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A QTL linkage map of safflower for yield under drought stress at reproductive stage | ||
| Iranian Journal of Genetics and Plant Breeding | ||
| مقاله 9، دوره 4، شماره 2 - شماره پیاپی 8، دی 2015، صفحه 20-27 اصل مقاله (447.18 K) | ||
| نویسندگان | ||
| Manzar Mirzahashemi1؛ Ghasem Mohammadi-Nejad* 2؛ Pooran Golkar3 | ||
| 1Graduated Student of Plant Breeding, College of Agriculture, Shahid Bahonar University of Kerman, Iran. | ||
| 2Associate Professor of Plant Breeding, Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Bahonar University of Kerman, P.O.Box: 76169-133 Kerman, Iran. | ||
| 3Assistant Professor of Plant Breeding, Institute of Biotechnology and Bioengineering, Isfahan University of Technology, 84156 83111 Isfahan, Iran. | ||
| تاریخ دریافت: 21 تیر 1395، تاریخ پذیرش: 21 تیر 1395 | ||
| چکیده | ||
| This study reports QTL mapping for seed yield and its components in safflower genome under drought stress. The F3 families derived from the cross Mex.22-191 (tolerant) × IL.111 (sensitive) were evaluated for agronomic traits in safflower. Drought tolerance was evaluated during 10% of the flowering stage. To identify QTLs underlying tolerance to drought, mapping quantitative trait loci (QTLs) was carried out by composite interval mapping function. A genetic linkage map (LG) assembled from SSR and ISSR markers, was mapped. A total of 145 DNA bands (SSR and ISSR markers) coalesced into 24 LGs which summed to 646 cM in the total map length. This analysis resulted in the identification of 18 QTLs related to seed yield and its components. Based on findings in this study, four major QTLs and three linkage groups (2, 4 and 6) played a crucial role in drought tolerance of safflower. The present linkage map may give a useful framework for mapping agronomic traits in safflower and the framework maps of C. tinctorius can serve as a foundation for future map integration, comparative genomics, QTL analysis and marker assisted breeding for drought tolerance. | ||
| کلیدواژه‌ها | ||
| Drought stress؛ Linkage group؛ QTL analysis؛ Safflower | ||
| عنوان مقاله [English] | ||
| نقشه یابی ژنتیکی گلرنگ برای مکان یابی QTLهای کنترل کننده عملکرد تحت تنش خشکی در مرحله زایشی | ||
| نویسندگان [English] | ||
| منظر میرزاهاشمی1؛ منظر محمدی نژاد2؛ پوران گلکار3 | ||
| 1دانشجوی سابق کارشناسی ارشد اصلاح نباتات، بخش زراعت و اصلاح نباتات، دانشکده کشاورزی ، دانشگاه شهید باهنر کرمان، ایران. | ||
| 2دانشیار اصلاح نباتات، بخش زراعت و اصلاح نباتات، دانشکده کشاورزی ، دانشگاه شهید باهنرکرمان، کرمان، ایران، کد پستی 133-76169. | ||
| 3استادیاراصلاح نباتات، پژوهشکده زیست فناوری، دانشگاه صنعتی اصفهان، اصفهان، ایران. | ||
| چکیده [English] | ||
| در این مطالعه شناسائی QTL های کنترل کننده عملکرد و اجزای عملکرد در ژنوم گلرنگ تحت تنش خشکی صورت گرفت. فامیل های F3 منتج از تلاقی لاین مکزیکی 191-22 ( لاین متحمل به خشکی) و لاین ایرانی IL111 ( لاین حساس) به خشکی از نظر صفات مزرعه ای مورد ارزیابی قرار گرفتند. تحمل به خشکی در مرحله 10 درصد گلدهی اعمال شد. برای شناسائی QTLهای کنترل کننده صفات مرتبط با تحمل به خشکی از روش مکان یابی فاصله ای مرکب استفاده شد. نقشه ژنتیکی با استفاده از نشانگرهای SSR و ISSR استفاده گردید. 145 باند حاصله در قالب 24 گروه لینکاژی طول نقشه ای برابر با 646 سانتی مورگان پوشش دادند. این مطالعه به شناسائی 18 QTL مرتبط با عملکرد و اجزای عملکرد منتهی شد. براساس یافته های این پژوهش چهار QTL بزرگ اثر و سه گروه لینکاژی 2،4و 6 نقش مهمی در تحمل به خشکی نشان دادند. نقشه ژنتیکی حاضر ارائه کننده اطلاعات اولیه مفیدی برای مکان یابی QTLهای صفات زراعی مهم گلرنگ تحت تنش خشکی است و می تواند به عنوان مبنای اولیه در مطالعات نقشه یابی مقایسه ای، اشباع نقشه با هدف گزینش به کمک نشانگر در مطالعات بعدی مورد استفاده قرار گیرد. | ||
| کلیدواژه‌ها [English] | ||
| تنش خشکی, گروه پیوستگی, تجزیه QTL, گلرنگ | ||
| مراجع | ||
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