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Applicability of CAAT Box-derived Polymorphism Markers in Survey of Genetic Diversity in Aegilops tauschii | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 12، شماره 2 - شماره پیاپی 24، دی 2023، صفحه 11-20 اصل مقاله (794.85 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2024.19687.1362 | ||
| نویسندگان | ||
| Tayebeh Shamsini-Ghiyasvand1؛ Sedigheh Fabriki-Ourang* 1؛ Jafar Ahmadi* 1؛ Ali Ashraf Ashraf Mehrabi2 | ||
| 1Department of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran. | ||
| 2Research Center of Medicinal Plants, Shahed University, Tehran, Iran. | ||
| تاریخ دریافت: 19 آذر 1402، تاریخ بازنگری: 25 دی 1402، تاریخ پذیرش: 08 بهمن 1402 | ||
| چکیده | ||
| In this study, the molecular diversity among 95 accessions of Aegilops tauschii was investigated using a CAAT-box derived polymorphism (CBDP) marker system. Fifteen CBDP primers produced a total of 73 bands (4.87 bands per primer), of which 66 bands (91.9%) were polymorphic. The mean of polymorphic information content (PIC=0.26) and resolving power (Rp=5.62) indicated the capability of this marker system in evaluating genetic diversity in Ae. tauschii. In addition, the mean percentage of polymorphic loci, the number of observed alleles, effective alleles, the Shannon information index, and genetic diversity criterion were estimated to be 91.90%, 1.83, 1.47, 0.44, and 0.27, respectively. Analysis of molecular variance revealed that intra-population variation accounted for 96% of the total variance, while the remaining 4% was related to inter-population variation. Cluster analysis grouped the accessions into 7 main clusters. Also, according to principal coordinate analysis (PCoA), accessions were classified into six main groups, where the first two principal coordinates explained 24.19% of the total variation. The results of cluster analysis were relatively consistent with the results of PCoA. In this study, although the CBDP marker system did not completely separate Iranian accessions from other accessions however, accessions from Turkmenistan, Russia, Georgia, Turkey, Japan, and Kosovo were grouped in separate clades. Overall, the results showed that the CBDP marker system effectively identifies polymorphisms in the studied accessions. The results of this research suggest that the studied CBDP markers can be used in genetic fingerprinting and analysis of relationships between wheat and its related germplasm to evaluate various agronomic traits as well as tolerance to environmental stresses. | ||
| کلیدواژه‌ها | ||
| Aegilops tauschii؛ CBDP markers؛ Genetic resources؛ Molecular markers | ||
| عنوان مقاله [English] | ||
| کاربرد نشانگرهای چند شکلی مشتق از جعبه CAAT در بررسی تنوع ژنتیکی Aegilops tauschii | ||
| نویسندگان [English] | ||
| طیبه شمسینی-غیاثوند1؛ صدیقه فابریکی-اورنگ1؛ جعفر احمدی1؛ علی اشرف مهرابی2 | ||
| 1گروه ژنتیک و به نژاد گیاهی، دانشگاه بین المللی امام خمینی، قزوین، ایران. | ||
| 2مرکز تحقیقات گیاهان دارویی، دانشگاه شاهد، تهران، ایران. | ||
| چکیده [English] | ||
| In the current study, the genetic diversity in 95 accessions of Ae. tauschii were investigated using CAAT-box derived polymorphism (CBDP) markers. Fifteen CBDP primers produced a total of 73 bands with an average of 4.87 bands per primer, of which 66 bands were polymorphic (91.9%). The mean of polymorphic information content (PIC= 0.26) and resolving power (Rp= 5.62) indicated the ability of these markers to evaluate genetic diversity. In addition, the mean percentage of polymorphic loci, number of observed alleles, effective alleles, Shannon index, and genetic diversity were 91.90%, 1.83, 1.47, 0.44, and 0.27, respectively. In molecular variance analysis, 4% and 96% of the total variance was related to between and within populations, respectively. Genetic relationships among the studied accessions were investigated using cluster analysis. According to the cluster dendrogram obtained, 95 Ae. tauschii