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Assessment of genetic diversity among local accessions of melon and identification of DNA markers linked with agro-morphological characteristics | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| دوره 13، شماره 2 - شماره پیاپی 26، دی 2024، صفحه 11-20 اصل مقاله (795.78 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.30479/ijgpb.2025.20366.1371 | ||
| نویسندگان | ||
| Pedram Ajalli1؛ Nasser Mohebalipour* 1؛ Hassan Nourafcan2؛ Hamid HatamiMaleki3؛ Ali Faramarzi1 | ||
| 1Department of Agronomy and Plant Breeding, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran. | ||
| 2Department of Horticulture, Medicinal Plants and Organic Products Research Center, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran. | ||
| 3Department of Plant production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran. | ||
| تاریخ دریافت: 24 خرداد 1403، تاریخ بازنگری: 22 آذر 1403، تاریخ پذیرش: 27 دی 1403 | ||
| چکیده | ||
| In this project, 14 local melon accessions were collected from five regions of Iran and examined in a randomized complete block design with three replications in the field over two consecutive years. The combined analysis of variance showed significant differences between accessions for the majority of characters, including days to flowering, flower petal width, fruit ripening time, peduncle diameter, fruit storage at room temperature, seed width, and seed length. The interaction effect of genotype×year was significant for variables including days to flowering, leaf tail length, number of seeds per fruit, thickness of fruit flesh, fruit fresh weight, 100-seed weight, and fruit width. Among the studied characters, fruit fresh weight and fruit length were selected through stepwise regression as remarkable variables that have direct and indirect effects, respectively, on total fruit yield. Regarding principal component analysis, the first two principal components (PCs) explained 54.5% of the data variability, and the studied accessions were distinguished into two groups based on their PC1 and PC2 scores. Using 12 RAPD primers, 146 loci were amplified across the studied melon accessions. Results showed that primer OPB13, with a polymorphism information content value of 0.38, has significant power in screening local melon germplasm. Classification of the studied melon panel using the Jaccard similarity coefficient and UPGMA algorithm produced three main groups. In this study, molecular classification did not coincide with agro-morphological classification. Here, co-localized genomic loci were identified that could potentially be utilized in local melon breeding programs through marker-assisted selection. | ||
| کلیدواژه‌ها | ||
| Genetic variability؛ Marker-trait association؛ RAPD marker؛ Stepwise regression | ||
| عنوان مقاله [English] | ||
| بررسی تنوع ژنتیکی در میان توده‌های بومی ملون و تعیین نشانگرهای DNA مرتبط با خصوصیات آگرومورفولوژیک | ||
| نویسندگان [English] | ||
| پدرام اجلی1؛ ناصر محبعلی پور1؛ حسن نورافکن2؛ حمید حاتمی ملکی3؛ علی فرامرزی1 | ||
| 1گروه زراعت و اصلاح نباتات، واحد میانه، دانشگاه آزاد اسلامی، میانه، ایران. | ||
| 2گروه علوم باغبانی، مرکز تحقیقات گیاهان دارویی و محصولات ارگانیک، واحد میانه، دانشگاه آزاد اسلامی، میانه، ایران. | ||
| 3گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران. | ||
| چکیده [English] | ||
| در این پروژه 14 توده خربزه محلی از 5 منطقه ایران جمع آوری و در قالب طرح RCBD با سه تکرار در حالت مزرعه طی دو سال متوالی مورد بررسی قرار گرفت. تجزیه و تحلیل ترکیبی واریانس تفاوت معنی‌داری را بین اکسشن‌ها برای اکثر صفات از جمله DF، FPW، FRT، PD، FSRT، SW و SL نشان داد. اثر متقابل ژنوتیپ × سال برای متغیرهای 50% DF، LTL، NSPF، TFF، FFW، 100 SW و FW معنی‌دار تشخیص داده شد. از بین صفات مورد مطالعه، FFW و FL با استفاده از رگرسیون گام به گام به عنوان متغیر قابل توجه انتخاب شدند که به ترتیب تأثیر مستقیم و غیرمستقیم بر عملکرد کل میوه خربزه دارند. با توجه به تجزیه و تحلیل PCA، دو PC اول 54.5 درصد از تنوع داده ها را توضیح دادند و پیوست های مورد مطالعه بر اساس امتیاز PC1 و PC2 در دو گروه تفکیک شدند. با استفاده از 12 پرایمر RAPD، 146 جایگاه از طریق توده های خربزه مورد مطالعه تکثیر شد. نتایج نشان داد پرایمر OPB13 با مقدار PIC 0.38 قدرت قابل توجهی در غربالگری ژرم پلاسم محلی خربزه دارد. طبقه بندی پانل خربزه مورد مطالعه با استفاده از ضریب شباهت جاکارد و الگوریتم UPGMA سه گروه اصلی را تولید کرده است. در این مطالعه، طبقه بندی مولکولی با طبقه بندی زراعی-مورفولوژیکی منطبق نبود. تجزیه و تحلیل ارتباط نشانگر-ویژگی جایگاه های ژنومی هم موضعی را نشان داد که به طور بالقوه می توانند در برنامه های اصلاح نژاد خربزه از طریق انتخاب به کمک نشانگر مورد استفاده قرار گیرند. | ||
| کلیدواژه‌ها [English] | ||
| تنوع ژنتیکی, رگرسیون گام به گام, نشانگر RAPD, ارتباط صفت-نشانگر | ||
| مراجع | ||
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