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Stevioside and rebaudioside A production in treated hairy root culture of Stevia rebaudiana with elicitors | ||
Iranian Journal of Genetics and Plant Breeding | ||
دوره 12، شماره 1 - شماره پیاپی 23، تیر 2023، صفحه 23-35 اصل مقاله (881.93 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2023.18905.1347 | ||
نویسندگان | ||
Reza Farjaminezhad؛ Mahnaz Bagheri؛ Ghasemali Garoosi* | ||
Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), Qazvin, Iran. | ||
تاریخ دریافت: 24 خرداد 1402، تاریخ بازنگری: 02 دی 1402، تاریخ پذیرش: 02 دی 1402 | ||
چکیده | ||
Stevia rebaudiana is an important medicinal plant that is used by people with diabetes because of its steviol glycosides such as stevioside and rebaudioside A. Considering the limitation of seed production in this plant and the time-consuming nature of its propagation by stem cuttings, the use of a hairy root culture can be a suitable strategy for the production of these compounds. In this study, after the production of hairy roots by Agrobacterium rhizogenes strain ATCC15834 and their proliferation, different concentrations of methyl jasmonate (0, 50, 100, 150, and 200 µM) and salicylic acid (0, 30, 60, 90, and 120 mg/L) were applied and sampling was done at different times (24, 48, 72, and 96 h). The use of 100 μM methyl jasmonate for 48 h resulted in the accumulation of 47.31 mg/g DW, the production of 134.30 mg/L stevioside, the accumulation of 45.11 mg/g DW, and the production of 128.41 mg/L rebaudioside A and the use of 200 μM caused the accumulation of 45.56 mg/g DW and production of 120.47 mg/L rebaudioside A. While, among the different concentrations of salicylic acid, only the use of 90 mg/L for 72 h increased the accumulation of rebaudioside A (68.36 mg/g DW), and the other concentrations had a negative effect on the accumulation and production of stevioside and rebaudioside A. In conclusion, these findings showed that methyl jasmonate and salicylic acid can inhibit the growth of hairy roots and instead enhance the accumulation and production of stevioside and rebaudioside A. | ||
کلیدواژهها | ||
Candyleaf؛ Elicitor؛ High-performance liquid chromatography؛ Hairy root؛ Secondary metabolites | ||
عنوان مقاله [English] | ||
تولید استویوزید و ربادیوزیدA در ریشه های مویین تیمار شده استویا با الیسیتورها | ||
نویسندگان [English] | ||
رضا فرجامی نژاد؛ مهناز باقری؛ قاسمعلی گروسی | ||
گروه بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بینالمللی امام خمینی (ره)، قزوین، ایران. | ||
چکیده [English] | ||
استویا رباودیانا از گیاهان دارویی مهمی است که به دلیل داشتن گلیکوزیدهای استویول مانند استویوزید و ربادیوزید A مورد استفاده افراد دیابتی قرار میگیرد. با توجه به محدودیت تولید بذر در این گیاه و زمان بر بودن تکثیر آن با قلمه ساقه، استفاده از کشت ریشه مویین میتواند راهکار مناسبی برای تولید این ترکیبات دارویی باشد. در این مطالعه، پس از تولید ریشه های مویین توسط سویه ATCC15834 آگروباکتریوم ریزوژنز و تکثیر آنها، غلظتهای مختلف متیل جاسمونات (0، 50، 100، 150 و 200 میکرومولار) و اسید سالیسیلیک (0، 30، 60، 90 و120 میلیگرم در لیتر) اعمال و نمونهبرداری در زمانهای مختلف (24، 48، 72 و 96 ساعت) انجام شد. استفاده از 100 میکرومولار متیل جاسمونات به مدت 48 ساعت باعث تجمع 47/31 میلیگرم در گرم وزن خشک و تولید 134/30میلیگرم در لیتر استویوزید و تجمع 45/11 میلی گرم در گرم وزن خشک و تولید 128/41میلی گرم در لیتر ربادیوزید A و استفاده از 200 میکرومولار باعث تجمع 45/56 میلیگرم در گرم وزن خشک و تولید 120/47 میلی گرم در لیتر ربادیوزید A شد. در حالی که در بین غلظتهای مختلف اسید سالیسیلیک، تنها استفاده از 90 میلیگرم در لیتر به مدت 72 ساعت باعث افزایش تجمع ربادیوزید A (36/68 میلیگرم در گرم وزن خشک) شد و سایر غلظتها تاثیر منفی روی تجمع و تولید استویوزید و ربادیوزید A داشتند. در نتیجه، این دادهها نشان داد که متیل جاسمونات و اسید سالیسیلیک باعث مهار رشد و افزایش تجمع و تولید استویوزید و ربادیوزید A می-شوند. همچنین، سالیسیلیک اسید میتواند باعث مهار رشد و تجمع و تولید استویوزید و تولید ربادیوزید A شود. | ||
کلیدواژهها [English] | ||
مواد محرک, کروماتوگرافی مایع با کارآیی بالا, ریشه مویین, متابولیتهای ثانویه | ||
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