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Association mapping of morpho-physiological traits in bread wheat under drought-stressed and non-stressed conditions | ||
Iranian Journal of Genetics and Plant Breeding | ||
دوره 11، شماره 1 - شماره پیاپی 21، مرداد 2022، صفحه 35-52 اصل مقاله (975.64 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.30479/ijgpb.2023.18854.1343 | ||
نویسنده | ||
Farzad Ahakpaz* | ||
Department of Agronomy and Plant Breeding, Miandoab Branch, Islamic Azad University, Miandoab, Iran. | ||
تاریخ دریافت: 10 خرداد 1402، تاریخ بازنگری: 02 تیر 1402، تاریخ پذیرش: 17 تیر 1402 | ||
چکیده | ||
Drought is one of the main abiotic stresses limiting wheat growth and productivity worldwide. The main objective of this work was to determine population structure and marker-trait association (MTA) of 13 morpho-physiological traits of bread wheat for drought-tolerance breeding. To this end, twenty-five diverse wheat cultivars and promising lines were genotyped using AFLP. The phenotype evaluation steps of studding wheat genotypes were performed under normal and drought-stress conditions during 3 years. Low heritability estimates were obtained for spike length, heading date (DTH), and shoot biomass (24.87-28.8%) while, a high heritability was observed for the number of kernels per spike (KPS) (89.21-90.55%). The results exhibited high polymorphic level ranged from 84.62 to 100%, proving that AFLP method can be an effective tool in assessing genetic variability in any wheat breeding programs. Population structure analysis showed five subpopulations with at least 65% membership ancestry to their allocated sub-clusters, which was highly consistent with the results of cluster analysis. Mixed linear method association analysis identified 66 significant MTAs with p-values 10−06 to 10−04, justifying 7.8 to 38.7% of the phenotypic variation, observed under both environmental conditions. There were two pleiotropic markers for grain yield (GY) and KPS under normal and one pleiotropic marker for GY, thousand kernel weight (TKW) and KPS under stress conditions. The common MTAs were detected for DTH, plant height, peduncle length, and TKW under both environmental conditions. The identified linked markers with GY and its components in this study could be desirable candidate genes for future studies and marker assisted selection to develop drought-tolerant genotypes in wheat breeding programs. | ||
کلیدواژهها | ||
Bread wheat؛ Drought tolerance؛ Marker-trait association؛ Structure analysis | ||
عنوان مقاله [English] | ||
نقشه ارتباطی صفات مورفوفیزیولوژیکی گندم نان تحت شرایط تنش خشکی و بدون تنش | ||
نویسندگان [English] | ||
فرزاد آهک پز | ||
گروه زراعت و اصلاح نباتات، دانشگاه آزاد اسلامی، واحد میاندوآب، میاندوآب، ایران. | ||
چکیده [English] | ||
خشکی یکی از اصلیترین تنش های غیرزیستی است که رشد و بهره وری گندم را در سراسر جهان محدود میکند. هدف اصلی این پژوهش تعیین ساختار جمعیت و نقشه ارتباطی 13 صفت مورفو-فیزیولوژیکی گندم نان جهت اصلاح تحمل به خشکی بود. بدین منظور، 25 رقم و لاین امیدبخش گندم با استفاده از نشانگرهای AFLP مورد بررسی قرار گرفتند. ژنوتیپ های گندم تحت شرایط عادی و تنش خشکی به مدت 3 سال بررسی شدند. وراثتپذیری پایینی برای طول سنبله، تاریخ خوشهدهی و زیستتوده اندام هوایی (87/24-8/28٪) بدست آمد، در حالیکه، برای تعداد دانه در سنبله (21/89-55/90٪) وراثتپذیری بالایی مشاهده شد. نتایج سطح بالای چندشکلی از 62/84 تا 100 را نشان داد که ثابت میکرد روش AFLP میتواند ابزاری موثر در برنامههای اصلاحی گندم باشد. تجزیه ساختار جمعیت، پنج زیرجمعیت با حداقل 65 درصد عضویت در زیرجمعیتها را نشان داد که تطابق بالایی با نتایج حاصل از تجزیه خوشهای داشت. تجزیه ارتباط به روش خطی مختلط، 66 MTA معنیدار با مقادیر p-value از 6-10 تا 4-10 را شناسایی کرد که 8/7 تا 7/38 درصد از تغییرات فنوتیپی را در هر دو شرایط محیطی مورد مطالعه توجیه میکرد. دو نشانگر پلیوتروپیک برای عملکرد دانه و تعداد دانه در سنبله در شرایط عادی و یک نشانگر پلیوتروپیک برای عملکرد دانه، وزن هزاردانه و تعداد دانه در سنبله در شرایط تنش شناسایی شد. MTAهای مشترک برای تاریخ خوشهدهی، ارتفاع بوته، طول پدانکل و وزن هزاردانه در هر دو شرایط محیطی بدست آمد. نشانگرهای شناسایی شده مرتبط با عملکرد دانه و اجزای آن در این مطالعه میتوانند کاندیدای مطلوب برای مطالعات آتی و انتخاب به کمک نشانگر جهت توسعه ژنوتیپهای مقاوم به خشکی در برنامههای اصلاحی گندم باشند | ||
کلیدواژهها [English] | ||
ارتباط نشانگر-صفت, تجزیه ساختار, تحمل خشکی, گندم نان | ||
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