accessions were separated into seven main groups. By PCoA analysis, the first two principal coordinates explained 24.19% of the total variation and all accessions were placed in six main groups. The cluster analysis results were almost the same with principal coordinate analysis. Although CBDP primers could not thoroughly separate Iranian and foreign accessions into separate groups, but the accessions from the countries Turkmenistan, Russia, Georgia, Turkey, Japan, and Kosovo were grouped into separate clads. Overall, the results showed that CBDP markers could effectively identify polymorphisms in the studied accessions. Therefore, CBDP markers can be used in genetic fingerprinting and relationship analyses among wheat and its related germplasm to evaluate different agronomic traits and tolerance to stresses | ||
| کلیدواژه‌ها [English] | ||
| Aegilops tauschii, CBDP markers, Genetic resources, Molecular markers | ||
| مراجع | ||
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Ahmadi J., Pour-Aboughadareh A., Fabriki Ourang S., Khalili P., and Poczai P. (2020). Unraveling salinity stress responses in ancestral and neglected wheat species at early growth stage: A baseline for utilization in future wheat improvement programs. Physiology and Molecular Biology of Plants, 26: 537-549. Bokaei A. S., Sofalian O., Sorkhilalehloo B., Asghari A., and Pour-Aboughadareh A. (2023). Deciphering the level of genetic diversity in some Aegilops species using CAAT box-derived polymorphism (CBDP) and start codon target polymorphism (SCoT) markers. Molecular Biology Reports, 50(7): 5791-5806. DOI: 10.1007/s11033-023-08488-0. Collard B. C. Y., and Mackill D. J. (2019). Start codon targeted (SCoT) polymorphism: A simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 27: 86-93. Doyle J. J., and Doyle J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15. Eslamzadeh-Hesari M. R., Omidi M., Rashidi V., Etminan A., and Ahmadzadeh A. (2023). Investigation of molecular variability in some Aegilops species using Start Codon Targeted Polymorphism (SCoT) and CAAT-Box Derived Polymorphism (CBDP) markers. Genetika, 55(1): 19-32. Etminan A., Pour-Aboughadareh A., Mohammadi R., Noori A., and Ahmadi-Rad A. (2018). Applicability of CAAT box-derived polymorphism (CBDP) markers for analysis of genetic diversity in durum wheat. Cereal Research Communication, 46: 1-9. Etminan A., Pour-Aboughadareh A., Mohammadi R., Ahmadi-Rad A., Noori A., Mahdavian Z., and Moradi Z. (2016). Applicability of start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) markers for genetic diversity analysis in durum wheat genotypes. Biotechnology & Biotechnological Equipment, 30: 1075-1081. Etminan A., Pour-Aboughadareh A., Mehrabi A. A, Shooshtari L., Ahmadi-Rad A., and Moradkhani H. (2019). Molecular characterization of the wild relatives of wheat using CAAT-box derived polymorphism. Plant Biosyst, 153: 398-405. Ghobadi G., Etminan A., Mehrabi A. M, and Shooshtari L. (2021). Molecular diversity analysis in hexaploid wheat (Triticum aestivum L.) and two Aegilops species (Aegilops crassa and Aegilops cylindrica) using CBDP and SCoT markers. Journal of Genetic Engineering and Biotechnology, 19: 1-11. Heikrujam M., Kumar J., and Agrawal V. (2015). Genetic diversity analysis among male and female Jojoba genotypes employing gene targeted molecular markers, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP) markers. Meta Gene, 5: 90-97. Khodaee L., Azizinezhad R., Etminan A., and Khosroshahi M. (2021). Assessment of genetic diversity among Iranian Aegilops triancialis accessions using ISSR, SCoT and CBDP markers. Journal of Genetic Engineering and Biotechnology, 19: 1-5. Moradkhani H., Pour-Aboughadareh A. R., Mehrabi A. A., and Etminan A. (2012). Evaluation of genetic relationships of Triticum-Aegilops species possessing D genome in different ploidy levels using microsatellites. International Journal of Agriculture and Crop Sciences, 23: 