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Variability in antioxidant activity and phenolic profile in different parts of sunflower (Helianthus annuus L.) genotypes | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 6، دوره 3، شماره 2 - شماره پیاپی 6، دی 2014، صفحه 53-60 اصل مقاله (420.81 K) | ||
| نویسندگان | ||
| Asaad Aghaei1؛ Reza Darvishzadeh* 2، 3؛ Ali Asghar Hatamnia4؛ Farhad Ghavami5 | ||
| 1Department of Biology, Faculty of Science, Urmia University, Urmia, Iran. | ||
| 2Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran. | ||
| 3Institute of Biotechnology, Urmia University, Urmia, Iran. | ||
| 4Department of Biology, Faculty of Science, Ilam University, Ilam, Iran. | ||
| 5Chief Science Officer (CSO) Eurofins BioDiagnostics Inc, River Falls, Wisconsin, USA. | ||
| تاریخ دریافت: 22 فروردین 1395، تاریخ پذیرش: 22 فروردین 1395 | ||
| چکیده | ||
| Sunflower (Helianthus annuus L.) is largely consumed in Iran as an oleaginous and confectionery product. In this study, the total phenolic and flavonoid contents, antioxidant activity and phenolic composition in different parts of the seeds from six sunflower genotypes were investigated. The antioxidant activity of extract was evaluated by different assays. The total phenolic and flavonoid contents in kernel were 6.8 and 4.1 times higher than those in shell. Antioxidant activity of kernel extract was significantly higher than shell. It can be attributed to higher total phenolic and flavonoid content. In kernel extracts, a positive correlation coefficient was observed between total phenolic content and FRAP (r = 0.636) and Nitric oxide radical inhibition (r= 0. 721) assays. The amount of identified phenolic compounds varied in different extracts and ranged from 0.29 µg/g (Vanillic acid, shell of S5 genotype) to 433.6 µg/g (Syringic acid, kernel of S2 genotype). Therefore, the result indicated that kernel sunflower seeds can be used as potent natural antioxidants in diet. | ||
| کلیدواژه‌ها | ||
| Antioxidant activity؛ Kernel؛ Phenolic composition؛ Shell؛ Sunflower | ||
| عنوان مقاله [English] | ||
| تنوع در فعالیت ضداکسایشی و ترکیب فنلی قسمتهای مختلف ژنوتیپ‌های آفتابگردان (.Helianthus annuus L) | ||
| نویسندگان [English] | ||
| اسعد آقایی1؛ رضا درویش زاده2، 3؛ علی اصغر حاتم نیا4؛ فرهاد قوامی5 | ||
| 1دانش آموخته کارشناسی ارشد فیزیولوژی گیاهی، گروه زیست شناسی دانشکده علوم دانشگاه ارومیه. | ||
| 2استاد گروه اصلاح و بیوتکنولوژی گیاهی دانشکده کشاورزی دانشگاه ارومیه. | گروه بیوتکنولوژی کشاورزی پژوهشکده زیست فناوری دانشگاه ارومیه. | ||
| 3استاد گروه اصلاح و بیوتکنولوژی گیاهی دانشکده کشاورزی دانشگاه ارومیه. | گروه بیوتکنولوژی کشاورزی پژوهشکده زیست فناوری دانشگاه ارومیه. | ||
| 4استادیار گروه زیست شناسی دانشکده علوم دانشگاه ایلام. | ||
| 5معاونت علوم و فناوری شرکت یوروفینز بیو دایاگنوستیک، ریور فالز، ویسکانسین، آمریکا. | ||
| چکیده [English] | ||
| آفتابگردان (.Helianthus annus L) در ایران اکثرا به صورت یک محصول روغنی و آجیلی به مصرف میرسد. در این مطالعه محتوی فنل و فلاوونوئید کل، فعالیت ضداکسایشی و ترکیب فنلی در قسمتهای مختلف دانه شش ژنوتیپ آفتابگردان مورد ارزیابی قرار گرفت. فعالیت ضداکسایشی عصارهها با استفاده از سنجشهای متفاوتی ارزیابی گردید. محتوی فنل و فلاوونوئید کل در مغز دانه به ترتیب 8/6 و 1/4 برابر میزان آنها در پوسته بود. فعالیت ضداکسایشی عصاره مغز به طور معنیداری بیشتر از عصاره پوسته بوده که میتوان آن را به میزان بالای محتوی فنل و فلاوونوئید نسبت داد. در عصارههای مغز، ضریب همبستگی مثبت بین محتوی فنل کل با FRAP (636/0=r) و ظرفیت جمعآوری رادیکال نیتریت (721/0=r) مشاهده گردید. میزان ترکیبات فنلی شناسایی شده در عصاره های مختلف از 29/0 میکروگرم برگرم (اسید وانیلیک، پوستۀ ژنوتیپ S5) تا 6/433 میکروگرم بر گرم (سینرژیک اسید، مغز ژنوتیپ S2) متفاوت بود. بنابراین، نتایج نشان داد که مغز دانههای آفتابگردان را میتوان بعنوان ضداکساینده های طبیعی در رژیم غذایی مورد استفاده قرار داد. | ||
| کلیدواژه‌ها [English] | ||
| آفتابگردان, پوسته دانه, ترکیب فنلی, فعالیت ضداکسایشی, مغز دانه | ||
| مراجع | ||
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