عنوان رهیافتی زیست‌فناور برای بهبود رشد و مقاومت به تنش ناشی از R. solani پیشنهاد شود. | ||
| کلیدواژه‌ها | ||
| فنولِ کل؛ مرگ گیاهچه؛ مریم‌گلی؛ مهار زیستی | ||
| عنوان مقاله [English] | ||
| Effect of Trichoderma harzianum isolates on growth responses of sage (Salvia officinalis L.) and defence eliciting capacity against Rhizoctonia solani | ||
| نویسندگان [English] | ||
| Pedram Alimosazadeh1؛ Seyed Javad Sanei2؛ Seyed Esmael Razavi3 | ||
| 1B.Sc. Student, Dept. of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 2Assistant Professor, Dept. of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 3Associate Professor, Dept. of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده [English] | ||
| This experiment was designed to investigate the effect of six isolates of Trichoderma harzianum on growth and defence eliciting capacity against Rhizoctonia solani in sage (Salvia officinalis L.). The sage seeds treated with different T. harzianum isolates were planted in infested soil with R. solani and total plant dry matter and damping off severity measured 40 days after planting. The total phenolic contents and antioxidant activity were assessed by colorimetric methods. The results showed that seed treated with four T. harzianum isolates significantly increased total dry weight with average 22.88% and reduced the percent of damping off from 67.50% in infected plants to 41.00-14.20%. A quaratic model was fitted between total dry weight and disease percent. Total phenolic compounds and antioxidant activity increased with T. harzianum treatments and the changes in disease percent as a function of total phenol fitted a quadratic model. On the basis of all studied traits, different T. harzianum isolates devided in 2 groups with subgroups in second cluster. Findings indicate that the growth and defense responses of sage significantly enhanced by seed treatment with several isolates of T. harzianum and as a biotechnologic approach can be suggested to improve plant growth and R. solani resistance. | ||
| کلیدواژه‌ها [English] | ||
| Biological control, Damping off, Sage, Total phenol | ||
| مراجع | ||
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