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Expression of related proteins and aquaporin genes in grape (Vitis vinifera L.) under salinity sress | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 2، دوره 3، شماره 2 - شماره پیاپی 6، دی 2014، صفحه 8-18 اصل مقاله (821.83 K) | ||
نویسنده | ||
Nayer Mohammadkhani* | ||
Shahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, Iran, P.O. Box 196, Postal Code: 5978159111. | ||
تاریخ دریافت: 22 فروردین 1395، تاریخ پذیرش: 22 فروردین 1395 | ||
چکیده | ||
Due to worldwide increasing of salinity, the identification of genes conferring tolerance to plants is important. The aim of this study was to investigate salinity effects on the expression of three genes-related to proteins and aquaporin in grape (Vitis vinifera L.). Based on screening study on 18 grape genotypes, H6 and Gharashani that showed lower decrease in water potential, leaf area, leaf growth rate and relative water content under salinity, were selected as tolerant genotypes compared to sensitive ones (Shirazi and GhezelUzum). Plants were treated with 50 mM NaCl as a critical concentration for 0, 24 hours and 14 days. High expression of VvFS41 gene in the sensitive genotypes under salinity showed that these genotypes used all of their capacity to survive under stress condition. The expression of VvPHP1 showed no regular status. Expression of VvPIP2.2 decreased in roots of all genotypes under salinity and the determination of VvPIP2.2 role seems be difficult in these genotypes. Based on the results, sensitive genotypes showed higher changes in proteins and aquaporin genes under salinity, but tolerant genotypes was more stable. Our findings showed a significant difference between tolerant and sensitive genotypes and highlighted a strong relationship between the accumulation of specific transcripts and stress tolerance. | ||
کلیدواژهها | ||
Salinity؛ Grape؛ Stress proteins؛ Transcripts؛ Aqua-porin genes | ||
عنوان مقاله [English] | ||
بیان پروتئینهای مربوطه و ژنهای آکواپورین در انگور (Vitis vinifera L.) تحت تنش شوری | ||
نویسندگان [English] | ||
نیر محمدخانی | ||
مرکز آموزش عالی شهید باکری میاندوآب، دانشگاه ارومیه. | ||
چکیده [English] | ||
به دلیل افزایش شوری خاک در سرتاسر جهان شناسایی ژنهای دخیل در تحمل گیاهان مهم است. هدف مطالعه بررسی اثرات شوری بر بیان سه ژن -مربوط به پروتئینها و آکواپورین- در انگور (Vitis vinifera L.) بود. بر اساس آزمایشهای غربالگری روی 18 ژنوتیپ H6 و قرهشانی که کاهش کمتری در پتانسیل آب، سطح برگ، سرعت رشد برگ و محتوای آب نسبی نشان دادند، به عنوان ژنوتیپهای متحمل در مقایسه با حساس (شیرازی و قزل اوزوم ) انتخاب شدند. گیاهان با 50 میلی مول NaCl – بهعنوان غلظت مناسب - به مدت 0، 24 ساعت و 14 روز تیمار شدند. بیان بالای ژن VvFS41 در ژنوتیپهای حساس تحت شوری نشان داد که این ژنوتیپها حداکثر ظرفیت خود را برای بقا در شرایط شور به کار میبرند. بیان VvPHP1 هیچ نظم خاصی نداشت. بیان VvPIP2.2در ریشه همه ژنوتیپها تحت شوری کاهش یافت و تعیین نقش VvPIP2.2 در ژنوتیپها مشکل بود. براساس نتایج ژنوتیپهای حساس تحت شوری تغییرات بیشتری در ژنهای پروتئینها و آکواپورین نشان دادند، اما ژنوتیپهای متحمل باثبات تر بودند. یافتههای ما تفاوت معنیداری بین ژنوتیپهای حساس و متحمل نشان داد و ارتباط قوی بین انباشتگی ژنها و درجه تحمل به شوری را برجسته کرد. | ||
کلیدواژهها [English] | ||
شوری, انگور, پروتئینهای تنشی, رونوشتها, ژنهای آکواپورین | ||
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