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بررسی اثر عصاره هیدروالکلی گیاهان زیره سبز و زنجبیل به صورت مجزا و توأمان بر ردههای سلولی سرطان پستان MDA-MB-231 و MCF-7 | ||
پژوهش های پیشرفته در گیاهان دارویی | ||
دوره 1، شماره 1، دی 1401، صفحه 37-48 اصل مقاله (1.2 M) | ||
نوع مقاله: مقاله علمی- پژوهشی | ||
شناسه دیجیتال (DOI): 10.30479/armp.2023.17911.1005 | ||
نویسندگان | ||
خسرو حسینی پژوه* 1؛ مهدیه عبدالله2 | ||
1استادیار، پژوهشکده بیوتکنولوژی، سازمان پژوهش های علمی و صنعتی ایران، تهران | ||
2کارشناسی ارشد، گروه ژنتیک سلولی، واحد علوم تحقیقات، دانشگاه آزاد اسلامی، تهران | ||
تاریخ دریافت: 03 آبان 1401، تاریخ بازنگری: 10 دی 1401، تاریخ پذیرش: 18 دی 1401 | ||
چکیده | ||
هدف از این مطالعه، بررسی اثر سمیت عصاره زیره سبز و زنجبیل بر ردههای سلولی سرطان پستان MDA-MB-231 و MCF-7 و مقایسه با سلولهای RHDP و Vero به عنوان سلولهای طبیعی است. عصارههای دو گیاه به محیط این 4 رده سلول اضافه، و پس از 72 ساعت، زندهمانی سلولها به روش MTT ارزیابی شد. اثر تجمعی دو عصاره با غلظت 100 میکروگرم بر میلیلیتر زیره سبز و غلظتهای مختلف زنجبیل بررسی گردید. برای تعیین میزان آپوپتوز رنگآمیزی AnnexinV-FITC/PI انجام شد. در سلولهای MDA، IC50 برای عصاره زیره µg/ml4/107 و عصاره زنجبیل µg/ml8/158 و در رده سلولی MCF-7، IC50 برای عصاره زیره µg/ml4/197 و زنجبیل µg/ml4/120 بود. عصاره زنجبیل و زیره در سلولهای غیر سرطانی VERO و RHDP تا µg/ml200 باعث افزایش میزان زندهمانی این سلولها شد. در سلولهای VERO و RHDP، IC50 حدود µg/ml700 یا بالاتر بود. استفاده توأمان این دو عصاره موجب کاهش غلظت ایجاد کننده IC50 از هر دو گیاه شد. رنگآمیزی با AnnexinV-FITC/PI نشان داد که عصاره دو گیاه موجب القای آپوپتوز در 28 درصد سلولها بعد از 36 ساعت شد (در مقابل 5/10 درصد در کنترل). بنابراین زیره و زنجبیل بر رده سلولهای سرطانی در مقادیری بیضرر برای سلولهای طبیعی میتواند باعث مهار تکثیر سلولهای سرطانی شود. | ||
کلیدواژهها | ||
زنجبیل؛ زیره؛ سرطان پستان؛ ضد سرطان | ||
عنوان مقاله [English] | ||
The effect of hydroalcoholic extract of cumin and ginger plants separately and combined on MDA-MB-231 and MCF-7 breast cancer cell lines | ||
نویسندگان [English] | ||
Khosro Hoseini Pajooh1؛ Mahdieh Abdollah2 | ||
1Assistant Professor, Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran | ||
2M.Sc., Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
چکیده [English] | ||
This study investigates the toxicity effect of cumin and ginger extracts on breast cancer cell lines MDA-MB-231 and MCF-7 and compare them with RHDP and Vero as normal cells. The extracts of two plants were added to the medium of these 4 cell lines and after 72 h, cell viability was measured by the MTT method. The cumulative effect of two extracts with a concentration of 100 μg/ml of cumin and different concentrations of ginger extract was investigated. AnnexinV-FITC/PI staining was carried out to determine the level of apoptosis. In MDA cells, the IC50 of cumin and ginger were 107.4 µg/ml and 158.8 µg/ml, and in the MCF-7 cell line, the IC50 of cumin and ginger were 197.4 µg/ml and 120.4 µg/ml, respectively. Ginger and cumin extracts in VERO and RHDP cells increased the survival rate of these cells, up to 200 µg/ml. The IC50 in these cells was around 700 µg/ml or higher. The combined use of these two extracts decreased the IC50 concentration of both plants. In AnnexinV-FITC/PI staining, a total of about 28 % of the cells under the influence of two extracts became apoptotic after 36 h (vs 10.5 % in control). Therefore, cumin and ginger extracts in harmless titers for normal cells can inhibit the proliferation of cancerous cells. | ||
کلیدواژهها [English] | ||
Anti-Cancer, Breast Cancer, Cumin, Ginger | ||
مراجع | ||
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