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High frequency in vitro propagation of M × M60, a cherry rootstock: the effects of culture media and growth regulators | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 10، دوره 4، شماره 2 - شماره پیاپی 8، دی 2015، صفحه 28-36 اصل مقاله (899.48 K) | ||
نویسندگان | ||
Batool Hosseinpour* 1؛ Naser Bouzari2؛ Zahra Didar3؛ Majid Masoumian1؛ Seyed Ali Ghaemmaghami1؛ Asa Ebrahimi3؛ Seyedeh Maryam Mirabbasi1؛ Atefeh Farvardin3 | ||
1Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535111, Tehran, Iran. | ||
2Stone Fruit Research Group, Seed and Plant Improvement Research Institute of Karaj Mahdasht Road, P.O. Box 31585-4119, Karaj , Iran. | ||
3Department of Biotechnology, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran. | ||
تاریخ دریافت: 21 تیر 1395، تاریخ پذیرش: 21 تیر 1395 | ||
چکیده | ||
Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and ME supplemented with 0, 0.5, 0.7, 1 mg L-1 of BAP. As a result, shoot number, average shoot length node number and internode length (except leaf area) were significantly affected by the interaction of medium with BAP level. The largest number of shoots was obtained in DKW (10.3 shoots/explants), ME (9.1 shoots/explants) and QL (9.1 shoots/explants) containing 0.7 mg L-1 BAP. The results showed that DKW with 0.7 mg L-1 BAP had the highest productivity compared to other treatments. For rooting, in vitro proliferated and elongated shoots were planted on LS, MS and ½ MS medium containing 0, 0.5, 1 and 1.5 mg L-1 of IBA. High rooting percentage, root length and root number were obtained on ½ MS supplemented with 1 mg L-1 IBA. The in vitro plantlets were transferred into the soil and the survival rate ex vitro was 95%. | ||
کلیدواژهها | ||
M×M60؛ Cherry rootstock؛ Microprop-agation؛ Rooting؛ BAP | ||
عنوان مقاله [English] | ||
ریزازدیادی پایه رویشی گیلاس M×M60 با راندمان بالا: اثر انواع محیط کشت و تنظیم کنندههای رشد | ||
نویسندگان [English] | ||
بتول حسین پور1؛ ناصر بوذری2؛ زهرا دیدار3؛ مجید معصومیان1؛ سید علی قائم مقامی1؛ آسا ابراهیمی3؛ سیده مریم میرعباسی1؛ عاطفه فروردین3 | ||
1پژوهشکده کشاورزی، سازمان پژوهش های علمی و صنعتی ایران، تهران، کدپستی: 33535111. | ||
2بخش تحقیقات باغبانی، مؤسسۀ تحقیقات علوم باغبانی، کرج، جادۀ محمدشهر، کدپستی: 4119-31585. | ||
3دانشگاه آزاد اسلامی ، واحد علوم تحقیقات، تهران، کدپستی: 775-14515. | ||
چکیده [English] | ||
اثرات محیط های کشت مختلف و تنظیم کننده های رشد روی پرآوری نوساقه و ریشه زایی قلمه های M×M60 مورد مطالعه قرار گرفت. ساقهها در فصل رشد از گیاهان بالغ جدا شده و پس از ضدعفونی در محیط استقرار کشت شدند. بعد از چهار هفته، جوانه های رشد یافته بریده شده و در محیط های پرآوری نوساقه MS ، DKW، QL و ME حاوی 0، 5/0، 7/0 و 1 میلی گرم در لیتر BAP کشت شدند. نتایج نشان داد که به جز سطح برگ، صفات تعداد نوساقهها، میانگین طول نوساقه، تعداد گره و طول میانگره به طور معنی دار تحت تاثیر اثر متقابل محیط کشت و سطوح BAP بود. بیشترین تعداد نوساقه در محیط های DKW (1/10 نوساقه روی هر ریزنمونه)، ME (1/9 نوساقه روی هر نوساقه) و QL (1/9 نوساقه روی هر ریزنمونه) حاوی 7/0 میلی گرم در لیتر BAP بدست آمد. نتایج نشان داد که محیط کشت DKW حاوی 7/0 میلی گرم در لیتر BAP در مقایسه با تیمارهای دیگر بیشترین بازدهی تولید را داشت. به منظور ریشه زایی، نوساقههای طویل شده به محیطهای LS، MS و 1/2MS حاوی 0، 5/0، 1 و 5/1 میلی گرم IBA منتقل شدند. بالاترین درصد ریشه زایی، بلندترین طول ریشه و بیشترین تعداد ریشه در محیط کشت 1/2MS حاوی 1 میلی گرم در لیتر IBA بدست آمد. جهت سازگاری، گیاهچه های ریشه دار شده به خاک منتقل شدند به طوریکه راندمان زنده ماندن 95 درصد بود. | ||
کلیدواژهها [English] | ||
M×M60, پایه رویشی گیلاس, پرآوری نوساقه, ریشه زایی, BAP | ||
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