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The ameliorative effect of silicon and potassium on drought stressed grape (Vitis vinifera L.) leaves | ||
Iranian Journal of Genetics and Plant Breeding | ||
مقاله 12، دوره 4، شماره 2 - شماره پیاپی 8، دی 2015، صفحه 48-58 اصل مقاله (669.63 K) | ||
نویسندگان | ||
Raheem Haddad* 1؛ Alireza Kamangar2 | ||
1Associate Professor, Department of Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran. P. O. Box: 34148-96818. | ||
2MSc, Department of Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran. | ||
تاریخ دریافت: 21 تیر 1395، تاریخ پذیرش: 21 تیر 1395 | ||
چکیده | ||
The effect of sodium silicate (Si) and potassium (K) were investigated on the major antioxidant enzyme activities in two different grapevine cultivars (Vitis vinifera L., cvs Yezandai and Malinger Ramfi) under drought stress. The traits included superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), peroxidase (POD, EC 1.11.1.7), guaiacol peroxidase (GPX, EC 1.11.1.7), ascorbate peroxidase (APX, EC 1.11.1.11), and also physiological traits such as leaf water content ratio (LWCR), chlorophyll (Chl), soluble protein contents, hydrogen peroxide (H2O2), proline (Pro) and glycine betaine (GB) accumulation. The experiment was performed in a completely randomized design including four treatments i.e. the control, drought, Si-drought (0.004 M sodium silicate/kg soil), and K-drought (0.004 M potassium phosphate/kg soil) with three replications in a green house. Drought stress caused a considerable decrease in LWCR, chlorophyll and soluble protein contents. In contrast, compared with the plants treated with drought, applied Si and K significantly enhanced the activities of SOD, CAT, POD, GPX and APX. Under drought stress Pro and GB increased. The results indicated that Si and K offset partially the negative impacts of drought stress by increasing the tolerance of grapevine through rising antioxidant enzyme activities and osmotic adjustment. | ||
کلیدواژهها | ||
Antioxidant enzymes؛ Drought stress؛ Grapevine؛ Potassium؛ Silicon | ||
عنوان مقاله [English] | ||
اثرات بهبود دهندگی سیلیکون و پتاسیم در تنش خشکی برگهای انگور (Vitis vinifera L.) | ||
نویسندگان [English] | ||
رحیم حداد1؛ علیرضا کمانگر2 | ||
1دانشیار گروه بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران. | ||
2کارشناسی ارشد رشته بیوتکنولوژی کشاورزی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران. | ||
چکیده [English] | ||
اثرات سیلیکات سدیم (SI) و پتاسیم (K) در فعالیت آنزیم های آنتی اکسیدان مهم در دو رقم مختلف انگور (Vitis vinifera L.) یزندایی و رامفی تحت تنش خشکی مورد بررسی قرار گرفتند. صفات مورد بررسی شامل سوپراکسید دیسموتاز (SOD, EC 1.15.1.1)، کاتالاز EC 1.15.1.6) (CAT,، پراکسیداز EC 1.11.1.7) (POD,، گوایکول پراکسیداز (GPX, EC1.11.1.7)، آسکوربات پراکسیداز(APX, EC 1.11.1.11) و همچنین صفات فیزیولوژیک مانند نسبت مقدار آب برگ (LWCR)، کلروفیل(Chl) ، پروتئین محلول در آب، پراکسید هیدروژن(H2O2) ، پرولین (Pro) و تجمع گلایسین بتائین (GB) بودند. این آزمایش در قالب طرح کاملاً تصادفی شامل چهار تیمار کنترل، خشکی، سیلیکون- خشکی (004/0 مولار سیلیکات سدیم / کیلوگرم خاک)، و پتاسیم- خشکی (004/0 مولار فسفات پتاسیم / کیلوگرم خاک) با سه تکرار در گلخانه انجام شد. تنش خشکی باعث کاهش قابل توجهی در LWCR، کلروفیل و پروتئین محلول گردید. در مقابل، در مقایسه با گیاهان تحت تیمار خشکی، اعمال Si و K به طور معنیداری فعالیت SOD، CAT، POD،GPX و APX را افزایش داد. تحت تنش خشکی مقادیر Pro و GB افزایش یافت. نتایج نشان داد که کاربرد Si و K تا حدودی اثرات منفی تنش خشکی را با افزایش تحمل انگور از طریق فعالیت آنزیم های آنتی اکسیدانت و تنظیم فشار اسمزی جبران مینماید. | ||
کلیدواژهها [English] | ||
آنزیمهای آنتی اکسیدانت, تنش خشکی, انگور, پتاسیم, سیلیکون | ||
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