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Assessment of genetic diversity in Pythium aphanidermatum isolates using ISSR and rep-CR methods | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 5، دوره 3، شماره 2 - شماره پیاپی 6، دی 2014، صفحه 41-52 اصل مقاله (758.5 K) | ||
| نویسندگان | ||
| Massoume Amini1؛ Naser Safaie2؛ Haidar Saify Nabiabad* 1 | ||
| 1Department of Biotechnology, College of Agriculture, Bu-Ali Sina University, Hamadan, Iran, Postal code: 651783- 8695. | ||
| 2Department of plant pathology, College of Agriculture, Tarbiat Modares University, Tehran, Iran, Postal code: 111-14115. | ||
| تاریخ دریافت: 22 فروردین 1395، تاریخ پذیرش: 22 فروردین 1395 | ||
| چکیده | ||
| Sixty isolates of Pythium aphanidermatum as the causal agent of sugar beet root rot were selected on the basis of their geographical origins, morphological and genetic diversity studies. Pathogenicity test using the Kruskal-Wallis analysis showed significant differences between the pathogenic potential of isolates. Based on the growth rate, isolates were categorized in two groups with low and high growth rate. The potential of ISSR and rep-PCR for fingerprinting purposes was evaluated using eight ISSR and three rep-PCR primers. A strong linear relationship was observed between resolving power of a primer, PIC, and MI. PIC value for the rep-PCR primers (0.41) was higher than the ISSR primers (0.39). Based on UPGMA method and Jaccard's coefficient, ISSR separated Khuzestan and Fars provinces isolates from other isolates at 63% similarity level, while rep-PCR separated these isolates at 61% similarity level. The PCA analysis showed that despite the similarities of both markers, rep-PCR represent a higher resolution than the ISSR. So the rep-PCR marker leads to further differentiating of the isolates. There was a relationship between genetic divergence and geographical origins of isolates. rep-PCR can replace ISSR in diversity studies because of their comparable accuracy in the interpretation of genetic diversity of isolates. | ||
| کلیدواژه‌ها | ||
| Comparative analysis؛ Pythium aphanidermatum؛ ISSR؛ Repetitive element-PCR | ||
| عنوان مقاله [English] | ||
| بررسی تنوع ژنتیکی جدایههای Pythium aphanidermatum با استفاده از روش‌های ISSR و rep-PCR | ||
| نویسندگان [English] | ||
| معصومه امینی1؛ ناصر صفایی2؛ حیدر سیفی نبی آباد1 | ||
| 1گروه بیوتکنولوژی، دانشکده کشاورزی دانشگاه بوعلی سینا همدان. | ||
| 2گروه بیماری شناسی، دانشکده کشاورزی دانشگاه تربیت مدرس. | ||
| چکیده [English] | ||
| 60 جدایه Pythium aphanidermatum، عامل پوسیدگی ریشه چغندرقند، بر اساس منشاء جغرافیایی برای مطالعات تنوع ژنتیکی و مورفولوژیکی انتخاب شدند. آزمون بیماریزایی با استفاده از آنالیز کروسکال والیس اختلاف معنیداری بین جدایهها از لحاظ قدرت بیماریزایی نشان داد. جدایهها براساس سرعت رشد به دو گروه با میزان رشد بالا و میزان رشد پایین گروهبندی شدند. به منظور بررسی پتانسیل انگشت نگاری ISSR و rep-PCR از هشت آغازگر ISSR و سه آغازگر rep-PCR استفاده شد. همبستگی خطی بسیار بالایی بین قدرت تفکیک، PIC و MI هر آغازگر مشاهده شد. شاخص PIC برای آغازگرهای rep-PCR (41/0) بالاتر از آغازگرهای ISSR (39/0) بود. براساس روش UPGMA و ضریب جاکارد، ISSR جدایههای استانهای خوزستان و فارس را در سطح تشابه 63% از بقیه استانها جدا کرد، درحالیکه rep-PCR این جدایهها را در سطح تشابه %61 از بقیه استانها جدا کرد. آنالیز PCA نشان داد که باوجود تشابههای هر دو نشانگر، rep-PCR قدرت تفکیک بالاتری نسبت به ISSR نشان داد. بنابراین نشانگر rep-PCR منجر به تفکیک بیشتر جدایهها شد. بین تنوع ژنتیکی و منشاء جغرافیایی جدایهها ارتباط وجود دارد. در مطالعات تنوع ژنتیکی، Rep-PCR به دلیل داشتن دقت مقایسهای در تفسیر تنوع ژنتیکی جدایهها، میتواند جایگزین ISSR شود. | ||
| کلیدواژه‌ها [English] | ||
| آنالیز مقایسهای, Pythium aphanidermatum, ISSR, Rep-PCR | ||
| مراجع | ||
|
Afzali, H., and Ershad, D. (2006). The causal agents of root rot and damping-off sugar beet in Khorasan province and relation of plant age and Pythium spp. Pathogencity (17th Iranian Plant Protection Congress), Iran, 2-5 Sept.
