اخبار و اعلانات
آمار
| تعداد نشریات | 20 |
| تعداد شماره‌ها | 347 |
| تعداد مقالات | 2,862 |
| تعداد مشاهده مقاله | 3,253,625 |
| تعداد دریافت فایل اصل مقاله | 2,389,228 |
تأثیر روش های مختلف استخراج بر متابولیت ها و خواص بیولوژیکی بذر گیاه چویل Ferulago angulate | ||
| پژوهش های پیشرفته در گیاهان دارویی | ||
| دوره 1، شماره 1، دی 1401، صفحه 1-10 اصل مقاله (864.53 K) | ||
| نوع مقاله: مقاله علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30479/armp.2022.17249.1000 | ||
| نویسندگان | ||
| سعید ملائی* 1؛ فرزانه صدیقی2؛ سعید حضرتی3 | ||
| 1استادیار، گروه شیمی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز | ||
| 2دانشجوی کارشناسی ارشد، گروه شیمی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز | ||
| 3دانشیار، هیات علمی، گروه گیاهان داروئی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز | ||
| تاریخ دریافت: 24 اردیبهشت 1401، تاریخ بازنگری: 16 مهر 1401، تاریخ پذیرش: 16 مهر 1401 | ||
| چکیده | ||
| گیاه چویل با نام علمی Ferulago angulata متعلق به تیره چتریان (Apiaceae) و از گیاهان دارویی با ارزش و بومی است که در غرب و جنوب غرب ایران پراکنش زیادی دارد. این گیاه از گذشته‌های دور به عنوان داروی آرام‌بخش و گوارشی است و در زمینه‌های مختلف از جمله درمان‌کننده کرم‌های روده‌ای، آنتی‌اکسیدان، ضدمیکروبی، ضدسرطان، درمان فشارخون، آلزایمر و دیابت کاربرد دارد. در این مطالعه، تأثیر روش‌های مختلف استخراج از جمله التراسونیک، هم‌زدن، خیساندن، سوکسله، رفلاکس و میکروویو بر محتوای ترکیبات فنلی و فعالیت آنتی‌اکسیدانی عصاره متانولی مورد بررسی قرار گرفت. میزان فنل و فلاونوئید کل با استفاده از روش‌های اسپکتروفتومتری و عملکرد آنتی‌اکسیدانی عصاره‌‌ها نیز با استفاده از روش DPPH اندازه گیری شد. با توجه به نتایج بدست آمده، عصاره بدست آمده از روش سوکسله دارای بیشترین بازده بود. بیشترین مقدار فنل کل (mg GAE/g extract 77/26) و فلاونوئید کل (mg QE/g extract 82/5) به ترتیب به عصاره حاصل از هم‌زدن و سوکسله اختصاص داشت. بیشترین میزان عملکرد آنتی‌اکسیدانی متعلق به عصاره سوکسله و رفلاکس با میزان IC50= 59 µg/mL در مهار رادیکال آزاد گزارش شد. بررسی نتایج نشان داد که روش‌های استخراج عصاره، نقش مهمی در کمیت و خاصیت آنتی‌اکسیدانی مواد مؤثر گیاهان ایفا می‌کند. در نتیجه، روش سوکسله بهترین روش عصاره‌گیری به منظور افزایش بازده ترکیبات مؤثر گیاه چویل بود. | ||
| کلیدواژه‌ها | ||
| آنتی‌اکسیدان؛ التراسونیک؛ سوکسله؛ فنل؛ گیاه چویل؛ میکروویو | ||
| عنوان مقاله [English] | ||
| Effect of different extraction methods on the metabolites and biological activities of chaville seeds (Ferulago angulate) | ||
| نویسندگان [English] | ||
| Saeed Mollaei1؛ Farzaneh Sedighi2؛ Saeed Hazrati3 | ||
| 1Assistant Professor, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran | ||
| 2M.Sc. Student, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran | ||
| 3Associate Professor, Department of Agronomy, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran | ||
| چکیده [English] | ||
| Chaville (Ferulago angulate) belonging to the Apiaceae family, is a native medicinal plant which is distributed in the west and south west regions of Iran. It is used as a sedative and digestive drug since ancient time and is consumed to treat intestinal worms, antioxidants, antimicrobials, anticancer drugs, blood pressure treatments, and Alzheimer’s and diabetes effects. In this study, the effects of different extraction methods including ultrasound, agitation, maceration, soxhlet, reflux and microwave were investigated on the content of phenolic compounds and antioxidant activity of methanolic extract. Determination of total phenol and flavonoid contents were determined using spectrophotometric methods and antioxidant