اخبار و اعلانات
آمار
| تعداد نشریات | 19 |
| تعداد شماره‌ها | 380 |
| تعداد مقالات | 3,131 |
| تعداد مشاهده مقاله | 4,251,749 |
| تعداد دریافت فایل اصل مقاله | 2,846,083 |
بررسی اثر عصاره هیدروالکلی گیاهان زیره سبز و زنجبیل به صورت مجزا و توأمان بر رده‌های سلولی سرطان پستان MDA-MB-231 و MCF-7 | ||
| پژوهش های پیشرفته در گیاهان دارویی | ||
| دوره 1، شماره 1، دی 1401، صفحه 37-48 اصل مقاله (1.2 M) | ||
| نوع مقاله: مقاله علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30479/armp.2023.17911.1005 | ||
| نویسندگان | ||
| خسرو حسینی پژوه* 1؛ مهدیه عبدالله2 | ||
| 1استادیار، پژوهشکده بیوتکنولوژی، سازمان پژوهش های علمی و صنعتی ایران، تهران | ||
| 2کارشناسی ارشد، گروه ژنتیک سلولی، واحد علوم تحقیقات، دانشگاه آزاد اسلامی، تهران | ||
| تاریخ دریافت: 03 آبان 1401، تاریخ بازنگری: 10 دی 1401، تاریخ پذیرش: 18 دی 1401 | ||
| چکیده | ||
| هدف از این مطالعه، بررسی اثر سمیت عصاره زیره سبز و زنجبیل بر رده‌های سلولی سرطان پستان MDA-MB-231 و MCF-7 و مقایسه با سلول‌های RHDP و Vero به عنوان سلول‌های طبیعی است. عصاره‌های دو گیاه به محیط این 4 رده سلول اضافه، و پس از 72 ساعت، زنده‌مانی سلول‌ها به روش MTT ارزیابی شد. اثر تجمعی دو عصاره با غلظت 100 میکروگرم بر میلی‌لیتر زیره سبز و غلظت‌های مختلف زنجبیل بررسی گردید. برای تعیین میزان آپوپتوز رنگ‌آمیزی AnnexinV-FITC/PI انجام شد. در سلول‌های MDA، IC50 برای عصاره زیره µg/ml4/107 و عصاره زنجبیل µg/ml8/158 و در رده سلولی MCF-7، IC50 برای عصاره زیره µg/ml4/197 و زنجبیل µg/ml4/120 بود. عصاره زنجبیل و زیره در سلول‌های غیر سرطانی VERO و RHDP تا µg/ml200 باعث افزایش میزان زنده‌مانی این سلول‌ها شد. در سلول‌های VERO و RHDP، IC50 حدود µg/ml700 یا بالاتر بود. استفاده توأمان این دو عصاره موجب کاهش غلظت ایجاد کننده IC50 از هر دو گیاه شد. رنگ‌آمیزی با AnnexinV-FITC/PI نشان داد که عصاره دو گیاه موجب القای آپوپتوز در 28 درصد سلول‌ها بعد از 36 ساعت شد (در مقابل 5/10 درصد در کنترل). بنابراین زیره و زنجبیل بر رده سلول‌های سرطانی در مقادیری بی‌ضرر برای سلول‌های طبیعی می‌تواند باعث مهار تکثیر سلول‌های سرطانی شود. | ||
| کلیدواژه‌ها | ||
| زنجبیل؛ زیره؛ سرطان پستان؛ ضد سرطان | ||
| عنوان مقاله [English] | ||
| The effect of hydroalcoholic extract of cumin and ginger plants separately and combined on MDA-MB-231 and MCF-7 breast cancer cell lines | ||
| نویسندگان [English] | ||
| Khosro Hoseini Pajooh1؛ Mahdieh Abdollah2 | ||
| 1Assistant Professor, Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran | ||
| 2M.Sc., Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| This study investigates the toxicity effect of cumin and ginger extracts on breast cancer cell lines MDA-MB-231 and MCF-7 and compare them with RHDP and Vero as normal cells. The extracts of two plants were added to the medium of these 4 cell lines and after 72 h, cell viability was measured by the MTT method. The cumulative effect of two extracts with a concentration of 100 μg/ml of cumin and different concentrations of ginger extract was investigated. AnnexinV-FITC/PI staining was carried out to determine the level of apoptosis. In MDA cells, the IC50 of cumin and ginger were 107.4 µg/ml and 158.8 µg/ml, and in the MCF-7 cell line, the IC50 of cumin and ginger were 197.4 µg/ml and 120.4 µg/ml, respectively. Ginger and cumin extracts in VERO and RHDP cells increased the survival rate of these cells, up to 200 µg/ml. The IC50 in these cells was around 700 µg/ml or higher. The combined use of these two extracts decreased the IC50 concentration of both plants. In AnnexinV-FITC/PI staining, a total of about 28 % of the cells under the influence of two extracts became apoptotic after 36 h (vs 10.5 % in control). Therefore, cumin and ginger extracts in harmless titers for normal cells can inhibit the proliferation of cancerous cells. | ||
| کلیدواژه‌ها [English] | ||
| Anti-Cancer, Breast Cancer, Cumin, Ginger | ||