Ahkami, A.M., Naghavi, M.R., Mardi, M.A., Hosseinzadeh, M., Pirsayedi, P., Petki, M., Kazemi, A., Hashempour, I., and Omidbakhsh, M.A. (2007). Study of genetic relatively in durum wheat (Triticum durum) using AFLP markers. Iranian Journal of Agricultural Sciences, 1: 25-35.
Al-sadi, A.M. (2007). Population diversity, sources and evaluation of metalaxyl-based management of Pythium damping-off of cucumber in oman. Plant pathology, 57: 45-56.
Al-sadi, A.M., Drenth, A., Deadman, M.L., and Aitken, A.B. (2008). Genetic diversity, aggressiveness and metalaxyl sensitivity of Pythium aphanidermatum populations infecting cucumber in oman. Plant pathology, 57: 45-56.
Babaie-Ahary, A., Abrinnia, M., and Majidi Heravan, I. (2004). Identification and pathogenicity of Pythium species causing damping-off in sugarbeet in northwest Iran. Australasian Plant Pathology, 33: 343-347.
Barth, S., Melchinger, A.E., and Luebberestedt, T. (2002). Genetic diversity in Arabidopsis thaliana L. Heynh. Investigated by cleaved amplified polymorphic sequence (CAPS) and intersimple sequence repeat (ISSR) markers. Molecular Ecology, 11: 495- 505.
Botstein, D., White, R.L., Skolnick, M., and Davis, R.W. (1980). Construction of genetic linkage map in man using restriction length polymorphisms. American Journal of Human Genetics, 32: 314-331.
Buttner, G., Pfahler, B., and Marlande, B. (2004). Greenhouse and field techniques for testing sugar beet for resistance to Rhizoctonia root and crown rot. Plant Breeding, 123: 158-166.
De Bruijn, F. (1992). Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria. Applied and Environmental Microbiology, 58: 2180–2187.
Esselman, E.J., Jianqiang, L., Crawford, D.J., Winduss, J.L., and Wolfe, A.D. (1999). Clonal diversity in the rare Calamagrostis porteri ssp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Molecular Ecology, 8: 443-451.
Garzon, C.D., Geiser, D.M., and Moorman, G.W. (2005). Diagnosis and Population Analysis of Pythium Species Using AFLP Fingerprinting. Pennsylvania State University, University Park, 16802, U.S.A.
Garzon, C.D., Yanez, J.M., and Moorman, G.W. (2007). Pythium cryptoirregular, a new species within the P. irregulare complex. Mycologia, 99(2): 291-301.
Gilson, E., Clement, J.M., Brutlag, D., and Hofnung, M. (1984). A family of dispersed repetitive extragenic palindromic DNA sequences in E.coli. European Molecular Biology Organization, 3:1417–1421.
Groth, D.E., and Bond, J.A. (2007). Effects of cultivars and fungicides on rice sheath blight, yield, and quality. Plant Disease, 91:1647-1650.
Harvey, P.R., Butterworth, P.J., Hawke, B.G., and Pankhurst, C.E. (2000). Genetic variation among populations of Pythium irregular in southern Australia. Plant Pathology, 49: 619-627.
Jaccard, P. (1908). Nouvelles researches sue la distribution florale. Société vaudoise des sciences naturelles, 44: 223–270. Kang, H.W., Park, D.S., Go, S.J., and Eun, M.Y. (2002). Fingerprinting of diverse genomes using PCR with universal rice primers generated from repetitive sequences of Korean weedy rice. Molecules and Cells, 13: 281–287.
Khodayari, M., Safaie, N., and Shamsbakhsh, M. (2009). Genetic Diversity of Iranian AG1-IA Isolates of Rhizoctonia solani, the Cause of Rice Sheath Blight, Using Morphological and Molecular Markers. Journal of Phytopathol, 157: 708–714.
