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تأثیر روش های مختلف استخراج بر متابولیت ها و خواص بیولوژیکی بذر گیاه چویل Ferulago angulate | ||
| پژوهش های پیشرفته در گیاهان دارویی | ||
| دوره 1، شماره 1، دی 1401، صفحه 1-10 اصل مقاله (864.53 K) | ||
| نوع مقاله: مقاله علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30479/armp.2022.17249.1000 | ||
| نویسندگان | ||
| سعید ملائی* 1؛ فرزانه صدیقی2؛ سعید حضرتی3 | ||
| 1استادیار، گروه شیمی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز | ||
| 2دانشجوی کارشناسی ارشد، گروه شیمی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز | ||
| 3دانشیار، هیات علمی، گروه گیاهان داروئی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز | ||
| تاریخ دریافت: 24 اردیبهشت 1401، تاریخ بازنگری: 16 مهر 1401، تاریخ پذیرش: 16 مهر 1401 | ||
| چکیده | ||
| گیاه چویل با نام علمی Ferulago angulata متعلق به تیره چتریان (Apiaceae) و از گیاهان دارویی با ارزش و بومی است که در غرب و جنوب غرب ایران پراکنش زیادی دارد. این گیاه از گذشته‌های دور به عنوان داروی آرام‌بخش و گوارشی است و در زمینه‌های مختلف از جمله درمان‌کننده کرم‌های روده‌ای، آنتی‌اکسیدان، ضدمیکروبی، ضدسرطان، درمان فشارخون، آلزایمر و دیابت کاربرد دارد. در این مطالعه، تأثیر روش‌های مختلف استخراج از جمله التراسونیک، هم‌زدن، خیساندن، سوکسله، رفلاکس و میکروویو بر محتوای ترکیبات فنلی و فعالیت آنتی‌اکسیدانی عصاره متانولی مورد بررسی قرار گرفت. میزان فنل و فلاونوئید کل با استفاده از روش‌های اسپکتروفتومتری و عملکرد آنتی‌اکسیدانی عصاره‌‌ها نیز با استفاده از روش DPPH اندازه گیری شد. با توجه به نتایج بدست آمده، عصاره بدست آمده از روش سوکسله دارای بیشترین بازده بود. بیشترین مقدار فنل کل (mg GAE/g extract 77/26) و فلاونوئید کل (mg QE/g extract 82/5) به ترتیب به عصاره حاصل از هم‌زدن و سوکسله اختصاص داشت. بیشترین میزان عملکرد آنتی‌اکسیدانی متعلق به عصاره سوکسله و رفلاکس با میزان IC50= 59 µg/mL در مهار رادیکال آزاد گزارش شد. بررسی نتایج نشان داد که روش‌های استخراج عصاره، نقش مهمی در کمیت و خاصیت آنتی‌اکسیدانی مواد مؤثر گیاهان ایفا می‌کند. در نتیجه، روش سوکسله بهترین روش عصاره‌گیری به منظور افزایش بازده ترکیبات مؤثر گیاه چویل بود. | ||
| کلیدواژه‌ها | ||
| آنتی‌اکسیدان؛ التراسونیک؛ سوکسله؛ فنل؛ گیاه چویل؛ میکروویو | ||
| عنوان مقاله [English] | ||
| Effect of different extraction methods on the metabolites and biological activities of chaville seeds (Ferulago angulate) | ||
| نویسندگان [English] | ||
| Saeed Mollaei1؛ Farzaneh Sedighi2؛ Saeed Hazrati3 | ||
| 1Assistant Professor, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran | ||
| 2M.Sc. Student, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran | ||
| 3Associate Professor, Department of Agronomy, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran | ||
| چکیده [English] | ||
| Chaville (Ferulago angulate) belonging to the Apiaceae family, is a native medicinal plant which is distributed in the west and south west regions of Iran. It is used as a sedative and digestive drug since ancient time and is consumed to treat intestinal worms, antioxidants, antimicrobials, anticancer drugs, blood pressure treatments, and Alzheimer’s and diabetes effects. In this study, the effects of different extraction methods including ultrasound, agitation, maceration, soxhlet, reflux and microwave were investigated on the content of phenolic compounds and antioxidant activity of methanolic extract. Determination of total phenol and flavonoid contents were determined using spectrophotometric methods and antioxidant activity of the extracts were also assayed by DPPH method. According to the results, the extract obtained by soxhlet method had the highest yield. Also, the highest total phenol content (26.77 mg GAE/ g extract) belonged to the agitation method, while the highest total flavonoid content (5.82 mg QE/ g extract) was reported by the soxhlet method. The highest antioxidant activity was achieved at the extract obtained by soxhlet and reflux methods with IC50 = 59 μg/mL. The results indicated that extraction methods play an important role in the quality and antioxidant activity of active compounds. In conclusion, soxhlet method was the best method in order to increase the yield of active compounds at Ferulago angulate. | ||
| کلیدواژه‌ها [English] | ||
| Antioxidant, Ferulago angulate, Microwave, Phenol, Soxhlet, Ultrasonic | ||
| مراجع | ||
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