| مراجع | ||
|
Al-Ataby IA, Talib WH. 2022. Daily consumption of lemon and ginger herbal infusion caused tumor regression and activation of the immune system in a mouse model of breast cancer. Frontiers in Nutrition 9: 829101. Antunes L, de Lourdes Pires Bianchi M. 2010. Chapter 116 - In vivo cytogenetic effects of multiple doses of dietary vegetable oils: position of olive oils. Editor(s): Preedy VR, Watson RR., olives and olive oil in health and disease prevention 1071-1077. Bener A, Alsulaiman R, Doodson L, Agathangelou T. 2017. Depression, hopelessness and social support among breast cancer patients: in highly endogamous population. Asian Pacific Journal of Cancer Prevention (APJCP) 18 (7): 1889-1896. Bray F, Ferlay J, Laversanne M, Brewster DH, Gombe Mbalawa C, Kohler B, et al. 2015. Cancer incidence in five continents: inclusion criteria, highlights from Volume X and the global status of cancer registration. International Journal of Cancer 137 (9): 2060-2071. Chang WS, Chang YH, Lu FJ, Chiang HC. 1994. Inhibitory effects of phenolics on xanthine oxidase. Anticancer Research 14 (2A): 501-506. Curado MP, Edwards B, Shin HR, Storm H, Ferlay J, Heanue M, Boyle P. 2007. Cancer incidence in five continents Volum IX. IARC Scientific Publication No. 160, International Agency for Research on Cancer, Lyon, France. Derakhshanfar A, Niayesh A, Abbasi M, Ghalaeeha A, Shojaee M. 2013. Frequency of depression in breast cancer patients: a study in farshchian and besat hospitals of hamedan during 2007-2008. Iranian Journal of Surgery 21 (2): 68-74. Dinparvar S, Bagirova M, Allahverdiyev AM, Abamor ES, Safarov T, Aydogdu M, Aktas D. 2020. A nanotechnology-based new approach in the treatment of breast cancer: Biosynthesized silver nanoparticles using Cuminum cyminum L. seed extract. Journal of Photochemistry and Photobiology B: Biology 208: 111902. Dumont N, Arteaga CL. 2003. Targeting the TGF beta signaling network in human neoplasia. Cancer Cell 3 (6): 531-536. Editorial. 2009. Breast cancer in developing countries. Lancet (London, England) 374 (9701): 1567. EnayatRad M, Salehinia H. 2015. An investigation of changing patterns in breast cancer incidence trends among Iranian women. Quarterly Journal of Sabzevar University of Medical Sciences 22 (1): 27-35. (In Farsi( Fayazfar S, Zamanyan Azodi M, Heydari Kashel S, Azizye Jalileyan F. 2013. Evaluation of the ctytotoxic effect of Cuminum cyminum essential oils on human colon cancer cell line SW742. Journal of Ilam University of Medical Sciences 20 (5): 138-148. (In Farsi) Fitzpatrick LR, Woldemariam T. 2017. Small-molecule drugs for the treatment of inflammatory bowel disease. Editor(s): Chackalamannil S, Rotella D, Ward SE. Comprehensive Medicinal Chemistry III. Elsevier 495-510. ISBN: 978-0-12-803201-5. Forouzandeh F, Salimi S, Naghsh N, Zamani N, Jahani S. 2014. Evaluation of anti-cancer effect of Peganum harmala L. hydroalcholic extract on human cervical carcinoma epithelial cell line. Journal of Shahrekord Uuniversity of Medical Sciences 16 (4): 1-8. (In Farsi) Gachkar L, Yadegari D, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I. 2007. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chemistry 102: 898-904. Hoveizi E, Mohammadi T. 2018. Comparison of ginger (Zingiber officinale) hydroalcoholic extract on the viability of cancer cells and embryonic fibroblast cells. Journal of Developmental Biology 10 (3): 73-80. (In Farsi) Iacobellis NS, Cantore PL, Capasso F, Senatore F. 2005. Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils. Journal of Agricultural and Food Chemistry 53: 57-61. Jafari A, Goudarzian AH, Bagheri Nesami M. 2018. Depression in women with breast cancer: a systematic review of cross-sectional studies in Iran. Asian Pacific Journal of Cancer Prevention (APJCP) 19 (1): 1-7. Kamkar A, Shariatifar N, Jamshidi AH, Mohammadian M. 2010. Study of antioxidant functional of the water, methanol and ethanol extracts of endemic Cuminum cyminum L. and Cardaria draba L. in the In-vitro Systems. Internal Medicine Today 16(2): 37-44. Lei D, Hong T, Li L, Chen L, Luo X, Wu Q, Liu Z. 2021. Isobaric tags for relative and absolute quantitation-based proteomics analysis of the effect of ginger oil on bisphenol A-induced breast cancer cell proliferation. Oncology Letters 21 (2): 101. Madanipour M, Sharifi A. 2019. Evaluation of chemical composition and antioxidant activity of Cydonia oblonga extract. Innovation in Food Science and Technology, Journal of Food Science and Technology 10 (1): 109-119. Masuda Y, Kikuzaki H, Hisamoto M, Nakatani N. 2004. Antioxidant properties of gingerol related compounds from ginger. Biofactors 21 (1-4): 293-296. Moheghi N, Afshari JT, Brook A. 2011. The cytotoxic effect of Zingiber Afficinale in breast cancer (MCF7) cell line. Quarterly of Horizon of Medical Sciences 17 (3): 28-34. Motavalizadeh Ardekani A, Hashemi M, Safakish M, Alem Bagheri A, Baradaran Shokoohi S, Mosaddegh M. 2012. Medical treatment of cancer in traditional Iranian medicine. Journal of Islamic and Iranian Traditional Medicine 3 (1): 3-18. Nostro A, Cellini L, Di Bartolomeo S, Di Campli E, Grande R, Cannatelli MA, Marzio L, Alonzo V. 2005. Antibacterial effect of plant extracts against Helicobacter pylori. Phytotherapy Research 19 (3): 198-202. Pilevarzadeh M, Amirshahi M, Afsargharehbagh R, Rafiemanesh H, Hashemi SM, Balouchi A. 2019. Global prevalence of depression among breast cancer patients: a systematic review and meta-analysis. Breast Cancer Research and Treatment 176 (3): 519-533. Porter P. 2008. “Westernizing” women’s risks? Breast cancer in lower-income countries. The New England Journal of Medicine 358 (3): 213-216. Prakash E, Kumar Gupta D. 2014. Cytotoxic activity of ethanolic extract of Cuminum cyminum Linn against seven human cancer cell line. Universal Journal of Agricultural Research 2 (1): 27-30. Purohit P, Mustafa M, Osmani Z. 1983. Insecticidal properties of plant-extract of Cuminum cyminum Linn. Scientific Culture 49: 46-49. Remesh A. 2017. Toxicities of anticancer drugs and its management. International Journal of Basic & Clinical Pharmacology 1 (1): 2-12. Siegel RL, Miller KD, Jemal A. 2019. CA: a cancer journal for clinicians. Cancer Statistics 69 (1): 7-34. Sosa V, Moliné T, Somoza R, Paciucci R, Kondoh H, LLeonart ME. 2013. Oxidative stress and cancer: an overview. Ageing Research Reviews 12 (1): 376-390. Kintzios SE. 2006. Terrestrial plant-derived anticancer agents and plant species used in anticancer research. Critical Reviews in Plant Sciences 25 (2): 79-113. Tavakol Afshari J, Moheghi N, Brouk A. 2010. Ethanolic extract cytotoxic effect of Zingiber officinale in hepatocellular carcinoma (hepg2) cell line. Avicenna Journal of Clinical Medicine 17 (3): 52-56. (In Farsi) WHO. International Agency for Research on Cancer 2020. The Global Cancer Observatory. Globocan 2018. Breast. 2019 [Updated 2019 March]. Available from: http://gco.iarc.fr/today/data/factsheets/cancers/20-Breast-fact-sheet.pdf. Willatgamuwa S, Platel K, Saraswathi G, Srinivasan K. 1998. Antidiabetic influence of dietary cumin seeds (Cuminum cyminum) in streptozotocin induced diabetic rats. Nutrition Research 18: 131-142. Zhao L, Rupji M, Choudhary I, Osan R, Kapoor S, Zhang H-J, Yang Ch, Aneja R. 2020. Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer. Scientific Reports 10 (1): 19182. | ||
|
آمار تعداد مشاهده مقاله: 358 تعداد دریافت فایل اصل مقاله: 325 |
||