activity of the extracts were also assayed by DPPH method. According to the results, the extract obtained by soxhlet method had the highest yield. Also, the highest total phenol content (26.77 mg GAE/ g extract) belonged to the agitation method, while the highest total flavonoid content (5.82 mg QE/ g extract) was reported by the soxhlet method. The highest antioxidant activity was achieved at the extract obtained by soxhlet and reflux methods with IC50 = 59 μg/mL. The results indicated that extraction methods play an important role in the quality and antioxidant activity of active compounds. In conclusion, soxhlet method was the best method in order to increase the yield of active compounds at Ferulago angulate. | ||
| کلیدواژه‌ها [English] | ||
| Antioxidant, Ferulago angulate, Microwave, Phenol, Soxhlet, Ultrasonic | ||
| مراجع | ||
|
Amirghofran Z, Bahmani M, Azadmehr A, Javidnia K, Miri R. 2009. Immunomodulatory activities of various medicinal plant extracts: effects on human lymphocytes apoptosis. Iranian Journal of Science and Technology, Transactions A 38: 181-192. Azmir J, Zaidul ISM, Rahman MM, Sharif KM, Mohamed A, Sahena F, Omar AKM. 2013. Techniques for extraction of bioactive compounds from plant materials: a review. Journal of Food Engineering 117 (4): 426-436. Ghasemzadeh A, Jaafar H, Juraimi A, Tayebi-Meigooni A. 2015. Comparative evaluation of different extraction techniques and solvents for the assay of phytochemicals and antioxidant activity of hashemi rice bran. Molecules 20 (6): 10822-10838. Govahi R, Ghalamkari G, Toghyani M, Eghbal Saied S, Mohammadrezaei M, Shahryari M, Dehghani Abari A. 2013. Effect of Ferulago angulata sub. carduchorum on total serum antioxidant activity and some of the humoral immune responses in broiler chicks. Journal of Herbal Drugs (An International Journal on Medicinal Herbs) 4(3): 119-126. Haghighi Z, Modarresi M, Mollayi S. 2012. Enhancement of compatible solute and secondary metabolites production in Plantago ovata Forsk. by salinity stress. Journal of Medicinal Plants Research 6 (18): 3495-3500. Heidari S, Akrami H, Gharaei R, Jalili A, Mahdiuni H, Golezar E. 2014. Anti-tumor activity of Ferulago angulata Boiss. Extract in gastric cancer cell line via induction of apoptosis. Iranian Journal of Pharmaceutical Research 13(4): 1335-1345. Karabegović IT, Stojičević SS, Veličković DT, Todorović ZB, Nikolić NČ, Lazić ML. 2014. The effect of different extraction techniques on the composition and antioxidant activity of cherry laurel (Prunus laurocerasus) leaf and fruit extracts. Industrial Crops and Products 54: 142-148. Khanahmadi M, Janfeshan K. 2006. Study on antioxidation property of Ferulago angulate plant. Asian Journal of Plant Sciences 5 (3): 521-526. Kiziltas H, Ekin S, Bayramoglu M, Akbas E, Oto G, Yildirim S, Ozgokce F. 2017. Antioxidant properties of Ferulago angulata and its hepatoprotective effect against N-nitrosodimethylamine-induced oxidative stress in rats. Pharmaceutical Biology 55 (1): 888-897. Leopoldini M, Marino T, Russo N, Toscano M. 2004. Antioxidant properties of phenolic compounds: H-atom versus electron transfer mechanism. The Journal of Physical Chemistry A 108 (22): 4916-4922. Li Z, Lee HW, Liang X, Liang D, Wang Q, Huang D, Ong CN. 2018. Profiling of phenolic compounds and antioxidant activity of 12 cruciferous vegetables. Molecules 23 (5): E1139. Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X, Chen H. 2016. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules 21 (10): 1374. Lorigooini Z, Koravand M, Haddadi H, Rafieian-Kopaei M, Shirmardi HA, Hosseini Z. 2019. A review of botany, phytochemical and pharmacological properties of Ferulago angulata. Toxin Reviews 38 (1): 13-20. Mollaei S, Habibi B, Amani Ghadim A, Shakouri M. 2018. A green approach for the synthesis of silver nanoparticles using Lithospermum officinale root extract and evaluation of their antioxidant activity. Journal of Particle Science & Technology 3 (4): 187-195. Mozaffarian V. 2008. Flora of Ilam. Farhang Moaser Publication, Tehran, Iran. Naghiha R, Keshtkaran A, Hosseini H. 2015. Effects of ethanolic Chavill extract on growth of lactobacillus and salmonella bacteria, in skimmed milk and imaging gastric-intestine media in vitro. Armaghane Danesh 20 (5): 393-403. Nile SH, Nile AS, Keum YS. 2017. Total phenolics, antioxidant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods. 3 Biotech 7 (1): 76. Nimse SB, Pal D. 2015. Free radicals, natural antioxidants, and their reaction mechanisms. Rsc Advances 5 (35): 27986-28006. Oniszczuk A, Podgórski R, Oniszczuk T, Żukiewicz-Sobczak W, Nowak R, Waksmundzka-Hajnos M. 2014. Extraction methods for the determination of phenolic compounds from Equisetum arvense L. herb. Industrial Crops and Products 61: 377-381. Perron NR, Brumaghim JL. 2009. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochemistry and Biophysics 53 (2): 75-100. Rustaiyan A, Sedaghat S, Larijani K, Khossravi M, Masoudi S. 2002. Composition of the essential oil of Ferulago angulata (Schlecht.) Boiss. from Iran. Journal of Essential Oil Research 14 (6): 447-448. Sadeghi E, Mahtabani A, Etminan A, Karami F. 2016. Stabilization of soybean oil during accelerated storage by essential oil of Ferulago angulate boiss. Journal of Food Science and Technology 53 (2): 1199-1204. Sodeifian G, Ansari K, Bamoniri A, Mirjalili B. 2011. Study of chemical composition of the essential oil of Ferulago angulata (Schelcht) Boiss. from Iran using supercritical fluid extraction and nanoscale injection. Digest Journal of Nanomaterials and Biostructures 6: 161-168. Starmans DA, Nijhuis HH. 1996. Extraction of secondary metabolites from plant material: a review. Trends in Food Science & Technology 7 (6): 191-197. Tamuly C, Saikia B, Hazarika M, Bora J, Bordoloi MJ, Sahu OP. 2013. Correlation between phenolic, flavonoid, and mineral contents with antioxidant activity of underutilized vegetables. International Journal Vegetable Science 19 (1): 34-44. Varela MC. 2016. Phenolic compounds: natural antioxidants, other benefices and future perspectives. Austin Biomol Open Access 1 (1): 1001. Vega Arroy JD, Ruiz-Espinosa H, Luna-Guevara JJ, Luna-Guevara ML, Hernández-Carranza P, Ávila-Sosa R, Ochoa-Velasco CE. 2017. Effect of solvents and extraction methods on total anthocyanins, phenolic compounds and antioxidant capacity of Renealmia alpinia (Rottb.) maas peel. Czech Journal of Food Science 35 (5): 456-465. Xiong Y, Zhou L, Zhao Y, Liu Y, Liu X, Zhu G, Liu Z. 2018. Effects of different extraction methods on the extraction rates of five chemical ingredients of Swertia mussotii Franch by UPLC-ESI-MS/MS. In IOP Conference Series: Materials Science and Engineering 301 (1): 120-24. Yenge GB, More HG, Kanawade RKV, Nimbalkar CA, Patil AP. 2017. Effect of different extraction methods on yield and physico-chemical properties of garden cress (Lepidium sativam L.) oil. Journal of Oilseed Brassica 81 (2): 138-142. | ||
|
آمار تعداد مشاهده مقاله: 276 تعداد دریافت فایل اصل مقاله: 292 |
||