Lee, S., and Moorman, G.W. (2008). Identification and characterization of simple sequence repeat markers for Pythium aphanidermatum, P. cryptoirregulare and P. irregulare and the potential use in Pythium population genetics. Current Genetics, 53(2): 81-93.
Levesque, C.A., and de Cock, A.W.A.M. (2004). Molecular phylogeny and taxonomy of the genus Pythium. Mycology Research, 108: 1363-1383.
Lynch, M., and Walsh, J.B. (1998). Genetics and analysis of Quantitative trates. Sinauer Assocs., Inc., Sunderland MA.
Maguire, T.L., Peakall, R., and Saenger, P. (2002). Comparative analysis of genetic diversity in the mangrove species Avicennia marina (Forsk.) Vierh. (Avicenniaceae) detected by AFLPs and SSRs. Theoretical and applied Genetics, 104: 338-398.
Mantel, N. (1967). The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209-220.
Martin, F.N. (2000). phylogenetic relationships among some Pythium species inferred from sequence analysis of the mitochondrially encoded cytochrome oxidase II gene. Mycologia, 92: 711-727.
Martin, B., Humbert, O., Camara, M., Guenzi, E., Walker, J., Mitchell, T., Andrew, P., Prudhomme, M., Alloing, G., Hakenbeck, R., Morrison, D.A., Boulnois, G.J., and Claverys, J.P. (1992). A highly conserved repeated DNA element located in the chromosome of Streptococcus pneumoniae. Nucleic Acids Research, 20: 3479–3483.
Martinez, F.N., and English, J.T. (1997). Population genetics of soilborne fungal plant pathogens (Introduction). Phytopathology, 87: 446-447.
Peakall, R., and Smouse, P.E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6: 288-295.
Powel, W., Morgante, M., Andre, C., Vogel, J., Tingey, S., and Rafalski, A. (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsattlite) markers for germplasm analysis. Molecular breeding, 2: 225-238.
Prevost, A., and Wilkinson, M.J. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and applied Genetics, 98: 107–112.
Rodriguez-Barradas, M.C., Hamill, R.J., Houston, E.D., Georghiou, P.R., Clarridge, J.E., Rengery, R.L., and Koehler, J.E. (1995). Genomic fingerprinting of Bartonella species by repetitive element PCR for distinguishing species and isolates. Journal of Clinical Microbiology, 33: 1089–1093.
Rohlf, F.J. 1993. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 1.80. Setauket, NY, Exeter Biological Software.
Safaie, N., Alizade, A., Saidi, A., Rahimian, H., and Adam, G. (2005). Molecular characterization and genetic diversity among Iranian populations of Fusarium graminearum, the causal agent of wheat head blight. Iranian Jour-
nal Plant Pathology, 41: 171–189.
Sharples, G. J., and Lloyd, R.G. (1990). A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes. Nucleic Acids Research, 18: 6503– 6508.
Sheikholeslami, M., Younesi, H., and Ershad, J. (2002). Identification of fungi involving sugar beet rot and their distribution in Kermanshah Provinc.15th Iranian Plant Protection Congress, 133p.
Stern, M.J., Ames, G.L., Smith, N.H., Robinson, E.C., and Higgins, C.F. (1984). Repetitive extragenic palindromic sequences: a major component of the bacterial genome. Cell, 37: 1015–1026.
Van Der Plaats- Niterink, A.J. (1982). Monograph of the genus Pythium. Studies in Mycology. Studies in Mycology, 21: 1-242.
Vasserur, V., Rey, P., Bellanger, E., Brygoo, Y., and Tirilly, Y. (2005). Molecular characterization of Pythium group F isolates by ribosomal- and intermicrosatellite-DNA regions analysis. European Journal of Plant Pathology, 112: 301-310.
Versalovic, J., Schneider, M., De Bruijn, F.J., and Lupski, J.R. (1994). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods in Molecular and Cellular Biology, 5: 25–40.
Von Bretzel, P., Stanghellini, M.E., and Kroland, W.C. (1988). Epidemilogy of Pythium root rot of suger beets. Plant Disease, 72: 707-709.
Wang, P.H., Wang, Y.T., and White, J.G. (2003). Species-specific PCR primers for Pythium developed from ribosomal ITS1 region. Letters in Applied Microbiology, 37: 127–132.
Zamani Noor, N., Minassian, V., Banihashemi, Z., and Mostofizadeh, R. (2004). Identification and pathogencity of Pythium species on sugar beet in Khuzestan province. General Plant Pathology, 40:179-200.
Zietkiewicz, E., Fafalski, A., and Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20: 176-183. | ||
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