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Biostimulant impact of Trichoderma species on physiological characteristics of beans | ||
Iranian Journal of Genetics and Plant Breeding | ||
دوره 12، شماره 2 - شماره پیاپی 24، دی 2023، صفحه 37-48 اصل مقاله (882.67 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2024.19355.1356 | ||
نویسندگان | ||
Zahra Rezalou1؛ Samira Shahbazi* 2؛ Ali Asghar Aliloo1؛ Abozar Ghorbani2 | ||
1Department of Plant Production and Genetics, Faculty of Agriculture, Maraghe University, Maraghe, Iran. | ||
2Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran. | ||
تاریخ دریافت: 04 مهر 1402، تاریخ بازنگری: 18 بهمن 1402، تاریخ پذیرش: 18 بهمن 1402 | ||
چکیده | ||
This research project is dedicated to investigating innovative strategies for reducing agrochemical usage and promoting sustainable agriculture. The primary focus of this study revolves around the development and evaluation of biostimulant products derived from Trichoderma fungus. To enhance the biostimulant properties of Trichoderma, gamma irradiation was employed to induce mutations in various Trichoderma species. Subsequently, three distinct biological fertilizers were formulated using five different Trichoderma species and their respective mutants. These bio-fertilizers underwent rigorous testing to evaluate their effects on the physiological characteristics of pinto bean plants. In total, seven experimental treatments were compared to a control group. Key parameters such as soluble protein content, chlorophyll levels, carotenoid concentrations, peroxidase, and polyphenol oxidase activities were measured and analyzed. Moreover, protein profiles and enzyme subunit activities were investigated to gain deeper insights into the mechanisms underlying the observed effects. The results of this study indicate that bio-priming seeds with a combination of Trichoderma spores resulted in the most significant improvements in chlorophyll content, carotenoid levels, and peroxidase activity. Additionally, mutants of Trichoderma species exhibited greater biostimulant effects compared to their wild-type counterparts. Notably, treatments involving kaolin-based granules demonstrated higher polyphenol oxidase activity. This research emphasizes the significant impact of Trichoderma-based treatments on the physiology of pinto bean plants. The induced mutations in Trichoderma species play a crucial role in enhancing efficacy. | ||
کلیدواژهها | ||
Bio priming؛ Biostimulants؛ Trichoderma؛ Phaseolus vulgaris | ||
عنوان مقاله [English] | ||
تاثیر محرک زیستی گونه تریکودرما بر ویژگیهای فیزیولوژیکی لوبیا | ||
نویسندگان [English] | ||
زهرا رضالو1؛ سمیرا شهبازی2؛ علی اصغر علیلو1؛ ابوذر قربانی2 | ||
1گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه مراغه، ایران. | ||
2پژوهشکده کشاورزی هستهای، پژوهشگاه علوم و فنون هستهای، سازمان انرژی اتمی ایران، ایران. | ||
چکیده [English] | ||
این پروژه تحقیقاتی به مطالعه استراتژیهای نوآورانه کاهش مصرف سموم و کودهای شیمیایی در کشاورزی و توسعه کشاورزی پایدار اختصاص دارد. هدف این مطالعه، توسعه و ارزیابی فرمولاسیونهای مبتنی بر تریکودرما است. به منظور بهبود خصوصیات تریکودرما، از روش القای جهش از طریق پرتوتابی با پرتو گاما استفاده شد. سه فرمولاسیون مختلف از کود زیستی با استفاده از مخلوط پنج گونه مختلف تریکودرما (والد) و مخلوط جدایههای جهشیافته آنها تهیه شد. هفت تیمار با یک گروه کنترل مقایسه و پارامترهای کلیدی از جمله پروتئین کل، کلروفیل، کاروتنوئید، فعالیتهای پراکسیداز و پلیفنل اکسیداز اندازهگیری و آنالیز شد. علاوه بر این، پروفایلهای پروتئینی و آنزیمی گیاهان تحت تیمار با سه فرمولاسیون نیز مورد بررسی قرار گرفت تا درک عمیقتری از مکانیسمهای موثر در مشاهدات به دست آید. نتایج این مطالعه نشان میدهند که استفاده از فرمولاسیون پوششدهی بذر با مخلوط اسپورهای تریکودرمای جهشیافته، بهبود معناداری در میزان کلروفیل،کاروتنوئید و فعالیت پراکسیدازی دارد. علاوهبراین، فرمولاسیونهای مبتنی بر جدایههای جهشیافته تریکودرما نسبت به فرمولاسیونهای تهیه شده از تریکودرمای والد، اثرات محرک زیستی قویتری را نشان دادند. فرمولاسیون گرانول به طور قابل توجهی فعالیت پلیفنل اکسیدازی را افزایش داده بود. به طور کلی، این مطالعه نشان داد تیمار با تریکودرما بر فیزیولوژی لوبیای چیتی، تاثیر معنیداری داشته و کارایی روش القای جهش ژنتیکی از طریق پرتوتابی با پرتو گاما برای بهبود کارایی تریکودرما را تأیید نمود. | ||
کلیدواژهها [English] | ||
بیوپرایمینگ, محرک زیستی, تریکودرما, لوبیا | ||
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