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Applicability of CAAT Box-derived Polymorphism Markers in Survey of Genetic Diversity in Aegilops tauschii | ||
Iranian Journal of Genetics and Plant Breeding | ||
دوره 12، شماره 2 - شماره پیاپی 24، دی 2023، صفحه 11-20 اصل مقاله (794.85 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2024.19687.1362 | ||
نویسندگان | ||
Tayebeh Shamsini-Ghiyasvand1؛ Sedigheh Fabriki-Ourang* 1؛ Jafar Ahmadi* 1؛ Ali Ashraf Ashraf Mehrabi2 | ||
1Department of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran. | ||
2Research Center of Medicinal Plants, Shahed University, Tehran, Iran. | ||
تاریخ دریافت: 19 آذر 1402، تاریخ بازنگری: 25 دی 1402، تاریخ پذیرش: 08 بهمن 1402 | ||
چکیده | ||
In this study, the molecular diversity among 95 accessions of Aegilops tauschii was investigated using a CAAT-box derived polymorphism (CBDP) marker system. Fifteen CBDP primers produced a total of 73 bands (4.87 bands per primer), of which 66 bands (91.9%) were polymorphic. The mean of polymorphic information content (PIC=0.26) and resolving power (Rp=5.62) indicated the capability of this marker system in evaluating genetic diversity in Ae. tauschii. In addition, the mean percentage of polymorphic loci, the number of observed alleles, effective alleles, the Shannon information index, and genetic diversity criterion were estimated to be 91.90%, 1.83, 1.47, 0.44, and 0.27, respectively. Analysis of molecular variance revealed that intra-population variation accounted for 96% of the total variance, while the remaining 4% was related to inter-population variation. Cluster analysis grouped the accessions into 7 main clusters. Also, according to principal coordinate analysis (PCoA), accessions were classified into six main groups, where the first two principal coordinates explained 24.19% of the total variation. The results of cluster analysis were relatively consistent with the results of PCoA. In this study, although the CBDP marker system did not completely separate Iranian accessions from other accessions however, accessions from Turkmenistan, Russia, Georgia, Turkey, Japan, and Kosovo were grouped in separate clades. Overall, the results showed that the CBDP marker system effectively identifies polymorphisms in the studied accessions. The results of this research suggest that the studied CBDP markers can be used in genetic fingerprinting and analysis of relationships between wheat and its related germplasm to evaluate various agronomic traits as well as tolerance to environmental stresses. | ||
کلیدواژهها | ||
Aegilops tauschii؛ CBDP markers؛ Genetic resources؛ Molecular markers | ||
عنوان مقاله [English] | ||
کاربرد نشانگرهای چند شکلی مشتق از جعبه CAAT در بررسی تنوع ژنتیکی Aegilops tauschii | ||
نویسندگان [English] | ||
طیبه شمسینی غیاثوند1؛ صدیقه فابریکی اورنگ1؛ جعفر احمدی1؛ علی اشرف مهرابی2 | ||
1گروه ژنتیک و به نژاد گیاهی، دانشگاه بینالمللی امام خمینی (ره)، قزوین، ایران. | ||
2مرکز تحقیقات گیاهان دارویی، دانشگاه شاهد، تهران، ایران. | ||
چکیده [English] | ||
در این مطالعه، تنوع مولکولی 95 توده Aegilops tauschii با نشانگر (CBDP (CAAT-box derived polymorphism مورد ارزیابی قرار گرفت. 15 آغازگر CBDP در مجموع 73 قطعه (87/4 قطعه برای هر آغازگر) تولید کردند که از این تعداد 66 قطعه (9/91 درصد چندشکل بودند. میانگین محتوای اطلاعات چندشکل (PIC=0.26) و قدرت تفکیک (Rp=5.62) بیانگر توانایی این سیستم نشانگری در ارزیابی تنوع ژنتیکی در Ae. tauschii بود. همچنین میانگین درصد مکانهای چندشکل، تعداد آللهای مشاهدهشده و مؤثر، شاخص اطلاعات شانون و تنوع ژنتیکی به ترتیب 90/91، 83/1، 47/1، 44/0 و 27/0 برآورد شد. تجزیه واریانس مولکولی نشان داد که %96 از تغییرات کل را تنوع درون جمعیت تشکیل میدهد، در حالی که %4 از تغییرات کل مربوط به تنوع بین جمعیت بود. تجزیه خوشهای تودهها را در هفت خوشه اصلی گروهبندی کرد. همچنین، بر اساس تجزیه مختصات اصلی (PCoA)، تودهها در شش گروه اصلی طبقهبندی شدند که دو مختصات اصلی اول %19/24 از تغییرات کل را توضیح دادند. نتایج تجزیه و تحلیل خوشهای با نتایج PCoA نسبتاً مطابق بود. در این مطالعه، اگرچه سیستم نشانگری CBDP تودههای ایرانی را به طور کامل از سایر تودهها تمایز نکرد، اما تودههای ترکمنستان، روسیه، گرجستان، ترکیه، ژاپن و کوزوو در خوشههای جداگانه گروهبندی شدند. به طور کلی، سیستم نشانگری CBDP چندشکلیها را در تودههای مطالعه شده به طور کارآمد شناسایی کرد. بنابراین نشانگرهای CBDP را میتوان در انگشت نگاری ژنتیکی ژرم پلاسم گندم برای ارزیابی صفات مختلف زراعی و نیز تحمل به تنشهای محیطی استفاده نمود. | ||
کلیدواژهها [English] | ||
منابع ژنتیکی, نشانگرهای CBDP, نشانگرهای مولکولی, Aegilops tauschii | ||
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