1746-1751. Moradkhani H., Mehrabi A. A., Etminan A., and Pour-Aboughadareh A. (2015). Molecular diversity and phylogeny of Triticum –Aegilops species possessing D genome revealed by SSR and ISSR markers. Plant Breeding and Seed Science, 71: 82-95. Pandey Y., Chaturvedi T., Swaroop H., Gupta A. K., Shanker K., and Tiwari G. (2023). Phytochemical and genetic marker (SCoT and CBDP) based study of genetic diversity and population structure in natural populations of Cannabis sativa L.: A high-value sustainable biodiversity of North-Indian Himalaya. Industrial Crops and Products, 200: 116892. DOI: doi.org/10.1016/j.indcrop.2023.116892. Peakall R. O. D., and Smouse P. E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6: 288-295. Pour-Aboughadareh A., Poczai P., Etminan A., Jadidi O., Kianersi F., and Shooshtari L. (2022). An analysis of genetic variability and population structure in wheat germplasm using microsatellite and gene-based markers. Plants, 11(9): 1205. Pour-Aboughadareh A., Mahmoudi M., Moghaddam M., Ahmadi J., Mehrabi A. A., and Alavikia S. S. (2017). Agro-morphological and molecular variability in Triticum boeoticum accessions from Zagros mountains, Iran. Genetic Resources and Crop Evolution, 64: 545-556. Pour-Aboughadareh A., Omidi M., Naghavi M., Etminan A., Mehrabi A., and Poczai P. (2020). Wild relatives of wheat respond well to water deficit stress: A comparative study of antioxidant enzyme activities and their encoding gene expression. Agriculture, 10: 415. Pour-Aboughadareh A., Ahmadi J., Mehrabi A. A., Moghaddam M., and Etminan A. (2017). Evaluation of agro-morphological diversity in wild relatives of wheat collected in Iran. Journal of Agricultural Science and Technology, 19: 943-956. Pour-Aboughadareh A., Omidi M., Naghavi M. R., Etminan A., Mehrabi A. A., Poczai P., and Bayat H. (2019). Effect of water deficit stress on seedling biomass and physio-chemical characteristics in different species of wheat possessing the D genome. Agronomy, 9: 522. Pour-Aboughadareh A., Kianersi F., Poczai P., and Moradkhani H. (2021). Potential of wild relatives of wheat: Ideal genetic resources for future breeding programs. Agronomy, 11: 1656. Pour-Aboughadareh A., Ahmadi J., Mehrabi A. A., Etminan A., and Moghaddam M. (2018). Insight into the genetic variability analysis and relationships among some Aegilops and Triticum species, as genome progenitors of bread wheat using SCoT markers. Plant Biosystem, 152: 694-703. Prevost A., and Wilkinson M. J. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98: 107-112. Rajesh M. K., Sabana A. A., Rachana K. E., Rahman S., Jerard B. A., and Karun A. (2015). Genetic relationship and diversity among coconut (Cocos nucifera L.) accessions revealed through SCoT analysis. 3 Biotech, 5: 999-1006. Rholf F. (2007). Numerical taxonomy and multivariate analysis system: version 2.10. Setauket (NY): Exeter Software. Roldan-Ruiz I., Dendauw J., Van Bockstaele E., Depicker A., and De Loose M. A. F. L. P. (2000). AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding, 6: 125-134. Saeidi H., Rahiminejad M. R., Vallian S., and Heslop-Harison J. S. (2006). Biodiversity of diploid D-genome Aegilops tauschii Coss. In Iran measured using microsatellites. Genetic Resources and Crop Evolution, 53: 1477-1484. Schneider A., Molnar I., and Molnar-Lang M. (2008). Utilization of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica, 163: 1-19. Singh A. K., Rana M. K., Singh S., Kumar S., Kumar R., and Singh R. (2014). CAAT box-derived polymorphism (CBDP): A novel promoter targeted molecular marker for plants. Journal of Plant Biochemistry and Biotechnology, 23: 175-183. Varshney R. K., Chabane K., and Hendre P. S. (2007). Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Science, 173: 638-649. Yeh F. C., Yang R. C., and Boyle T. (1999). Microsoft Window-based freeware for population genetic analysis (POPGENE). Version 1.31. | ||
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